Fibroblastic colony-forming units and levels of tumor necrosis factor and prostaglandin E2 in bone marrow cultures from patients with advanced lung carcinoma
BACKGROUND. Although alterations of the bone marrow (BM) fibroblast colony-forming cells are involved in the development of diverse hematologic disorders, these progenitors still have not been well characterized in patients with solid tumors. METHODS. The incidence of fibroblast colony- forming unit...
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todo:paper_0008543X_v80_n10_p1914_Chasseing2023-10-03T14:06:11Z Fibroblastic colony-forming units and levels of tumor necrosis factor and prostaglandin E2 in bone marrow cultures from patients with advanced lung carcinoma Chasseing, N.A. Bordenave, R.H. Bullorsky, E.O. Diaz, N.B. Stemmelin, G.R. Rumi, L.S. Bone marrow fibroblast Lung carcinoma Prostaglandin E2 Tumor necrosis factor-α prostaglandin e2 tumor necrosis factor advanced cancer article bone marrow culture colony forming unit f controlled study enzyme linked immunosorbent assay human human tissue lung non small cell cancer mononuclear cell priority journal radioimmunoassay Bone Marrow Cells Carcinoma, Non-Small-Cell Lung Case-Control Studies Colony-Forming Units Assay Culture Media, Conditioned Dinoprostone Female Fibroblasts Humans Lung Neoplasms Male Stem Cells Tumor Necrosis Factor-alpha BACKGROUND. Although alterations of the bone marrow (BM) fibroblast colony-forming cells are involved in the development of diverse hematologic disorders, these progenitors still have not been well characterized in patients with solid tumors. METHODS. The incidence of fibroblast colony- forming units (CFU-F) was evaluated in the cultures of unseparated and fractionated light density BM mononuclear cells (MC) from 25 consecutive untreated lung carcinoma patients (LCP) and 16 normal controls (NC). Unseparated MC also were cultured in the presence of indomethacin (10-6 M). Finally, the authors evaluated the spontaneous production of prostaglandin E2 (PGE2) and tumor necrosis factor-α (TNF-α) in culture conditioned mediums of unseparated MC by radioimmunoassay and enzyme-linked immunoadsorbent assay methodology, respectively. RESULTS. A decreased number of CFU-F was observed in unseparated and fractionated (adherent and nonadherent) light density MC cultures from LCP compared with NC. When unseparated MC of LCP were treated with indomethacin, a slightly increase in the number of CFU-F was found. Adherent MC (stromal cells) achieved confluence only in 44% of LCP primary cultures compared with 100% of NC. Overproduction of PGE2 and TNF-α was found in the conditioned mediums of LCP compared with the mean values obtained in NC (P < 0.05 and P < 0.02, respectively). CONCLUSIONS. The lack of confluence and suppression of CFU-F in BM of LCP may be related to the increase production of PGE2 and TNF-α. Future investigation will allow the determination of how these modifications influence tumor cell growth and will prove if more alterations of the hematopoietic microenvironment imply a worse prognosis. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0008543X_v80_n10_p1914_Chasseing |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Bone marrow fibroblast Lung carcinoma Prostaglandin E2 Tumor necrosis factor-α prostaglandin e2 tumor necrosis factor advanced cancer article bone marrow culture colony forming unit f controlled study enzyme linked immunosorbent assay human human tissue lung non small cell cancer mononuclear cell priority journal radioimmunoassay Bone Marrow Cells Carcinoma, Non-Small-Cell Lung Case-Control Studies Colony-Forming Units Assay Culture Media, Conditioned Dinoprostone Female Fibroblasts Humans Lung Neoplasms Male Stem Cells Tumor Necrosis Factor-alpha |
| spellingShingle |
