Molecular and functional characterization of a spermidine transporter (TcPAT12) from Trypanosoma cruzi
Trypanosoma cruzi, the etiological agent of Chagas' disease, is the only eukaryotic cell which lacks the ability to synthesize polyamines de novo. In this work, we describe for the first time the molecular and biochemical properties of a high-affinity spermidine transporter from T. cruzi. The t...
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todo:paper_0006291X_v344_n3_p936_Carrillo2023-10-03T14:03:51Z Molecular and functional characterization of a spermidine transporter (TcPAT12) from Trypanosoma cruzi Carrillo, C. Canepa, G.E. Algranati, I.D. Pereira, C.A. Arginine Chagas' disease Permease Polyamines Putrescine Spermidine Trypanosoma cruzi amino acid arginine polyamine putrescine spermidine spermidine transporter unclassified drug article epimastigote gene expression nucleotide sequence polyamine synthesis priority journal protein determination protein expression protein transport Trypanosoma cruzi Xenopus laevis Amino Acid Sequence Animals Molecular Sequence Data Protein Binding Protozoan Proteins Spermidine Trypanosoma cruzi Eukaryota Trypanosoma cruzi Xenopus laevis Trypanosoma cruzi, the etiological agent of Chagas' disease, is the only eukaryotic cell which lacks the ability to synthesize polyamines de novo. In this work, we describe for the first time the molecular and biochemical properties of a high-affinity spermidine transporter from T. cruzi. The transporter gene TcPAT12 was functionally expressed in Xenopus laevis oocytes, showing high levels of spermidine uptake. Similar apparent affinity constants for spermidine uptake were obtained when comparing T. cruzi epimastigotes and heterologous expressed TcPAT12 in X. laevis. In addition, TcPAT12 also transports putrescine and the amino acid l-arginine at lower rates than spermidine. © 2006 Elsevier Inc. All rights reserved. Fil:Carrillo, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Canepa, G.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Algranati, I.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pereira, C.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0006291X_v344_n3_p936_Carrillo |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Arginine Chagas' disease Permease Polyamines Putrescine Spermidine Trypanosoma cruzi amino acid arginine polyamine putrescine spermidine spermidine transporter unclassified drug article epimastigote gene expression nucleotide sequence polyamine synthesis priority journal protein determination protein expression protein transport Trypanosoma cruzi Xenopus laevis Amino Acid Sequence Animals Molecular Sequence Data Protein Binding Protozoan Proteins Spermidine Trypanosoma cruzi Eukaryota Trypanosoma cruzi Xenopus laevis |
| spellingShingle |
Arginine Chagas' disease Permease Polyamines Putrescine Spermidine Trypanosoma cruzi amino acid arginine polyamine putrescine spermidine spermidine transporter unclassified drug article epimastigote gene expression nucleotide sequence polyamine synthesis priority journal protein determination protein expression protein transport Trypanosoma cruzi Xenopus laevis Amino Acid Sequence Animals Molecular Sequence Data Protein Binding Protozoan Proteins Spermidine Trypanosoma cruzi Eukaryota Trypanosoma cruzi Xenopus laevis Carrillo, C. Canepa, G.E. Algranati, I.D. Pereira, C.A. Molecular and functional characterization of a spermidine transporter (TcPAT12) from Trypanosoma cruzi |
| topic_facet |
Arginine Chagas' disease Permease Polyamines Putrescine Spermidine Trypanosoma cruzi amino acid arginine polyamine putrescine spermidine spermidine transporter unclassified drug article epimastigote gene expression nucleotide sequence polyamine synthesis priority journal protein determination protein expression protein transport Trypanosoma cruzi Xenopus laevis Amino Acid Sequence Animals Molecular Sequence Data Protein Binding Protozoan Proteins Spermidine Trypanosoma cruzi Eukaryota Trypanosoma cruzi Xenopus laevis |
| description |
Trypanosoma cruzi, the etiological agent of Chagas' disease, is the only eukaryotic cell which lacks the ability to synthesize polyamines de novo. In this work, we describe for the first time the molecular and biochemical properties of a high-affinity spermidine transporter from T. cruzi. The transporter gene TcPAT12 was functionally expressed in Xenopus laevis oocytes, showing high levels of spermidine uptake. Similar apparent affinity constants for spermidine uptake were obtained when comparing T. cruzi epimastigotes and heterologous expressed TcPAT12 in X. laevis. In addition, TcPAT12 also transports putrescine and the amino acid l-arginine at lower rates than spermidine. © 2006 Elsevier Inc. All rights reserved. |
| format |
JOUR |
| author |
Carrillo, C. Canepa, G.E. Algranati, I.D. Pereira, C.A. |
| author_facet |
Carrillo, C. Canepa, G.E. Algranati, I.D. Pereira, C.A. |
| author_sort |
Carrillo, C. |
| title |
Molecular and functional characterization of a spermidine transporter (TcPAT12) from Trypanosoma cruzi |
| title_short |
Molecular and functional characterization of a spermidine transporter (TcPAT12) from Trypanosoma cruzi |
| title_full |
Molecular and functional characterization of a spermidine transporter (TcPAT12) from Trypanosoma cruzi |
| title_fullStr |
Molecular and functional characterization of a spermidine transporter (TcPAT12) from Trypanosoma cruzi |
| title_full_unstemmed |
Molecular and functional characterization of a spermidine transporter (TcPAT12) from Trypanosoma cruzi |
| title_sort |
molecular and functional characterization of a spermidine transporter (tcpat12) from trypanosoma cruzi |
| url |
http://hdl.handle.net/20.500.12110/paper_0006291X_v344_n3_p936_Carrillo |
| work_keys_str_mv |
AT carrilloc molecularandfunctionalcharacterizationofaspermidinetransportertcpat12fromtrypanosomacruzi AT canepage molecularandfunctionalcharacterizationofaspermidinetransportertcpat12fromtrypanosomacruzi AT algranatiid molecularandfunctionalcharacterizationofaspermidinetransportertcpat12fromtrypanosomacruzi AT pereiraca molecularandfunctionalcharacterizationofaspermidinetransportertcpat12fromtrypanosomacruzi |
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1807317114927710208 |