Decreased Tamm-Horsfall protein in lithiasic patients

Objectives: Tamm-Horsfall glycoprotein (THP) is the most abundant substance of renal origin appearing in urine. It seems to be one of the major inhibitors of calcium oxalate crystal nucleation and/or aggregation, but its role in the pathogenesis of stone formation has not yet been clarified. The pre...

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Autores principales:	Romero, M.C., Nocera, S., Nesse, A.B.
Formato:	JOUR
Materias:	ELISA lithiasic patients stone formers Tamm-Horsfall glycoprotein urolithiasis uromodulin tamm horsfall glycoprotein article enzyme linked immunosorbent assay human priority journal protein determination protein urine level risk assessment stone formation Adult Aged Animals Enzyme-Linked Immunosorbent Assay Female Humans Kidney Calculi Male Middle Aged Mucoproteins Rabbits
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00099120_v30_n1_p63_Romero
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00099120_v30_n1_p63_Romero
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spelling	todo:paper_00099120_v30_n1_p63_Romero2023-10-03T14:08:50Z Decreased Tamm-Horsfall protein in lithiasic patients Romero, M.C. Nocera, S. Nesse, A.B. ELISA lithiasic patients stone formers Tamm-Horsfall glycoprotein urolithiasis uromodulin tamm horsfall glycoprotein article enzyme linked immunosorbent assay human priority journal protein determination protein urine level risk assessment stone formation urolithiasis Adult Aged Animals Enzyme-Linked Immunosorbent Assay Female Humans Kidney Calculi Male Middle Aged Mucoproteins Rabbits Objectives: Tamm-Horsfall glycoprotein (THP) is the most abundant substance of renal origin appearing in urine. It seems to be one of the major inhibitors of calcium oxalate crystal nucleation and/or aggregation, but its role in the pathogenesis of stone formation has not yet been clarified. The present work was undertaken to quantify THP excretion in lithiasic (L) patients, so as to determine if these levels are different from those of control subjects (C). Design and Methods: THP was isolated from human urine by reprecipitation steps, rabbit antiTHP antibody was obtained and its specificity determined, an ELISA was developed and technical conditions standardized, and quantitative measurements of urinary THP were performed on samples from L patients, who had suffered more than one lithiasic episode, and from C subjects. Microtiter plates coated with THP or diluted urine samples were subjected to successive incubation with antiTHP and alkaline phosphatase antirabbit IgG. Results: A good correlation between measured absorbance and THP concentration in standard and urine samples was observed. Data, expressed as Median and Interquartile Range, are 388/209626 μg THP/mmol creatinine for C (n = 85) and 124/82-171 μg THP/mmol creatinine for L (n = 23). Conclusions: We have obtained an antiserum antiTHP that can be used in the ELISA technique to determine reliable urinary THP values. The results show a significant decrease of THP excretion in recurrent stone formers compared to controls (p < 0.001) and may have interesting implications in the pathogenesis of urolithiasis. Fil:Nesse, A.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00099120_v30_n1_p63_Romero
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	ELISA lithiasic patients stone formers Tamm-Horsfall glycoprotein urolithiasis uromodulin tamm horsfall glycoprotein article enzyme linked immunosorbent assay human priority journal protein determination protein urine level risk assessment stone formation urolithiasis Adult Aged Animals Enzyme-Linked Immunosorbent Assay Female Humans Kidney Calculi Male Middle Aged Mucoproteins Rabbits
spellingShingle	ELISA lithiasic patients stone formers Tamm-Horsfall glycoprotein urolithiasis uromodulin tamm horsfall glycoprotein article enzyme linked immunosorbent assay human priority journal protein determination protein urine level risk assessment stone formation urolithiasis Adult Aged Animals Enzyme-Linked Immunosorbent Assay Female Humans Kidney Calculi Male Middle Aged Mucoproteins Rabbits Romero, M.C. Nocera, S. Nesse, A.B. Decreased Tamm-Horsfall protein in lithiasic patients
topic_facet	ELISA lithiasic patients stone formers Tamm-Horsfall glycoprotein urolithiasis uromodulin tamm horsfall glycoprotein article enzyme linked immunosorbent assay human priority journal protein determination protein urine level risk assessment stone formation urolithiasis Adult Aged Animals Enzyme-Linked Immunosorbent Assay Female Humans Kidney Calculi Male Middle Aged Mucoproteins Rabbits
description	Objectives: Tamm-Horsfall glycoprotein (THP) is the most abundant substance of renal origin appearing in urine. It seems to be one of the major inhibitors of calcium oxalate crystal nucleation and/or aggregation, but its role in the pathogenesis of stone formation has not yet been clarified. The present work was undertaken to quantify THP excretion in lithiasic (L) patients, so as to determine if these levels are different from those of control subjects (C). Design and Methods: THP was isolated from human urine by reprecipitation steps, rabbit antiTHP antibody was obtained and its specificity determined, an ELISA was developed and technical conditions standardized, and quantitative measurements of urinary THP were performed on samples from L patients, who had suffered more than one lithiasic episode, and from C subjects. Microtiter plates coated with THP or diluted urine samples were subjected to successive incubation with antiTHP and alkaline phosphatase antirabbit IgG. Results: A good correlation between measured absorbance and THP concentration in standard and urine samples was observed. Data, expressed as Median and Interquartile Range, are 388/209626 μg THP/mmol creatinine for C (n = 85) and 124/82-171 μg THP/mmol creatinine for L (n = 23). Conclusions: We have obtained an antiserum antiTHP that can be used in the ELISA technique to determine reliable urinary THP values. The results show a significant decrease of THP excretion in recurrent stone formers compared to controls (p < 0.001) and may have interesting implications in the pathogenesis of urolithiasis.
format	JOUR
author	Romero, M.C. Nocera, S. Nesse, A.B.
author_facet	Romero, M.C. Nocera, S. Nesse, A.B.
author_sort	Romero, M.C.
title	Decreased Tamm-Horsfall protein in lithiasic patients
title_short	Decreased Tamm-Horsfall protein in lithiasic patients
title_full	Decreased Tamm-Horsfall protein in lithiasic patients
title_fullStr	Decreased Tamm-Horsfall protein in lithiasic patients
title_full_unstemmed	Decreased Tamm-Horsfall protein in lithiasic patients
title_sort	decreased tamm-horsfall protein in lithiasic patients
url	http://hdl.handle.net/20.500.12110/paper_00099120_v30_n1_p63_Romero
work_keys_str_mv	AT romeromc decreasedtammhorsfallproteininlithiasicpatients AT noceras decreasedtammhorsfallproteininlithiasicpatients AT nesseab decreasedtammhorsfallproteininlithiasicpatients
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Decreased Tamm-Horsfall protein in lithiasic patients

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