Bone marrow fibroblast Lung carcinoma Prostaglandin E2 Tumor necrosis factor-α prostaglandin e2 tumor necrosis factor advanced cancer article bone marrow culture colony forming unit f controlled study enzyme linked immunosorbent assay human human tissue lung non small cell cancer mononuclear cell priority journal radioimmunoassay Bone Marrow Cells Carcinoma, Non-Small-Cell Lung Case-Control Studies Colony-Forming Units Assay Culture Media, Conditioned Dinoprostone Female Fibroblasts Humans Lung Neoplasms Male Stem Cells Tumor Necrosis Factor-alpha Chasseing, N.A. Bordenave, R.H. Bullorsky, E.O. Diaz, N.B. Stemmelin, G.R. Rumi, L.S. Fibroblastic colony-forming units and levels of tumor necrosis factor and prostaglandin E2 in bone marrow cultures from patients with advanced lung carcinoma |
| topic_facet |
Bone marrow fibroblast Lung carcinoma Prostaglandin E2 Tumor necrosis factor-α prostaglandin e2 tumor necrosis factor advanced cancer article bone marrow culture colony forming unit f controlled study enzyme linked immunosorbent assay human human tissue lung non small cell cancer mononuclear cell priority journal radioimmunoassay Bone Marrow Cells Carcinoma, Non-Small-Cell Lung Case-Control Studies Colony-Forming Units Assay Culture Media, Conditioned Dinoprostone Female Fibroblasts Humans Lung Neoplasms Male Stem Cells Tumor Necrosis Factor-alpha |
| description |
BACKGROUND. Although alterations of the bone marrow (BM) fibroblast colony-forming cells are involved in the development of diverse hematologic disorders, these progenitors still have not been well characterized in patients with solid tumors. METHODS. The incidence of fibroblast colony- forming units (CFU-F) was evaluated in the cultures of unseparated and fractionated light density BM mononuclear cells (MC) from 25 consecutive untreated lung carcinoma patients (LCP) and 16 normal controls (NC). Unseparated MC also were cultured in the presence of indomethacin (10-6 M). Finally, the authors evaluated the spontaneous production of prostaglandin E2 (PGE2) and tumor necrosis factor-α (TNF-α) in culture conditioned mediums of unseparated MC by radioimmunoassay and enzyme-linked immunoadsorbent assay methodology, respectively. RESULTS. A decreased number of CFU-F was observed in unseparated and fractionated (adherent and nonadherent) light density MC cultures from LCP compared with NC. When unseparated MC of LCP were treated with indomethacin, a slightly increase in the number of CFU-F was found. Adherent MC (stromal cells) achieved confluence only in 44% of LCP primary cultures compared with 100% of NC. Overproduction of PGE2 and TNF-α was found in the conditioned mediums of LCP compared with the mean values obtained in NC (P < 0.05 and P < 0.02, respectively). CONCLUSIONS. The lack of confluence and suppression of CFU-F in BM of LCP may be related to the increase production of PGE2 and TNF-α. Future investigation will allow the determination of how these modifications influence tumor cell growth and will prove if more alterations of the hematopoietic microenvironment imply a worse prognosis. |
| format |
JOUR |
| author |
Chasseing, N.A. Bordenave, R.H. Bullorsky, E.O. Diaz, N.B. Stemmelin, G.R. Rumi, L.S. |
| author_facet |
Chasseing, N.A. Bordenave, R.H. Bullorsky, E.O. Diaz, N.B. Stemmelin, G.R. Rumi, L.S. |
| author_sort |
Chasseing, N.A. |
| title |
Fibroblastic colony-forming units and levels of tumor necrosis factor and prostaglandin E2 in bone marrow cultures from patients with advanced lung carcinoma |
| title_short |
Fibroblastic colony-forming units and levels of tumor necrosis factor and prostaglandin E2 in bone marrow cultures from patients with advanced lung carcinoma |
| title_full |
Fibroblastic colony-forming units and levels of tumor necrosis factor and prostaglandin E2 in bone marrow cultures from patients with advanced lung carcinoma |
| title_fullStr |
Fibroblastic colony-forming units and levels of tumor necrosis factor and prostaglandin E2 in bone marrow cultures from patients with advanced lung carcinoma |
| title_full_unstemmed |
Fibroblastic colony-forming units and levels of tumor necrosis factor and prostaglandin E2 in bone marrow cultures from patients with advanced lung carcinoma |
| title_sort |
fibroblastic colony-forming units and levels of tumor necrosis factor and prostaglandin e2 in bone marrow cultures from patients with advanced lung carcinoma |
| url |
http://hdl.handle.net/20.500.12110/paper_0008543X_v80_n10_p1914_Chasseing |
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