Protein interactions within the TcZFP zinc finger family members of Trypanosoma cruzi: Implications for their functions
The small zinc finger proteins tbZFP1 and tbZFP2 have been implicated in the control of Trypanosoma brucei differentiation to the procyclic form. Here, we report that the complete ZFP family in Trypanosoma cruzi is composed by four members, ZFP1A and B, and ZFP2A and B. ZFP1B is a paralog specific g...
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todo:paper_0006291X_v333_n3_p1017_Caro2023-10-03T14:03:50Z Protein interactions within the TcZFP zinc finger family members of Trypanosoma cruzi: Implications for their functions Caro, F. Bercovich, N. Atorrasagasti, C. Levin, M.J. Vázquez, M.P. CCCH zinc finger proteins Chagas disease Protein interactions WW domains glycine unclassified drug zinc finger protein zinc finger protein 1a zinc finger protein 1b zinc finger protein 2a zinc finger protein 2b article dimerization genetic line genetic variability molecular model nucleotide sequence paralogy priority journal protein analysis protein domain protein family protein function protein protein interaction Trypanosoma cruzi Amino Acid Sequence Animals Base Sequence Dimerization DNA, Protozoan Molecular Sequence Data Protein Binding Protozoan Proteins Reverse Transcriptase Polymerase Chain Reaction Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid Trypanosoma cruzi Zinc Fingers Trypanosoma brucei Trypanosoma cruzi Trypanosomatidae The small zinc finger proteins tbZFP1 and tbZFP2 have been implicated in the control of Trypanosoma brucei differentiation to the procyclic form. Here, we report that the complete ZFP family in Trypanosoma cruzi is composed by four members, ZFP1A and B, and ZFP2A and B. ZFP1B is a paralog specific gene restricted to T. cruzi, while the ZFP2A and B paralogs diverged prior to the trypanosomatid lineage separation. Moreover, we demonstrate that TcZFP1 and TcZFP2 members interact with each other and that this interaction is mediated by a WW domain in TcZFP2. Also, TcZFP2B strongly homodimerizes by a glycine rich region absent in TcZFP2A. We propose a model to discuss the relevance of these protein-protein interactions in terms of the functions of these proteins. © 2005 Elsevier Inc. All rights reserved. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0006291X_v333_n3_p1017_Caro |
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Universidad de Buenos Aires |
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I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
CCCH zinc finger proteins Chagas disease Protein interactions WW domains glycine unclassified drug zinc finger protein zinc finger protein 1a zinc finger protein 1b zinc finger protein 2a zinc finger protein 2b article dimerization genetic line genetic variability molecular model nucleotide sequence paralogy priority journal protein analysis protein domain protein family protein function protein protein interaction Trypanosoma cruzi Amino Acid Sequence Animals Base Sequence Dimerization DNA, Protozoan Molecular Sequence Data Protein Binding Protozoan Proteins Reverse Transcriptase Polymerase Chain Reaction Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid Trypanosoma cruzi Zinc Fingers Trypanosoma brucei Trypanosoma cruzi Trypanosomatidae |
| spellingShingle |
CCCH zinc finger proteins Chagas disease Protein interactions WW domains glycine unclassified drug zinc finger protein zinc finger protein 1a zinc finger protein 1b zinc finger protein 2a zinc finger protein 2b article dimerization genetic line genetic variability molecular model nucleotide sequence paralogy priority journal protein analysis protein domain protein family protein function protein protein interaction Trypanosoma cruzi Amino Acid Sequence Animals Base Sequence Dimerization DNA, Protozoan Molecular Sequence Data Protein Binding Protozoan Proteins Reverse Transcriptase Polymerase Chain Reaction Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid Trypanosoma cruzi Zinc Fingers Trypanosoma brucei Trypanosoma cruzi Trypanosomatidae Caro, F. Bercovich, N. Atorrasagasti, C. Levin, M.J. Vázquez, M.P. Protein interactions within the TcZFP zinc finger family members of Trypanosoma cruzi: Implications for their functions |
| topic_facet |
CCCH zinc finger proteins Chagas disease Protein interactions WW domains glycine unclassified drug zinc finger protein zinc finger protein 1a zinc finger protein 1b zinc finger protein 2a zinc finger protein 2b article dimerization genetic line genetic variability molecular model nucleotide sequence paralogy priority journal protein analysis protein domain protein family protein function protein protein interaction Trypanosoma cruzi Amino Acid Sequence Animals Base Sequence Dimerization DNA, Protozoan Molecular Sequence Data Protein Binding Protozoan Proteins Reverse Transcriptase Polymerase Chain Reaction Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid Trypanosoma cruzi Zinc Fingers Trypanosoma brucei Trypanosoma cruzi Trypanosomatidae |
| description |
The small zinc finger proteins tbZFP1 and tbZFP2 have been implicated in the control of Trypanosoma brucei differentiation to the procyclic form. Here, we report that the complete ZFP family in Trypanosoma cruzi is composed by four members, ZFP1A and B, and ZFP2A and B. ZFP1B is a paralog specific gene restricted to T. cruzi, while the ZFP2A and B paralogs diverged prior to the trypanosomatid lineage separation. Moreover, we demonstrate that TcZFP1 and TcZFP2 members interact with each other and that this interaction is mediated by a WW domain in TcZFP2. Also, TcZFP2B strongly homodimerizes by a glycine rich region absent in TcZFP2A. We propose a model to discuss the relevance of these protein-protein interactions in terms of the functions of these proteins. © 2005 Elsevier Inc. All rights reserved. |
| format |
JOUR |
| author |
Caro, F. Bercovich, N. Atorrasagasti, C. Levin, M.J. Vázquez, M.P. |
| author_facet |
Caro, F. Bercovich, N. Atorrasagasti, C. Levin, M.J. Vázquez, M.P. |
| author_sort |
Caro, F. |
| title |
Protein interactions within the TcZFP zinc finger family members of Trypanosoma cruzi: Implications for their functions |
| title_short |
Protein interactions within the TcZFP zinc finger family members of Trypanosoma cruzi: Implications for their functions |
| title_full |
Protein interactions within the TcZFP zinc finger family members of Trypanosoma cruzi: Implications for their functions |
| title_fullStr |
Protein interactions within the TcZFP zinc finger family members of Trypanosoma cruzi: Implications for their functions |
| title_full_unstemmed |
Protein interactions within the TcZFP zinc finger family members of Trypanosoma cruzi: Implications for their functions |
| title_sort |
protein interactions within the tczfp zinc finger family members of trypanosoma cruzi: implications for their functions |
| url |
http://hdl.handle.net/20.500.12110/paper_0006291X_v333_n3_p1017_Caro |
| work_keys_str_mv |
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