A game of two? Gene expression analysis of brain (cyp19a1b) and gonadal (cyp19a1a) aromatase in females of a Neotropical cichlid fish through the parental care period and removal of the offspring
For many species parental behavior is essential for the survival of the offspring. While the ultimate causes of teleost parental behavior have been widely studied, comparatively little is known about its proximate causes. The aim of this study was to analyze the yet unexplored, potential dual role o...
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todo:paper_00166480_v252_n_p119_Ramallo2023-10-03T14:14:06Z A game of two? Gene expression analysis of brain (cyp19a1b) and gonadal (cyp19a1a) aromatase in females of a Neotropical cichlid fish through the parental care period and removal of the offspring Ramallo, M.R. Honji, R.M. Birba, A. Morandini, L. Varela, M.L. Genovese, G. Moreira, R.G. Somoza, G.M. Pandolfi, M. Brain aromatase Cichlasoma dimerus Cichlids Gonadal aromatase Maternal care Offspring removal Ovary Sex steroids 11 ketotestoterone estradiol testosterone unclassified drug aromatase estradiol sex hormone testosterone adult animal cell animal experiment Article Cichlasoma dimerus cichlid controlled study cyp19a1a gene cyp19a1b gene down regulation estradiol blood level female forebrain gene gene expression immunohistochemistry male maternal care nonhuman ovary ovary development ovary follicle ovary follicle development parental behavior priority journal progeny steroidogenesis testosterone blood level anatomy and histology animal animal behavior blood brain enzymology fluorescence gene expression regulation genetics metabolism reproduction tropic climate Animals Aromatase Behavior, Animal Brain Cichlids Estradiol Female Fluorescence Gene Expression Regulation, Enzymologic Gonadal Steroid Hormones Male Ovary Reproduction Testosterone Tropical Climate For many species parental behavior is essential for the survival of the offspring. While the ultimate causes of teleost parental behavior have been widely studied, comparatively little is known about its proximate causes. The aim of this study was to analyze the yet unexplored, potential dual role of brain and gonadal aromatases, the enzymes responsible for the conversion of androgens to estrogens in the brains and gonads of teleosts, respectively, on the different stages of the maternal care period of the biparental cichlid Cichlasoma dimerus, locally known as chanchita. By immunohistochemistry we analyzed the neural distribution of brain aromatase and observed it exclusively within the forebrain, including areas involved in the regulation of parental behavior. We next analyzed the gene expression of brain aromatase in the brain, and gonadal aromatase in the ovary, of female chanchitas through the parental care period. To further characterize the physiological environment associated to maternal care, we also evaluated sex steroid levels (17β-estradiol, testosterone and 11-ketotestoterone) and ovarian follicle percentage. The onset of parental behavior specifically downregulated sex steroids synthesis and the rate of ovarian maturation, as denoted by a more than 10-fold decrease in steroid levels and delayed detection of mature follicles in females with offspring, compared to females which eggs were removed. Gene expression levels of both aromatases were independent of maternal care at the evaluated time points, even though they varied during the parental care period. © 2017 Elsevier Inc. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00166480_v252_n_p119_Ramallo |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Brain aromatase Cichlasoma dimerus Cichlids Gonadal aromatase Maternal care Offspring removal Ovary Sex steroids 11 ketotestoterone estradiol testosterone unclassified drug aromatase estradiol sex hormone testosterone adult animal cell animal experiment Article Cichlasoma dimerus cichlid controlled study cyp19a1a gene cyp19a1b gene down regulation estradiol blood level female forebrain gene gene expression immunohistochemistry male maternal care nonhuman ovary ovary development ovary follicle ovary follicle development parental behavior priority journal progeny steroidogenesis testosterone blood level anatomy and histology animal animal behavior blood brain enzymology fluorescence gene expression regulation genetics metabolism reproduction tropic climate Animals Aromatase Behavior, Animal Brain Cichlids Estradiol Female Fluorescence Gene Expression Regulation, Enzymologic Gonadal Steroid Hormones Male Ovary Reproduction Testosterone Tropical Climate |
| spellingShingle |
