Chemical two-photon fluorescence

We describe a method based on a caged fluorescent molecule that can act as a chemical two-photon probe. It is composed of an organic fluorophore and a ruthenium-bipyridine complex that acts as a photoremovable quencher. For the fluorophore to be emissive, two independent photons must act on the mole...

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Autores principales:	Carrone, G., Etchenique, R.
Formato:	JOUR
Materias:	Fluorescence Fluorophores High power lasers Molecules Probes Pulsed lasers Ruthenium compounds Ultrashort pulses Excitation intensity Fluorescent molecules Microscopy technique Organic fluorophores Ruthenium-bipyridine complexes Two photon fluorescence Two-photon excitations Ultra-short pulsed Photons
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00032700_v87_n8_p4363_Carrone
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00032700_v87_n8_p4363_Carrone
record_format	dspace
spelling	todo:paper_00032700_v87_n8_p4363_Carrone2023-10-03T13:56:05Z Chemical two-photon fluorescence Carrone, G. Etchenique, R. Fluorescence Fluorophores High power lasers Molecules Probes Pulsed lasers Ruthenium compounds Ultrashort pulses Excitation intensity Fluorescent molecules Microscopy technique Organic fluorophores Ruthenium-bipyridine complexes Two photon fluorescence Two-photon excitations Ultra-short pulsed Photons We describe a method based on a caged fluorescent molecule that can act as a chemical two-photon probe. It is composed of an organic fluorophore and a ruthenium-bipyridine complex that acts as a photoremovable quencher. For the fluorophore to be emissive, two independent photons must act on the molecule: the first photon frees the fluorescent ligand from the Ru complex and the second photon excites the fluorescence. In this two-photon regime, the emission is not proportional to the excitation intensity but rather to its second power, as in traditional two-photon systems based on ultrashort pulsed high-power lasers. This quadratic relationship implies a much higher spatial precision on the z-axis when the probe is used in a microscopy technique. The chemical nature of the two-photon excitation mechanism allows the use of inexpensive low-power lasers. © 2015 American Chemical Society. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00032700_v87_n8_p4363_Carrone
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Fluorescence Fluorophores High power lasers Molecules Probes Pulsed lasers Ruthenium compounds Ultrashort pulses Excitation intensity Fluorescent molecules Microscopy technique Organic fluorophores Ruthenium-bipyridine complexes Two photon fluorescence Two-photon excitations Ultra-short pulsed Photons
spellingShingle	Fluorescence Fluorophores High power lasers Molecules Probes Pulsed lasers Ruthenium compounds Ultrashort pulses Excitation intensity Fluorescent molecules Microscopy technique Organic fluorophores Ruthenium-bipyridine complexes Two photon fluorescence Two-photon excitations Ultra-short pulsed Photons Carrone, G. Etchenique, R. Chemical two-photon fluorescence
topic_facet	Fluorescence Fluorophores High power lasers Molecules Probes Pulsed lasers Ruthenium compounds Ultrashort pulses Excitation intensity Fluorescent molecules Microscopy technique Organic fluorophores Ruthenium-bipyridine complexes Two photon fluorescence Two-photon excitations Ultra-short pulsed Photons
description	We describe a method based on a caged fluorescent molecule that can act as a chemical two-photon probe. It is composed of an organic fluorophore and a ruthenium-bipyridine complex that acts as a photoremovable quencher. For the fluorophore to be emissive, two independent photons must act on the molecule: the first photon frees the fluorescent ligand from the Ru complex and the second photon excites the fluorescence. In this two-photon regime, the emission is not proportional to the excitation intensity but rather to its second power, as in traditional two-photon systems based on ultrashort pulsed high-power lasers. This quadratic relationship implies a much higher spatial precision on the z-axis when the probe is used in a microscopy technique. The chemical nature of the two-photon excitation mechanism allows the use of inexpensive low-power lasers. © 2015 American Chemical Society.
format	JOUR
author	Carrone, G. Etchenique, R.
author_facet	Carrone, G. Etchenique, R.
author_sort	Carrone, G.
title	Chemical two-photon fluorescence
title_short	Chemical two-photon fluorescence
title_full	Chemical two-photon fluorescence
title_fullStr	Chemical two-photon fluorescence
title_full_unstemmed	Chemical two-photon fluorescence
title_sort	chemical two-photon fluorescence
url	http://hdl.handle.net/20.500.12110/paper_00032700_v87_n8_p4363_Carrone
work_keys_str_mv	AT carroneg chemicaltwophotonfluorescence AT etcheniquer chemicaltwophotonfluorescence
_version_	1807317391308226560

Chemical two-photon fluorescence

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