Prostaglandin F2α decreases the affinity of epidermal growth factor receptors in Swiss mouse 3T3 cells via protein kinase C activation
Prostaglandin F2α (PGF2α) selectively decreases the binding of 125I-labelled epidermal growth factor ([125I]EGF) to intact Swiss 3T3 cells. Scatchard analysis reveals that PGF2α decreases the number of high-affinity EGF binding sites without changing the total number of receptors. Prostaglandins E1...
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todo:paper_00145793_v299_n3_p235_deAsua2023-10-03T14:13:01Z Prostaglandin F2α decreases the affinity of epidermal growth factor receptors in Swiss mouse 3T3 cells via protein kinase C activation de Asua, L.J. Goin, M. Diacylglycerol DNA synthesis Epidermal growth factor receptor Prostaglandin F2α Protein kinase C Swiss 3T3 cells 3 [2 [4 (4,4' difluorobenzhydrylidene)piperidino]ethyl] 2,3 dihydro 2 thioxo 4(1h) quinazolinone 6 [2 [4 [(4 fluorophenyl)phenylmethylene] 1 piperidyl]ethyl] 7 methyl 5h thiazolo[3,2 a]pyrimidin 5 one enzyme inhibitor epidermal growth factor epidermal growth factor receptor prostaglandin derivative prostaglandin f2 alpha protein kinase c diacylglycerol kinase epidermal growth factor epidermal growth factor receptor phosphotransferase prostaglandin E prostaglandin F2 alpha protein kinase C animal cell article binding affinity enzyme activation mouse nonhuman priority journal receptor binding animal cell strain 3T3 dose response drug effect enzyme activation metabolism sensitivity and specificity 3T3 Cells Animal Diacylglycerol Kinase Dinoprost Dose-Response Relationship, Drug Enzyme Activation Epidermal Growth Factor Mice Phosphotransferases Prostaglandins E Protein Kinase C Receptor, Epidermal Growth Factor Sensitivity and Specificity Support, Non-U.S. Gov't Prostaglandin F2α (PGF2α) selectively decreases the binding of 125I-labelled epidermal growth factor ([125I]EGF) to intact Swiss 3T3 cells. Scatchard analysis reveals that PGF2α decreases the number of high-affinity EGF binding sites without changing the total number of receptors. Prostaglandins E1 (PGE1), E2 (PGE2 or F2β (PGF2β) do not alter the EGF binding to these cells and do not enhance the PGF2α effect. R-59022 and R-59949, two diacylglycerol kinase inhibitors, enhance the inhibitory effect of PGF2α, whereas down-modulation of protein kinase C (PKC) abolishes the effect. These results indicate that PGF2α, decreases EGF binding in Swiss 3T3 cells via PKC activation. © 1992. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00145793_v299_n3_p235_deAsua |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Diacylglycerol DNA synthesis Epidermal growth factor receptor Prostaglandin F2α Protein kinase C Swiss 3T3 cells 3 [2 [4 (4,4' difluorobenzhydrylidene)piperidino]ethyl] 2,3 dihydro 2 thioxo 4(1h) quinazolinone 6 [2 [4 [(4 fluorophenyl)phenylmethylene] 1 piperidyl]ethyl] 7 methyl 5h thiazolo[3,2 a]pyrimidin 5 one enzyme inhibitor epidermal growth factor epidermal growth factor receptor prostaglandin derivative prostaglandin f2 alpha protein kinase c diacylglycerol kinase epidermal growth factor epidermal growth factor receptor phosphotransferase prostaglandin E prostaglandin F2 alpha protein kinase C animal cell article binding affinity enzyme activation mouse nonhuman priority journal receptor binding animal cell strain 3T3 dose response drug effect enzyme activation metabolism sensitivity and specificity 3T3 Cells Animal Diacylglycerol Kinase Dinoprost Dose-Response Relationship, Drug Enzyme Activation Epidermal Growth Factor Mice Phosphotransferases Prostaglandins E Protein Kinase C Receptor, Epidermal Growth Factor Sensitivity and Specificity Support, Non-U.S. Gov't |
| spellingShingle |
