Dipteran chromosomes: A simple method for obtaining high quality chromosomal preparations

For cytogenetic studies it is necessary to develop an efficient method of chromosome preparation with wellpreserved and dispersed chromosomes and little or no cell-wall debris. In this study, we describe a method for obtaining high quality chromosomal preparations of dipteran cells. This procedure c...

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Detalles Bibliográficos
Autores principales:	Chirino, M.G., Rossi, L.F., Bressa, M.J., Luaces, J.P., Merani, M.S.
Formato:	JOUR
Materias:	Calliphoridae Diptera
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00113891_v107_n11_p1792_Chirino
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00113891_v107_n11_p1792_Chirino
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spelling	todo:paper_00113891_v107_n11_p1792_Chirino2023-10-03T14:09:44Z Dipteran chromosomes: A simple method for obtaining high quality chromosomal preparations Chirino, M.G. Rossi, L.F. Bressa, M.J. Luaces, J.P. Merani, M.S. Calliphoridae Diptera For cytogenetic studies it is necessary to develop an efficient method of chromosome preparation with wellpreserved and dispersed chromosomes and little or no cell-wall debris. In this study, we describe a method for obtaining high quality chromosomal preparations of dipteran cells. This procedure combines drop method and air-dry technique and produces a cell suspension with separated and mixed cells. Suspended mitotic cells were then dropped onto a glass slide in order to distribute the cytoplasm and spread the chromosomes. We compare and evaluate different chromosome preparation methods to develop a more reliable procedure for the resolution of chromosome characteristics and chromosome bandings in blow fly mitotic chromosomes. The combined drop and air-dry technique described here is convenient for identification of sex chromosomes. Using this protocol, acceptable high-quality chromosomal spreads with flattened cells and no cell debris, and without damage and/or loss of chromosomes were obtained. Fil:Rossi, L.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bressa, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Luaces, J.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00113891_v107_n11_p1792_Chirino
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Calliphoridae Diptera
spellingShingle	Calliphoridae Diptera Chirino, M.G. Rossi, L.F. Bressa, M.J. Luaces, J.P. Merani, M.S. Dipteran chromosomes: A simple method for obtaining high quality chromosomal preparations
topic_facet	Calliphoridae Diptera
description	For cytogenetic studies it is necessary to develop an efficient method of chromosome preparation with wellpreserved and dispersed chromosomes and little or no cell-wall debris. In this study, we describe a method for obtaining high quality chromosomal preparations of dipteran cells. This procedure combines drop method and air-dry technique and produces a cell suspension with separated and mixed cells. Suspended mitotic cells were then dropped onto a glass slide in order to distribute the cytoplasm and spread the chromosomes. We compare and evaluate different chromosome preparation methods to develop a more reliable procedure for the resolution of chromosome characteristics and chromosome bandings in blow fly mitotic chromosomes. The combined drop and air-dry technique described here is convenient for identification of sex chromosomes. Using this protocol, acceptable high-quality chromosomal spreads with flattened cells and no cell debris, and without damage and/or loss of chromosomes were obtained.
format	JOUR
author	Chirino, M.G. Rossi, L.F. Bressa, M.J. Luaces, J.P. Merani, M.S.
author_facet	Chirino, M.G. Rossi, L.F. Bressa, M.J. Luaces, J.P. Merani, M.S.
author_sort	Chirino, M.G.
title	Dipteran chromosomes: A simple method for obtaining high quality chromosomal preparations
title_short	Dipteran chromosomes: A simple method for obtaining high quality chromosomal preparations
title_full	Dipteran chromosomes: A simple method for obtaining high quality chromosomal preparations
title_fullStr	Dipteran chromosomes: A simple method for obtaining high quality chromosomal preparations
title_full_unstemmed	Dipteran chromosomes: A simple method for obtaining high quality chromosomal preparations
title_sort	dipteran chromosomes: a simple method for obtaining high quality chromosomal preparations
url	http://hdl.handle.net/20.500.12110/paper_00113891_v107_n11_p1792_Chirino
work_keys_str_mv	AT chirinomg dipteranchromosomesasimplemethodforobtaininghighqualitychromosomalpreparations AT rossilf dipteranchromosomesasimplemethodforobtaininghighqualitychromosomalpreparations AT bressamj dipteranchromosomesasimplemethodforobtaininghighqualitychromosomalpreparations AT luacesjp dipteranchromosomesasimplemethodforobtaininghighqualitychromosomalpreparations AT meranims dipteranchromosomesasimplemethodforobtaininghighqualitychromosomalpreparations
_version_	1807314521536069632

Dipteran chromosomes: A simple method for obtaining high quality chromosomal preparations

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