Activation of hippocampal nuclear factor-κB by retrieval is required for memory reconsolidation

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Initially, memory is labile and requires consolidation to become stable. However, several studies support that consolidated memories can undergo a new period of lability after retrieval. The mechanistic differences of this process, termed reconsolidation, with the consolidation process are under deb...

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Autores principales: Boccia, M., Freudenthal, R., Blake, M., De La Fuente, V., Acosta, G., Baratti, C., Romano, A.
Formato: Artículo publishedVersion
Lenguaje:Inglés
Publicado: 2007
Materias:
κB decoy
Hippocampus
Inhibitory avoidance
NF-κB
Reconsolidation
Sulfasalazine
double stranded DNA
immunoglobulin enhancer binding protein
salazosulfapyridine
animal experiment
article
avoidance behavior
brain function
controlled study
hippocampus
information processing
male
memory
memory consolidation
mouse
nonhuman
priority journal
task performance
Animals
Injections, Intraventricular
Male
Memory
Mice
NF-kappa B
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_02706474_v27_n49_p13436_Boccia
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling paperaa:paper_02706474_v27_n49_p13436_Boccia2023-06-12T16:47:14Z Activation of hippocampal nuclear factor-κB by retrieval is required for memory reconsolidation J. Neurosci. 2007;27(49):13436-13445 Boccia, M. Freudenthal, R. Blake, M. De La Fuente, V. Acosta, G. Baratti, C. Romano, A. κB decoy Hippocampus Inhibitory avoidance NF-κB Reconsolidation Sulfasalazine double stranded DNA immunoglobulin enhancer binding protein salazosulfapyridine animal experiment article avoidance behavior brain function controlled study hippocampus information processing male memory memory consolidation mouse nonhuman priority journal task performance Animals Hippocampus Injections, Intraventricular Male Memory Mice NF-kappa B Sulfasalazine Initially, memory is labile and requires consolidation to become stable. However, several studies support that consolidated memories can undergo a new period of lability after retrieval. The mechanistic differences of this process, termed reconsolidation, with the consolidation process are under debate, including the participation of hippocampus. Up to this point, few reports describe molecular changes and, in particular, transcription factor (TF) involvement in memory restabilization. Increasing evidence supports the participation of the TF nuclear factor-κB (NF-κB) in memory consolidation. Here, we demonstrate that the inhibition of NF-κB after memory reactivation impairs retention of a hippocampal-dependent inhibitory avoidance task in mice. We used two independent disruptive strategies to reach this conclusion. First, we administered intracerebroventricular or intrahippocampal sulfasalazine, an inhibitor of IKK (IκB kinase), the kinase that activates NF-κB. Second, we infused intracerebroventricular or intrahippocampal κB decoy, a direct inhibitor of NF-κB consisting of a double-stranded DNA oligonucleotide that contains the κB consensus sequence. When injected immediately after memory retrieval, sulfasalazine or κB decoy (Decoy) impaired long-term retention. In contrast, a one base mutated κB decoy (mDecoy) had no effect. Furthermore, we also found NF-κB activation in the hippocampus, with a peak 15 min after memory retrieval. This activation was earlier than that found during consolidation. Together, these results indicate that NF-κB is an important transcriptional regulator in memory consolidation and reconsolidation in hippocampus, although the temporal kinetics of activation differs between the two processes. Copyright © 2007 Society for Neuroscience. Fil:Freudenthal, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:De La Fuente, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Romano, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2007 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_02706474_v27_n49_p13436_Boccia
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language Inglés
orig_language_str_mv eng
topic κB decoy
Hippocampus
Inhibitory avoidance
NF-κB
Reconsolidation
Sulfasalazine
double stranded DNA
immunoglobulin enhancer binding protein
salazosulfapyridine
animal experiment
article
avoidance behavior
brain function
controlled study
hippocampus
information processing
male
memory
memory consolidation
mouse
nonhuman
priority journal
task performance
Animals
Hippocampus
Injections, Intraventricular
Male
Memory
Mice
NF-kappa B
Sulfasalazine
spellingShingle κB decoy
Hippocampus
Inhibitory avoidance
NF-κB
Reconsolidation
Sulfasalazine
double stranded DNA
immunoglobulin enhancer binding protein
salazosulfapyridine
animal experiment
article
avoidance behavior
brain function
controlled study
hippocampus
information processing
male
memory
memory consolidation
mouse
nonhuman
priority journal
task performance
Animals
Hippocampus
Injections, Intraventricular
Male
Memory
Mice
NF-kappa B
Sulfasalazine
Boccia, M.
