Fibronectin rescues estrogen receptor α from lysosomal degradation in breast cancer cells

Estrogen receptor α (ERα) is expressed in tissues as diverse as brains and mammary glands. In breast cancer, ERα is a key regulator of tumor progression. Therefore, understanding what activates ERα is critical for cancer treatment in particular and cell biology in general. Using biochemical approach...

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Autores principales:	Sampayo, R.G., Toscani, A.M., Rubashkin, M.G., Thi, K., Masullo, L.A., Violi, I.L., Lakins, J.N., Cáceres, A., Hines, W.C., Leskow, F.C., Stefani, F.D., Chialvo, D.R., Bissell, M.J., Weaver, V.M., Simian, M.
Formato:	JOUR
Materias:	beta1 integrin estrogen estrogen receptor alpha fibronectin Article biochemical analysis breast cancer breast tissue cancer cell cell compartmentalization cell fate cell vacuole controlled study endocytosis endosome estrogen activity extracellular matrix human human cell human tissue intracellular transport lysosome microscopy priority journal protein degradation protein function protein localization protein protein interaction signal transduction
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00219525_v217_n8_p2777_Sampayo
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00219525_v217_n8_p2777_Sampayo
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spelling	todo:paper_00219525_v217_n8_p2777_Sampayo2023-10-03T14:23:29Z Fibronectin rescues estrogen receptor α from lysosomal degradation in breast cancer cells Sampayo, R.G. Toscani, A.M. Rubashkin, M.G. Thi, K. Masullo, L.A. Violi, I.L. Lakins, J.N. Cáceres, A. Hines, W.C. Leskow, F.C. Stefani, F.D. Chialvo, D.R. Bissell, M.J. Weaver, V.M. Simian, M. beta1 integrin estrogen estrogen receptor alpha fibronectin Article biochemical analysis breast cancer breast tissue cancer cell cell compartmentalization cell fate cell vacuole controlled study endocytosis endosome estrogen activity extracellular matrix human human cell human tissue intracellular transport lysosome microscopy priority journal protein degradation protein function protein localization protein protein interaction signal transduction Estrogen receptor α (ERα) is expressed in tissues as diverse as brains and mammary glands. In breast cancer, ERα is a key regulator of tumor progression. Therefore, understanding what activates ERα is critical for cancer treatment in particular and cell biology in general. Using biochemical approaches and superresolution microscopy, we show that estrogen drives membrane ERα into endosomes in breast cancer cells and that its fate is determined by the presence of fibronectin (FN) in the extracellular matrix; it is trafficked to lysosomes in the absence of FN and avoids the lysosomal compartment in its presence. In this context, FN prolongs ERα half-life and strengthens its transcriptional activity. We show that ERα is associated with β1-integrin at the membrane, and this integrin follows the same endocytosis and subcellular trafficking pathway triggered by estrogen. Moreover, ERα+ vesicles are present within human breast tissues, and colocalization with β1-integrin is detected primarily in tumors. Our work unravels a key, clinically relevant mechanism of microenvironmental regulation of ERα signaling. © 2018 Sampayo et al. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00219525_v217_n8_p2777_Sampayo
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	beta1 integrin estrogen estrogen receptor alpha fibronectin Article biochemical analysis breast cancer breast tissue cancer cell cell compartmentalization cell fate cell vacuole controlled study endocytosis endosome estrogen activity extracellular matrix human human cell human tissue intracellular transport lysosome microscopy priority journal protein degradation protein function protein localization protein protein interaction signal transduction
spellingShingle	beta1 integrin estrogen estrogen receptor alpha fibronectin Article biochemical analysis breast cancer breast tissue cancer cell cell compartmentalization cell fate cell vacuole controlled study endocytosis endosome estrogen activity extracellular matrix human human cell human tissue intracellular transport lysosome microscopy priority journal protein degradation protein function protein localization protein protein interaction signal transduction Sampayo, R.G. Toscani, A.M. Rubashkin, M.G. Thi, K. Masullo, L.A. Violi, I.L. Lakins, J.N. Cáceres, A. Hines, W.C. Leskow, F.C. Stefani, F.D. Chialvo, D.R. Bissell, M.J. Weaver, V.M. Simian, M. Fibronectin rescues estrogen receptor α from lysosomal degradation in breast cancer cells
topic_facet	beta1 integrin estrogen estrogen receptor alpha fibronectin Article biochemical analysis breast cancer breast tissue cancer cell cell compartmentalization cell fate cell vacuole controlled study endocytosis endosome estrogen activity extracellular matrix human human cell human tissue intracellular transport lysosome microscopy priority journal protein degradation protein function protein localization protein protein interaction signal transduction
description	Estrogen receptor α (ERα) is expressed in tissues as diverse as brains and mammary glands. In breast cancer, ERα is a key regulator of tumor progression. Therefore, understanding what activates ERα is critical for cancer treatment in particular and cell biology in general. Using biochemical approaches and superresolution microscopy, we show that estrogen drives membrane ERα into endosomes in breast cancer cells and that its fate is determined by the presence of fibronectin (FN) in the extracellular matrix; it is trafficked to lysosomes in the absence of FN and avoids the lysosomal compartment in its presence. In this context, FN prolongs ERα half-life and strengthens its transcriptional activity. We show that ERα is associated with β1-integrin at the membrane, and this integrin follows the same endocytosis and subcellular trafficking pathway triggered by estrogen. Moreover, ERα+ vesicles are present within human breast tissues, and colocalization with β1-integrin is detected primarily in tumors. Our work unravels a key, clinically relevant mechanism of microenvironmental regulation of ERα signaling. © 2018 Sampayo et al.
format	JOUR
author	Sampayo, R.G. Toscani, A.M. Rubashkin, M.G. Thi, K. Masullo, L.A. Violi, I.L. Lakins, J.N. Cáceres, A. Hines, W.C. Leskow, F.C. Stefani, F.D. Chialvo, D.R. Bissell, M.J. Weaver, V.M. Simian, M.
author_facet	Sampayo, R.G. Toscani, A.M. Rubashkin, M.G. Thi, K. Masullo, L.A. Violi, I.L. Lakins, J.N. Cáceres, A. Hines, W.C. Leskow, F.C. Stefani, F.D. Chialvo, D.R. Bissell, M.J. Weaver, V.M. Simian, M.
author_sort	Sampayo, R.G.
title	Fibronectin rescues estrogen receptor α from lysosomal degradation in breast cancer cells
title_short	Fibronectin rescues estrogen receptor α from lysosomal degradation in breast cancer cells
title_full	Fibronectin rescues estrogen receptor α from lysosomal degradation in breast cancer cells
title_fullStr	Fibronectin rescues estrogen receptor α from lysosomal degradation in breast cancer cells
title_full_unstemmed	Fibronectin rescues estrogen receptor α from lysosomal degradation in breast cancer cells
title_sort	fibronectin rescues estrogen receptor α from lysosomal degradation in breast cancer cells
url	http://hdl.handle.net/20.500.12110/paper_00219525_v217_n8_p2777_Sampayo
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Fibronectin rescues estrogen receptor α from lysosomal degradation in breast cancer cells

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