Porphyrin biosynthesis in soybean callus. V. The porphobilinogen deaminase-uroporphyrinogen cosynthetase system. Kinetic studies

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Kinetic studies were carried out using purified porphobilinogenase and deaminase preparations in the presence and absence of ammonium ions. It has been found in plots of v versus [S] that a deviation from the Michaelis-Menten hyperbola occurs with both enzymes; double-reciprocal plots were concave d...

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Autores principales: Llambías, E.B.C., Del C. Batlle, A.M.
Formato: JOUR
Materias:
ammonium derivative
article
binding site
Ammonium Compounds
Binding Sites
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00052744_v220_n3_p552_Llambias
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00052744_v220_n3_p552_Llambias
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spelling todo:paper_00052744_v220_n3_p552_Llambias2023-10-03T14:03:25Z Porphyrin biosynthesis in soybean callus. V. The porphobilinogen deaminase-uroporphyrinogen cosynthetase system. Kinetic studies Llambías, E.B.C. Del C. Batlle, A.M. ammonium derivative article binding site Ammonium Compounds Binding Sites Kinetic studies were carried out using purified porphobilinogenase and deaminase preparations in the presence and absence of ammonium ions. It has been found in plots of v versus [S] that a deviation from the Michaelis-Menten hyperbola occurs with both enzymes; double-reciprocal plots were concave downward; Rs values were greater than 81; and in some cases the Hill coefficient was less than 1, indicating negative homotropic kinetics. Evidence also suggested that porphobilinogenase contains at least two substrate-binding sites per molecule of enzyme. It has also been found that ammonium ions act competitively on the first reaction of the porphobilinogenase. © 1970. Fil:Llambías, E.B.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Del C. Batlle, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00052744_v220_n3_p552_Llambias
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic ammonium derivative
article
binding site
Ammonium Compounds
Binding Sites
spellingShingle ammonium derivative
article
binding site
Ammonium Compounds
Binding Sites
Llambías, E.B.C.
Del C. Batlle, A.M.
Porphyrin biosynthesis in soybean callus. V. The porphobilinogen deaminase-uroporphyrinogen cosynthetase system. Kinetic studies
topic_facet ammonium derivative
article
binding site
Ammonium Compounds
Binding Sites
description Kinetic studies were carried out using purified porphobilinogenase and deaminase preparations in the presence and absence of ammonium ions. It has been found in plots of v versus [S] that a deviation from the Michaelis-Menten hyperbola occurs with both enzymes; double-reciprocal plots were concave downward; Rs values were greater than 81; and in some cases the Hill coefficient was less than 1, indicating negative homotropic kinetics. Evidence also suggested that porphobilinogenase contains at least two substrate-binding sites per molecule of enzyme. It has also been found that ammonium ions act competitively on the first reaction of the porphobilinogenase. © 1970.
format JOUR
author Llambías, E.B.C.
Del C. Batlle, A.M.
author_facet Llambías, E.B.C.
Del C. Batlle, A.M.
author_sort Llambías, E.B.C.
title Porphyrin biosynthesis in soybean callus. V. The porphobilinogen deaminase-uroporphyrinogen cosynthetase system. Kinetic studies
title_short Porphyrin biosynthesis in soybean callus. V. The porphobilinogen deaminase-uroporphyrinogen cosynthetase system. Kinetic studies
title_full Porphyrin biosynthesis in soybean callus. V. The porphobilinogen deaminase-uroporphyrinogen cosynthetase system. Kinetic studies
title_fullStr Porphyrin biosynthesis in soybean callus. V. The porphobilinogen deaminase-uroporphyrinogen cosynthetase system. Kinetic studies
title_full_unstemmed Porphyrin biosynthesis in soybean callus. V. The porphobilinogen deaminase-uroporphyrinogen cosynthetase system. Kinetic studies
title_sort porphyrin biosynthesis in soybean callus. v. the porphobilinogen deaminase-uroporphyrinogen cosynthetase system. kinetic studies
url http://hdl.handle.net/20.500.12110/paper_00052744_v220_n3_p552_Llambias
work_keys_str_mv AT llambiasebc porphyrinbiosynthesisinsoybeancallusvtheporphobilinogendeaminaseuroporphyrinogencosynthetasesystemkineticstudies
AT delcbatlleam porphyrinbiosynthesisinsoybeancallusvtheporphobilinogendeaminaseuroporphyrinogencosynthetasesystemkineticstudies
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