Autocrine/paracrine involvement of insulin-like growth factor-I and its receptor in chronic lymphocytic leukaemia
Chronic lymphocytic leukaemia (CLL) is characterized by the accumulation of long-lived B lymphocytes blocked in G0/1 by impaired apoptosis. As insulin-like growth factor-I (IGF-I) is known for its antiapoptotic effects in different cell types, we investigated whether IGF-I and its receptor (IGF-IR)...
Guardado en:
| Autores principales: | , , , , , |
|---|---|
| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00071048_v130_n1_p58_Schillaci |
| Aporte de: |
| id |
todo:paper_00071048_v130_n1_p58_Schillaci |
|---|---|
| record_format |
dspace |
| spelling |
todo:paper_00071048_v130_n1_p58_Schillaci2023-10-03T14:05:29Z Autocrine/paracrine involvement of insulin-like growth factor-I and its receptor in chronic lymphocytic leukaemia Schillaci, R. Galeano, A. Becu-Villalobos, D. Spinelli, O. Sapia, S. Bezares, R.F. B cells Chronic lymphocytic leukaemia (CLL) CLL apoptosis CLL growth factors growth hormone messenger RNA protein bcl 2 somatomedin C somatomedin C receptor adult aged apoptosis article autocrine effect B lymphocyte cell cycle cell survival chronic lymphatic leukemia clinical article controlled study female flow cytometry human human cell immunophenotyping male paracrine signaling priority journal protein expression reverse transcription polymerase chain reaction statistical analysis Aged Aged, 80 and over Analysis of Variance Apoptosis Autocrine Communication B-Lymphocytes Case-Control Studies Culture Media, Conditioned Female Flow Cytometry Growth Hormone Humans Insulin-Like Growth Factor I Leukemia, Lymphocytic, Chronic Male Middle Aged Paracrine Communication Receptor, IGF Type 1 Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger Tumor Cells, Cultured Chronic lymphocytic leukaemia (CLL) is characterized by the accumulation of long-lived B lymphocytes blocked in G0/1 by impaired apoptosis. As insulin-like growth factor-I (IGF-I) is known for its antiapoptotic effects in different cell types, we investigated whether IGF-I and its receptor (IGF-IR) participate in autocrine/paracrine loops affecting the survival of CLL cells. IGF-IR protein and mRNA was present in CLL cells in 44% and 59% of patients respectively. IGF-IR expression in CLL patients was positively correlated with the expression of the antiapoptotic protein Bcl-2 and was involved in CLL cell survival in vitro. Serum IGF-I was elevated in CLL patients, but growth hormone (GH) was normal. CLL cells expressed IGF-I mRNA and secreted the growth factor in vitro. Therefore, local production of IGF-I can account for the increased levels of serum IGF-I, independently of GH, and may be related to autocrine/paracrine control of lymphocyte survival acting at IGF-IR. This is the first demonstration of IGF-IR expression in a subgroup of CLL patients and of its antiapoptotic effects in vitro, highlighting the importance of this growth factor receptor as a possible therapeutic target in CLL. © 2005 Blackwell Publishing Ltd. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00071048_v130_n1_p58_Schillaci |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
B cells Chronic lymphocytic leukaemia (CLL) CLL apoptosis CLL growth factors growth hormone messenger RNA protein bcl 2 somatomedin C somatomedin C receptor adult aged apoptosis article autocrine effect B lymphocyte cell cycle cell survival chronic lymphatic leukemia clinical article controlled study female flow cytometry human human cell immunophenotyping male paracrine signaling priority journal protein expression reverse transcription polymerase chain reaction statistical analysis Aged Aged, 80 and over Analysis of Variance Apoptosis Autocrine Communication B-Lymphocytes Case-Control Studies Culture Media, Conditioned Female Flow Cytometry Growth Hormone Humans Insulin-Like Growth Factor I Leukemia, Lymphocytic, Chronic Male Middle Aged Paracrine Communication Receptor, IGF Type 1 Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger Tumor Cells, Cultured |
| spellingShingle |
