Degradation of PsbO by the Deg Protease HhoA Is Thioredoxin Dependent
Fil: Roberts, Irma N. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina.
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| Formato: | article Artículo publishedVersion |
| Lenguaje: | Inglés |
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2012
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| Acceso en línea: | http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2012Roberts |
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snrd:2012Roberts2021-10-15T16:56:07Z Roberts, Irma N. Lam, Xuan Tam Miranda, Helder Kieselbach, Thomas Funk, Christiane 2012 Fil: Roberts, Irma N. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina. The widely distributed members of the Deg/HtrA protease family play an important role in the proteolysis of misfolded and damaged proteins. Here we show that the Deg protease rHhoA is able to degrade PsbO, the extrinsic protein of the Photosystem II (PSII) oxygen-evolving complex in Synechocystis sp. PCC 6803 and in spinach. PsbO is known to be stable in its oxidized form, but after reduction by thioredoxin it became a substrate for recombinant HhoA (rHhoA). rHhoA cleaved reduced eukaryotic (specifically, spinach) PsbO at defined sites and created distinct PsbO fragments that were not further degraded. As for the corresponding prokaryotic substrate (reduced PsbO of Synechocystis sp. PCC 6803), no PsbO fragments were observed. Assembly to PSII protected PsbO from degradation. For Synechocystis sp. PCC 6803, our results show that HhoA, HhoB, and HtrA are localized in the periplasma and/or at the thylakoid membrane. In agreement with the idea that PsbO could be a physiological substrate for Deg proteases, part of the cellular fraction of the three Deg proteases of Synechocystis sp. PCC 6803 (HhoA, HhoB, and HtrA) was detected in the PSII-enriched membrane fraction. application/pdf doi:10.1371/journal.pone.0045713 issn:1932-6203 http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2012Roberts eng info:eu-repo/semantics/openAccess openAccess http://ri.agro.uba.ar/greenstone3/library/page/biblioteca#section4 Plos One Vol.7, no.9 e45713 http://www.plos.org/ BACTERIAL PROTEIN DEG PROTEASE RECOMBINANT HHOA PROTEIN HHOA PROTEIN HHOB PROTEIN HTRA PROTEIN PSBO THIOREDOXIN UNCLASSIFIED DRUG BACTERIAL CELL BACTERIAL STRAIN CELL FRACTIONATION CELLULAR DISTRIBUTION CONTROLLED STUDY CYTOPLASM ENZYME DEGRADATION ENZYME SUBSTRATE EUKARYOTE NONHUMAN NUCLEOTIDE SEQUENCE OXIDATION REDUCTION REACTION PHOTOSYSTEM II PROKARYOTE PROTEIN ASSEMBLY PROTEIN CLEAVAGE PROTEIN LOCALIZATION SPINACH SYNECHOCOCCUS THYLAKOID MEMBRANE AMINO ACID SEQUENCE BACTERIAL PROTEINS BASE SEQUENCE BLOTTING, WESTERN DNA PRIMERS ELECTROPHORESIS, POLYACRYLAMIDE GEL KINETICS MASS SPECTROMETRY MOLECULAR SEQUENCE DATA PROTEOLYSIS RECOMBINANT PROTEINS SUBCELLULAR FRACTIONS SUBSTRATE SPECIFICITY SYNECHOCYSTIS THIOREDOXINS EUKARYOTA PROKARYOTA SPINACIA OLERACEA SYNECHOCYSTIS SP. PCC 6803 Degradation of PsbO by the Deg Protease HhoA Is Thioredoxin Dependent article info:eu-repo/semantics/article info:ar-repo/semantics/artículo publishedVersion info:eu-repo/semantics/publishedVersion |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-140 |
| collection |
FAUBA Digital - Facultad de Agronomía (UBA) |
| language |
Inglés |
| orig_language_str_mv |
eng |
| topic |