Brain aromatase Cichlasoma dimerus Cichlids Gonadal aromatase Maternal care Offspring removal Ovary Sex steroids 11 ketotestoterone estradiol testosterone unclassified drug aromatase estradiol sex hormone testosterone adult animal cell animal experiment Article Cichlasoma dimerus cichlid controlled study cyp19a1a gene cyp19a1b gene down regulation estradiol blood level female forebrain gene gene expression immunohistochemistry male maternal care nonhuman ovary ovary development ovary follicle ovary follicle development parental behavior priority journal progeny steroidogenesis testosterone blood level anatomy and histology animal animal behavior blood brain enzymology fluorescence gene expression regulation genetics metabolism reproduction tropic climate Animals Aromatase Behavior, Animal Brain Cichlids Estradiol Female Fluorescence Gene Expression Regulation, Enzymologic Gonadal Steroid Hormones Male Ovary Reproduction Testosterone Tropical Climate Ramallo, M.R. Honji, R.M. Birba, A. Morandini, L. Varela, M.L. Genovese, G. Moreira, R.G. Somoza, G.M. Pandolfi, M. A game of two? Gene expression analysis of brain (cyp19a1b) and gonadal (cyp19a1a) aromatase in females of a Neotropical cichlid fish through the parental care period and removal of the offspring |
| topic_facet |
Brain aromatase Cichlasoma dimerus Cichlids Gonadal aromatase Maternal care Offspring removal Ovary Sex steroids 11 ketotestoterone estradiol testosterone unclassified drug aromatase estradiol sex hormone testosterone adult animal cell animal experiment Article Cichlasoma dimerus cichlid controlled study cyp19a1a gene cyp19a1b gene down regulation estradiol blood level female forebrain gene gene expression immunohistochemistry male maternal care nonhuman ovary ovary development ovary follicle ovary follicle development parental behavior priority journal progeny steroidogenesis testosterone blood level anatomy and histology animal animal behavior blood brain enzymology fluorescence gene expression regulation genetics metabolism reproduction tropic climate Animals Aromatase Behavior, Animal Brain Cichlids Estradiol Female Fluorescence Gene Expression Regulation, Enzymologic Gonadal Steroid Hormones Male Ovary Reproduction Testosterone Tropical Climate |
| description |
For many species parental behavior is essential for the survival of the offspring. While the ultimate causes of teleost parental behavior have been widely studied, comparatively little is known about its proximate causes. The aim of this study was to analyze the yet unexplored, potential dual role of brain and gonadal aromatases, the enzymes responsible for the conversion of androgens to estrogens in the brains and gonads of teleosts, respectively, on the different stages of the maternal care period of the biparental cichlid Cichlasoma dimerus, locally known as chanchita. By immunohistochemistry we analyzed the neural distribution of brain aromatase and observed it exclusively within the forebrain, including areas involved in the regulation of parental behavior. We next analyzed the gene expression of brain aromatase in the brain, and gonadal aromatase in the ovary, of female chanchitas through the parental care period. To further characterize the physiological environment associated to maternal care, we also evaluated sex steroid levels (17β-estradiol, testosterone and 11-ketotestoterone) and ovarian follicle percentage. The onset of parental behavior specifically downregulated sex steroids synthesis and the rate of ovarian maturation, as denoted by a more than 10-fold decrease in steroid levels and delayed detection of mature follicles in females with offspring, compared to females which eggs were removed. Gene expression levels of both aromatases were independent of maternal care at the evaluated time points, even though they varied during the parental care period. © 2017 Elsevier Inc. |
| format |
JOUR |
| author |
Ramallo, M.R. Honji, R.M. Birba, A. Morandini, L. Varela, M.L. Genovese, G. Moreira, R.G. Somoza, G.M. Pandolfi, M. |
| author_facet |
Ramallo, M.R. Honji, R.M. Birba, A. Morandini, L. Varela, M.L. Genovese, G. Moreira, R.G. Somoza, G.M. Pandolfi, M. |
| author_sort |
Ramallo, M.R. |
| title |
A game of two? Gene expression analysis of brain (cyp19a1b) and gonadal (cyp19a1a) aromatase in females of a Neotropical cichlid fish through the parental care period and removal of the offspring |
| title_short |
A game of two? Gene expression analysis of brain (cyp19a1b) and gonadal (cyp19a1a) aromatase in females of a Neotropical cichlid fish through the parental care period and removal of the offspring |
| title_full |
A game of two? Gene expression analysis of brain (cyp19a1b) and gonadal (cyp19a1a) aromatase in females of a Neotropical cichlid fish through the parental care period and removal of the offspring |
| title_fullStr |
A game of two? Gene expression analysis of brain (cyp19a1b) and gonadal (cyp19a1a) aromatase in females of a Neotropical cichlid fish through the parental care period and removal of the offspring |
| title_full_unstemmed |
A game of two? Gene expression analysis of brain (cyp19a1b) and gonadal (cyp19a1a) aromatase in females of a Neotropical cichlid fish through the parental care period and removal of the offspring |
| title_sort |
game of two? gene expression analysis of brain (cyp19a1b) and gonadal (cyp19a1a) aromatase in females of a neotropical cichlid fish through the parental care period and removal of the offspring |
| url |
http://hdl.handle.net/20.500.12110/paper_00166480_v252_n_p119_Ramallo |
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