B cells Chronic lymphocytic leukaemia (CLL) CLL apoptosis CLL growth factors growth hormone messenger RNA protein bcl 2 somatomedin C somatomedin C receptor adult aged apoptosis article autocrine effect B lymphocyte cell cycle cell survival chronic lymphatic leukemia clinical article controlled study female flow cytometry human human cell immunophenotyping male paracrine signaling priority journal protein expression reverse transcription polymerase chain reaction statistical analysis Aged Aged, 80 and over Analysis of Variance Apoptosis Autocrine Communication B-Lymphocytes Case-Control Studies Culture Media, Conditioned Female Flow Cytometry Growth Hormone Humans Insulin-Like Growth Factor I Leukemia, Lymphocytic, Chronic Male Middle Aged Paracrine Communication Receptor, IGF Type 1 Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger Tumor Cells, Cultured Schillaci, R. Galeano, A. Becu-Villalobos, D. Spinelli, O. Sapia, S. Bezares, R.F. Autocrine/paracrine involvement of insulin-like growth factor-I and its receptor in chronic lymphocytic leukaemia |
| topic_facet |
B cells Chronic lymphocytic leukaemia (CLL) CLL apoptosis CLL growth factors growth hormone messenger RNA protein bcl 2 somatomedin C somatomedin C receptor adult aged apoptosis article autocrine effect B lymphocyte cell cycle cell survival chronic lymphatic leukemia clinical article controlled study female flow cytometry human human cell immunophenotyping male paracrine signaling priority journal protein expression reverse transcription polymerase chain reaction statistical analysis Aged Aged, 80 and over Analysis of Variance Apoptosis Autocrine Communication B-Lymphocytes Case-Control Studies Culture Media, Conditioned Female Flow Cytometry Growth Hormone Humans Insulin-Like Growth Factor I Leukemia, Lymphocytic, Chronic Male Middle Aged Paracrine Communication Receptor, IGF Type 1 Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger Tumor Cells, Cultured |
| description |
Chronic lymphocytic leukaemia (CLL) is characterized by the accumulation of long-lived B lymphocytes blocked in G0/1 by impaired apoptosis. As insulin-like growth factor-I (IGF-I) is known for its antiapoptotic effects in different cell types, we investigated whether IGF-I and its receptor (IGF-IR) participate in autocrine/paracrine loops affecting the survival of CLL cells. IGF-IR protein and mRNA was present in CLL cells in 44% and 59% of patients respectively. IGF-IR expression in CLL patients was positively correlated with the expression of the antiapoptotic protein Bcl-2 and was involved in CLL cell survival in vitro. Serum IGF-I was elevated in CLL patients, but growth hormone (GH) was normal. CLL cells expressed IGF-I mRNA and secreted the growth factor in vitro. Therefore, local production of IGF-I can account for the increased levels of serum IGF-I, independently of GH, and may be related to autocrine/paracrine control of lymphocyte survival acting at IGF-IR. This is the first demonstration of IGF-IR expression in a subgroup of CLL patients and of its antiapoptotic effects in vitro, highlighting the importance of this growth factor receptor as a possible therapeutic target in CLL. © 2005 Blackwell Publishing Ltd. |
| format |
JOUR |
| author |
Schillaci, R. Galeano, A. Becu-Villalobos, D. Spinelli, O. Sapia, S. Bezares, R.F. |
| author_facet |
Schillaci, R. Galeano, A. Becu-Villalobos, D. Spinelli, O. Sapia, S. Bezares, R.F. |
| author_sort |
Schillaci, R. |
| title |
Autocrine/paracrine involvement of insulin-like growth factor-I and its receptor in chronic lymphocytic leukaemia |
| title_short |
Autocrine/paracrine involvement of insulin-like growth factor-I and its receptor in chronic lymphocytic leukaemia |
| title_full |
Autocrine/paracrine involvement of insulin-like growth factor-I and its receptor in chronic lymphocytic leukaemia |
| title_fullStr |
Autocrine/paracrine involvement of insulin-like growth factor-I and its receptor in chronic lymphocytic leukaemia |
| title_full_unstemmed |
Autocrine/paracrine involvement of insulin-like growth factor-I and its receptor in chronic lymphocytic leukaemia |
| title_sort |
autocrine/paracrine involvement of insulin-like growth factor-i and its receptor in chronic lymphocytic leukaemia |
| url |
http://hdl.handle.net/20.500.12110/paper_00071048_v130_n1_p58_Schillaci |
| work_keys_str_mv |
AT schillacir autocrineparacrineinvolvementofinsulinlikegrowthfactorianditsreceptorinchroniclymphocyticleukaemia AT galeanoa autocrineparacrineinvolvementofinsulinlikegrowthfactorianditsreceptorinchroniclymphocyticleukaemia AT becuvillalobosd autocrineparacrineinvolvementofinsulinlikegrowthfactorianditsreceptorinchroniclymphocyticleukaemia AT spinellio autocrineparacrineinvolvementofinsulinlikegrowthfactorianditsreceptorinchroniclymphocyticleukaemia AT sapias autocrineparacrineinvolvementofinsulinlikegrowthfactorianditsreceptorinchroniclymphocyticleukaemia AT bezaresrf autocrineparacrineinvolvementofinsulinlikegrowthfactorianditsreceptorinchroniclymphocyticleukaemia |
| _version_ |
1807321907552321536 |