Sequence analysis, expression, and paratope characterization of a single-chain Fv fragment for the eukaryote ribosomal P proteins

The variable genes of monoclonal antibody (mAb) B10, specific for the C-terminal region of the eukaryotic ribosomal P protein, have been cloned as a single-chain Fv fragment (scFv) and expressed in Escherichia coli. The primary sequence of the variable regions of the B10 antibody, together with a de...

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Detalles Bibliográficos
Autores principales:	López Bergami, P., Mateos, P., Hoebeke, J., Levin, M.J., Baldi, A.
Formato:	JOUR
Materias:	Autoantibody Paratope-epitope modelling Ribosomal P protein Single-chain Fv fragment Trypanosoma cruzi autoantibody epitope monoclonal antibody ribosome protein antibody combining site article binding affinity carboxy terminal sequence Escherichia coli eukaryote molecular biology molecular cloning molecular interaction nonhuman nucleotide sequence priority journal protein analysis protein expression sequence analysis Eukaryota Trypanosoma
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_0006291X_v301_n4_p819_LopezBergami
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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