BACTERIAL PROTEIN DEG PROTEASE RECOMBINANT HHOA PROTEIN HHOA PROTEIN HHOB PROTEIN HTRA PROTEIN PSBO THIOREDOXIN UNCLASSIFIED DRUG BACTERIAL CELL BACTERIAL STRAIN CELL FRACTIONATION CELLULAR DISTRIBUTION CONTROLLED STUDY CYTOPLASM ENZYME DEGRADATION ENZYME SUBSTRATE EUKARYOTE NONHUMAN NUCLEOTIDE SEQUENCE OXIDATION REDUCTION REACTION PHOTOSYSTEM II PROKARYOTE PROTEIN ASSEMBLY PROTEIN CLEAVAGE PROTEIN LOCALIZATION SPINACH SYNECHOCOCCUS THYLAKOID MEMBRANE AMINO ACID SEQUENCE BACTERIAL PROTEINS BASE SEQUENCE BLOTTING, WESTERN DNA PRIMERS ELECTROPHORESIS, POLYACRYLAMIDE GEL KINETICS MASS SPECTROMETRY MOLECULAR SEQUENCE DATA PROTEOLYSIS RECOMBINANT PROTEINS SUBCELLULAR FRACTIONS SUBSTRATE SPECIFICITY SYNECHOCYSTIS THIOREDOXINS EUKARYOTA PROKARYOTA SPINACIA OLERACEA SYNECHOCYSTIS SP. PCC 6803 |
| spellingShingle |
BACTERIAL PROTEIN DEG PROTEASE RECOMBINANT HHOA PROTEIN HHOA PROTEIN HHOB PROTEIN HTRA PROTEIN PSBO THIOREDOXIN UNCLASSIFIED DRUG BACTERIAL CELL BACTERIAL STRAIN CELL FRACTIONATION CELLULAR DISTRIBUTION CONTROLLED STUDY CYTOPLASM ENZYME DEGRADATION ENZYME SUBSTRATE EUKARYOTE NONHUMAN NUCLEOTIDE SEQUENCE OXIDATION REDUCTION REACTION PHOTOSYSTEM II PROKARYOTE PROTEIN ASSEMBLY PROTEIN CLEAVAGE PROTEIN LOCALIZATION SPINACH SYNECHOCOCCUS THYLAKOID MEMBRANE AMINO ACID SEQUENCE BACTERIAL PROTEINS BASE SEQUENCE BLOTTING, WESTERN DNA PRIMERS ELECTROPHORESIS, POLYACRYLAMIDE GEL KINETICS MASS SPECTROMETRY MOLECULAR SEQUENCE DATA PROTEOLYSIS RECOMBINANT PROTEINS SUBCELLULAR FRACTIONS SUBSTRATE SPECIFICITY SYNECHOCYSTIS THIOREDOXINS EUKARYOTA PROKARYOTA SPINACIA OLERACEA SYNECHOCYSTIS SP. PCC 6803 Roberts, Irma N. Lam, Xuan Tam Miranda, Helder Kieselbach, Thomas Funk, Christiane Degradation of PsbO by the Deg Protease HhoA Is Thioredoxin Dependent |
| topic_facet |
BACTERIAL PROTEIN DEG PROTEASE RECOMBINANT HHOA PROTEIN HHOA PROTEIN HHOB PROTEIN HTRA PROTEIN PSBO THIOREDOXIN UNCLASSIFIED DRUG BACTERIAL CELL BACTERIAL STRAIN CELL FRACTIONATION CELLULAR DISTRIBUTION CONTROLLED STUDY CYTOPLASM ENZYME DEGRADATION ENZYME SUBSTRATE EUKARYOTE NONHUMAN NUCLEOTIDE SEQUENCE OXIDATION REDUCTION REACTION PHOTOSYSTEM II PROKARYOTE PROTEIN ASSEMBLY PROTEIN CLEAVAGE PROTEIN LOCALIZATION SPINACH SYNECHOCOCCUS THYLAKOID MEMBRANE AMINO ACID SEQUENCE BACTERIAL PROTEINS BASE SEQUENCE BLOTTING, WESTERN DNA PRIMERS ELECTROPHORESIS, POLYACRYLAMIDE GEL KINETICS MASS SPECTROMETRY MOLECULAR SEQUENCE DATA PROTEOLYSIS RECOMBINANT PROTEINS SUBCELLULAR FRACTIONS SUBSTRATE SPECIFICITY SYNECHOCYSTIS THIOREDOXINS EUKARYOTA PROKARYOTA SPINACIA OLERACEA SYNECHOCYSTIS SP. PCC 6803 |
| description |
Fil: Roberts, Irma N. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina. |
| format |
article Artículo Artículo publishedVersion publishedVersion |
| author |
Roberts, Irma N. Lam, Xuan Tam Miranda, Helder Kieselbach, Thomas Funk, Christiane |
| author_facet |
Roberts, Irma N. Lam, Xuan Tam Miranda, Helder Kieselbach, Thomas Funk, Christiane |
| author_sort |
Roberts, Irma N. |
| title |
Degradation of PsbO by the Deg Protease HhoA Is Thioredoxin Dependent |
| title_short |
Degradation of PsbO by the Deg Protease HhoA Is Thioredoxin Dependent |
| title_full |
Degradation of PsbO by the Deg Protease HhoA Is Thioredoxin Dependent |
| title_fullStr |
Degradation of PsbO by the Deg Protease HhoA Is Thioredoxin Dependent |
| title_full_unstemmed |
Degradation of PsbO by the Deg Protease HhoA Is Thioredoxin Dependent |
| title_sort |
degradation of psbo by the deg protease hhoa is thioredoxin dependent |
| publishDate |
2012 |
| url |
http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2012Roberts |
| work_keys_str_mv |
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| _version_ |
1851371425357627392 |