Diacylglycerol DNA synthesis Epidermal growth factor receptor Prostaglandin F2α Protein kinase C Swiss 3T3 cells 3 [2 [4 (4,4' difluorobenzhydrylidene)piperidino]ethyl] 2,3 dihydro 2 thioxo 4(1h) quinazolinone 6 [2 [4 [(4 fluorophenyl)phenylmethylene] 1 piperidyl]ethyl] 7 methyl 5h thiazolo[3,2 a]pyrimidin 5 one enzyme inhibitor epidermal growth factor epidermal growth factor receptor prostaglandin derivative prostaglandin f2 alpha protein kinase c diacylglycerol kinase epidermal growth factor epidermal growth factor receptor phosphotransferase prostaglandin E prostaglandin F2 alpha protein kinase C animal cell article binding affinity enzyme activation mouse nonhuman priority journal receptor binding animal cell strain 3T3 dose response drug effect enzyme activation metabolism sensitivity and specificity 3T3 Cells Animal Diacylglycerol Kinase Dinoprost Dose-Response Relationship, Drug Enzyme Activation Epidermal Growth Factor Mice Phosphotransferases Prostaglandins E Protein Kinase C Receptor, Epidermal Growth Factor Sensitivity and Specificity Support, Non-U.S. Gov't de Asua, L.J. Goin, M. Prostaglandin F2α decreases the affinity of epidermal growth factor receptors in Swiss mouse 3T3 cells via protein kinase C activation |
| topic_facet |
Diacylglycerol DNA synthesis Epidermal growth factor receptor Prostaglandin F2α Protein kinase C Swiss 3T3 cells 3 [2 [4 (4,4' difluorobenzhydrylidene)piperidino]ethyl] 2,3 dihydro 2 thioxo 4(1h) quinazolinone 6 [2 [4 [(4 fluorophenyl)phenylmethylene] 1 piperidyl]ethyl] 7 methyl 5h thiazolo[3,2 a]pyrimidin 5 one enzyme inhibitor epidermal growth factor epidermal growth factor receptor prostaglandin derivative prostaglandin f2 alpha protein kinase c diacylglycerol kinase epidermal growth factor epidermal growth factor receptor phosphotransferase prostaglandin E prostaglandin F2 alpha protein kinase C animal cell article binding affinity enzyme activation mouse nonhuman priority journal receptor binding animal cell strain 3T3 dose response drug effect enzyme activation metabolism sensitivity and specificity 3T3 Cells Animal Diacylglycerol Kinase Dinoprost Dose-Response Relationship, Drug Enzyme Activation Epidermal Growth Factor Mice Phosphotransferases Prostaglandins E Protein Kinase C Receptor, Epidermal Growth Factor Sensitivity and Specificity Support, Non-U.S. Gov't |
| description |
Prostaglandin F2α (PGF2α) selectively decreases the binding of 125I-labelled epidermal growth factor ([125I]EGF) to intact Swiss 3T3 cells. Scatchard analysis reveals that PGF2α decreases the number of high-affinity EGF binding sites without changing the total number of receptors. Prostaglandins E1 (PGE1), E2 (PGE2 or F2β (PGF2β) do not alter the EGF binding to these cells and do not enhance the PGF2α effect. R-59022 and R-59949, two diacylglycerol kinase inhibitors, enhance the inhibitory effect of PGF2α, whereas down-modulation of protein kinase C (PKC) abolishes the effect. These results indicate that PGF2α, decreases EGF binding in Swiss 3T3 cells via PKC activation. © 1992. |
| format |
JOUR |
| author |
de Asua, L.J. Goin, M. |
| author_facet |
de Asua, L.J. Goin, M. |
| author_sort |
de Asua, L.J. |
| title |
Prostaglandin F2α decreases the affinity of epidermal growth factor receptors in Swiss mouse 3T3 cells via protein kinase C activation |
| title_short |
Prostaglandin F2α decreases the affinity of epidermal growth factor receptors in Swiss mouse 3T3 cells via protein kinase C activation |
| title_full |
Prostaglandin F2α decreases the affinity of epidermal growth factor receptors in Swiss mouse 3T3 cells via protein kinase C activation |
| title_fullStr |
Prostaglandin F2α decreases the affinity of epidermal growth factor receptors in Swiss mouse 3T3 cells via protein kinase C activation |
| title_full_unstemmed |
Prostaglandin F2α decreases the affinity of epidermal growth factor receptors in Swiss mouse 3T3 cells via protein kinase C activation |
| title_sort |
prostaglandin f2α decreases the affinity of epidermal growth factor receptors in swiss mouse 3t3 cells via protein kinase c activation |
| url |
http://hdl.handle.net/20.500.12110/paper_00145793_v299_n3_p235_deAsua |
| work_keys_str_mv |
AT deasualj prostaglandinf2adecreasestheaffinityofepidermalgrowthfactorreceptorsinswissmouse3t3cellsviaproteinkinasecactivation AT goinm prostaglandinf2adecreasestheaffinityofepidermalgrowthfactorreceptorsinswissmouse3t3cellsviaproteinkinasecactivation |
| _version_ |
1807322984224915456 |