Structural changes in the spermatophore of the freshwater 'red claw' crayfish Cherax quadricarinatus (Von Martens, 1898) (Decapoda, Parastacidae)

The structure of the spermatophore was studied in Cherax quadricarinatus. Pieces of the distal vas deferens and transferred spermatophore from the females were fixed, cut and stained. Within the distal vas deferens, the primary layer and the secondary layer of the spermatophore were distinguishable....

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Autores principales: López Greco, L.S., Lo Nostro, F.L.
Formato: JOUR
Materias:
Cherax quadricarinatus
Morphology
Red claw
Spermatophore
crayfish
cytoplasm
histology
morphology
spermatophore
Astacoidea
Parastacidae
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00017272_v89_n2_p149_LopezGreco
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00017272_v89_n2_p149_LopezGreco
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spelling todo:paper_00017272_v89_n2_p149_LopezGreco2023-10-03T13:51:50Z Structural changes in the spermatophore of the freshwater 'red claw' crayfish Cherax quadricarinatus (Von Martens, 1898) (Decapoda, Parastacidae) López Greco, L.S. Lo Nostro, F.L. Cherax quadricarinatus Morphology Red claw Spermatophore crayfish cytoplasm histology morphology spermatophore Astacoidea Cherax quadricarinatus Parastacidae The structure of the spermatophore was studied in Cherax quadricarinatus. Pieces of the distal vas deferens and transferred spermatophore from the females were fixed, cut and stained. Within the distal vas deferens, the primary layer and the secondary layer of the spermatophore were distinguishable. In the latter, two components were detected: cytoplasmic droplets and a homogeneous matrix. During the first 10 minutes post-extrusion the cytoplasmic droplets drastically changed from looking like 'empty droplets'; at this time the spermatophore changed from a liquid stage to a sticky one. One hour after extrusion the spermatophore began to harden and within the first 24-48 h post-mating it was a solid and intense white structure tightly attached to the female; after 72 h it acquired a softer aspect, completely dehiscing between 96 and 120 h post-mating. Histologically, the primary layer maintained its integrity surrounding the spermatozoa while the secondary layer lost the cytoplasmic droplets. The spermatophore began to hydrate between 24 and 48 h and by 72-96 h many sections of the sperm cord began to coalesce. From 48 h post-mating some fissures appeared within the matrix that enlarged between 72 and 120 h. We propose that both manipulation by the female and hydration are the mechanisms involved in the release of the spermatozoa from the spermatophore. © 2007 The Authors. Fil:López Greco, L.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lo Nostro, F.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00017272_v89_n2_p149_LopezGreco
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Cherax quadricarinatus
Morphology
Red claw
Spermatophore
crayfish
cytoplasm
histology
morphology
spermatophore
Astacoidea
Cherax quadricarinatus
Parastacidae
spellingShingle Cherax quadricarinatus
Morphology
Red claw
Spermatophore
crayfish
cytoplasm
histology
morphology
spermatophore
Astacoidea
Cherax quadricarinatus
Parastacidae
López Greco, L.S.
Lo Nostro, F.L.
Structural changes in the spermatophore of the freshwater 'red claw' crayfish Cherax quadricarinatus (Von Martens, 1898) (Decapoda, Parastacidae)
topic_facet Cherax quadricarinatus
Morphology
Red claw
Spermatophore
crayfish
cytoplasm
histology
morphology
spermatophore
Astacoidea
Cherax quadricarinatus
Parastacidae
description The structure of the spermatophore was studied in Cherax quadricarinatus. Pieces of the distal vas deferens and transferred spermatophore from the females were fixed, cut and stained. Within the distal vas deferens, the primary layer and the secondary layer of the spermatophore were distinguishable. In the latter, two components were detected: cytoplasmic droplets and a homogeneous matrix. During the first 10 minutes post-extrusion the cytoplasmic droplets drastically changed from looking like 'empty droplets'; at this time the spermatophore changed from a liquid stage to a sticky one. One hour after extrusion the spermatophore began to harden and within the first 24-48 h post-mating it was a solid and intense white structure tightly attached to the female; after 72 h it acquired a softer aspect, completely dehiscing between 96 and 120 h post-mating. Histologically, the primary layer maintained its integrity surrounding the spermatozoa while the secondary layer lost the cytoplasmic droplets. The spermatophore began to hydrate between 24 and 48 h and by 72-96 h many sections of the sperm cord began to coalesce. From 48 h post-mating some fissures appeared within the matrix that enlarged between 72 and 120 h. We propose that both manipulation by the female and hydration are the mechanisms involved in the release of the spermatozoa from the spermatophore. © 2007 The Authors.
format JOUR
author López Greco, L.S.
Lo Nostro, F.L.
author_facet López Greco, L.S.
Lo Nostro, F.L.
author_sort López Greco, L.S.
title Structural changes in the spermatophore of the freshwater 'red claw' crayfish Cherax quadricarinatus (Von Martens, 1898) (Decapoda, Parastacidae)
title_short Structural changes in the spermatophore of the freshwater 'red claw' crayfish Cherax quadricarinatus (Von Martens, 1898) (Decapoda, Parastacidae)
title_full Structural changes in the spermatophore of the freshwater 'red claw' crayfish Cherax quadricarinatus (Von Martens, 1898) (Decapoda, Parastacidae)
title_fullStr Structural changes in the spermatophore of the freshwater 'red claw' crayfish Cherax quadricarinatus (Von Martens, 1898) (Decapoda, Parastacidae)
title_full_unstemmed Structural changes in the spermatophore of the freshwater 'red claw' crayfish Cherax quadricarinatus (Von Martens, 1898) (Decapoda, Parastacidae)
title_sort structural changes in the spermatophore of the freshwater 'red claw' crayfish cherax quadricarinatus (von martens, 1898) (decapoda, parastacidae)
url http://hdl.handle.net/20.500.12110/paper_00017272_v89_n2_p149_LopezGreco
work_keys_str_mv AT lopezgrecols structuralchangesinthespermatophoreofthefreshwaterredclawcrayfishcheraxquadricarinatusvonmartens1898decapodaparastacidae
AT lonostrofl structuralchangesinthespermatophoreofthefreshwaterredclawcrayfishcheraxquadricarinatusvonmartens1898decapodaparastacidae
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