Freudenthal, R.
Blake, M.
De La Fuente, V.
Acosta, G.
Baratti, C.
Romano, A.
Activation of hippocampal nuclear factor-κB by retrieval is required for memory reconsolidation
topic_facet κB decoy
Hippocampus
Inhibitory avoidance
NF-κB
Reconsolidation
Sulfasalazine
double stranded DNA
immunoglobulin enhancer binding protein
salazosulfapyridine
animal experiment
article
avoidance behavior
brain function
controlled study
hippocampus
information processing
male
memory
memory consolidation
mouse
nonhuman
priority journal
task performance
Animals
Hippocampus
Injections, Intraventricular
Male
Memory
Mice
NF-kappa B
Sulfasalazine
description Initially, memory is labile and requires consolidation to become stable. However, several studies support that consolidated memories can undergo a new period of lability after retrieval. The mechanistic differences of this process, termed reconsolidation, with the consolidation process are under debate, including the participation of hippocampus. Up to this point, few reports describe molecular changes and, in particular, transcription factor (TF) involvement in memory restabilization. Increasing evidence supports the participation of the TF nuclear factor-κB (NF-κB) in memory consolidation. Here, we demonstrate that the inhibition of NF-κB after memory reactivation impairs retention of a hippocampal-dependent inhibitory avoidance task in mice. We used two independent disruptive strategies to reach this conclusion. First, we administered intracerebroventricular or intrahippocampal sulfasalazine, an inhibitor of IKK (IκB kinase), the kinase that activates NF-κB. Second, we infused intracerebroventricular or intrahippocampal κB decoy, a direct inhibitor of NF-κB consisting of a double-stranded DNA oligonucleotide that contains the κB consensus sequence. When injected immediately after memory retrieval, sulfasalazine or κB decoy (Decoy) impaired long-term retention. In contrast, a one base mutated κB decoy (mDecoy) had no effect. Furthermore, we also found NF-κB activation in the hippocampus, with a peak 15 min after memory retrieval. This activation was earlier than that found during consolidation. Together, these results indicate that NF-κB is an important transcriptional regulator in memory consolidation and reconsolidation in hippocampus, although the temporal kinetics of activation differs between the two processes. Copyright © 2007 Society for Neuroscience.
format Artículo
Artículo
publishedVersion
author Boccia, M.
Freudenthal, R.
Blake, M.
De La Fuente, V.
Acosta, G.
Baratti, C.
Romano, A.
author_facet Boccia, M.
Freudenthal, R.
Blake, M.
De La Fuente, V.
Acosta, G.
Baratti, C.
Romano, A.
author_sort Boccia, M.
title Activation of hippocampal nuclear factor-κB by retrieval is required for memory reconsolidation
title_short Activation of hippocampal nuclear factor-κB by retrieval is required for memory reconsolidation
title_full Activation of hippocampal nuclear factor-κB by retrieval is required for memory reconsolidation
title_fullStr Activation of hippocampal nuclear factor-κB by retrieval is required for memory reconsolidation
title_full_unstemmed Activation of hippocampal nuclear factor-κB by retrieval is required for memory reconsolidation
title_sort activation of hippocampal nuclear factor-κb by retrieval is required for memory reconsolidation
publishDate 2007
url http://hdl.handle.net/20.500.12110/paper_02706474_v27_n49_p13436_Boccia
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AT delafuentev activationofhippocampalnuclearfactorkbbyretrievalisrequiredformemoryreconsolidation
AT acostag activationofhippocampalnuclearfactorkbbyretrievalisrequiredformemoryreconsolidation
AT barattic activationofhippocampalnuclearfactorkbbyretrievalisrequiredformemoryreconsolidation
AT romanoa activationofhippocampalnuclearfactorkbbyretrievalisrequiredformemoryreconsolidation
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