Differential effect of αdifluoromethyl‐ornithine on the proliferation of balb 3T3 and chemically transformed 3T3 cells
In order to study the effect of polyamine depletion on growth and proliferation of untransformed and chemically transformed cells, α‐difluoromethyl‐ornithine (DFMO) was added to cultures of 3T3 cells and their benzo[a]pyrene derivative BP‐3T3. Both types of cells stopped their proliferation after 72...
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todo:paper_00219541_v117_n2_p141_Medrano2023-10-03T14:23:36Z Differential effect of αdifluoromethyl‐ornithine on the proliferation of balb 3T3 and chemically transformed 3T3 cells Medrano, E.E. Goldemberg, S.H. Algranati, I.D. benzo[a]pyrene eflornithine polyamine putrescine radioisotope spermidine animal cell cancer chemotherapy cell culture cell proliferation cell transformation drug therapy in vitro study mouse nonhuman reticuloendothelial system therapy thymidine h 3 Animal Benzo(a)pyrene Benzopyrenes Carcinogens Cell Division Cell Transformation, Neoplastic Cells, Cultured DNA Replication Eflornithine Interphase Kinetics Mice Mice, Inbred BALB C Ornithine Ornithine Decarboxylase Putrescine Support, Non-U.S. Gov't Transcription, Genetic Translation, Genetic In order to study the effect of polyamine depletion on growth and proliferation of untransformed and chemically transformed cells, α‐difluoromethyl‐ornithine (DFMO) was added to cultures of 3T3 cells and their benzo[a]pyrene derivative BP‐3T3. Both types of cells stopped their proliferation after 72 hr of treatment with the inhibitor. When DFMO was removed and cells were cultivated afterwards in fresh medium without the drug, untransformed cells resumed growth after a lag period, whereas transformed cells were unable to proliferate unless exogenous polyamine was added. These alterations showed a strict correlation with intracellular polyamine pools, since after removal of DFMO from the culture medium, polyamine concentrations increased to almost normal values in 3T3 cells, but remained at low levels or decreased even more in the transformed cells BP‐3T3. The analysis by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis of labeled proteins of both cell extracts has indicated that the described control of cell proliferation by intracellular polyamine levels might be related to the synthesis of at least two proteins with molecular weights of about 36,000 and 55,000 daltons. Copyright © 1983 Wiley‐Liss, Inc. Fil:Goldemberg, S.H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Algranati, I.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00219541_v117_n2_p141_Medrano |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
benzo[a]pyrene eflornithine polyamine putrescine radioisotope spermidine animal cell cancer chemotherapy cell culture cell proliferation cell transformation drug therapy in vitro study mouse nonhuman reticuloendothelial system therapy thymidine h 3 Animal Benzo(a)pyrene Benzopyrenes Carcinogens Cell Division Cell Transformation, Neoplastic Cells, Cultured DNA Replication Eflornithine Interphase Kinetics Mice Mice, Inbred BALB C Ornithine Ornithine Decarboxylase Putrescine Support, Non-U.S. Gov't Transcription, Genetic Translation, Genetic |
| spellingShingle |
benzo[a]pyrene eflornithine polyamine putrescine radioisotope spermidine animal cell cancer chemotherapy cell culture cell proliferation cell transformation drug therapy in vitro study mouse nonhuman reticuloendothelial system therapy thymidine h 3 Animal Benzo(a)pyrene Benzopyrenes Carcinogens Cell Division Cell Transformation, Neoplastic Cells, Cultured DNA Replication Eflornithine Interphase Kinetics Mice Mice, Inbred BALB C Ornithine Ornithine Decarboxylase Putrescine Support, Non-U.S. Gov't Transcription, Genetic Translation, Genetic Medrano, E.E. Goldemberg, S.H. Algranati, I.D. Differential effect of αdifluoromethyl‐ornithine on the proliferation of balb 3T3 and chemically transformed 3T3 cells |
| topic_facet |
benzo[a]pyrene eflornithine polyamine putrescine radioisotope spermidine animal cell cancer chemotherapy cell culture cell proliferation cell transformation drug therapy in vitro study mouse nonhuman reticuloendothelial system therapy thymidine h 3 Animal Benzo(a)pyrene Benzopyrenes Carcinogens Cell Division Cell Transformation, Neoplastic Cells, Cultured DNA Replication Eflornithine Interphase Kinetics Mice Mice, Inbred BALB C Ornithine Ornithine Decarboxylase Putrescine Support, Non-U.S. Gov't Transcription, Genetic Translation, Genetic |
| description |
In order to study the effect of polyamine depletion on growth and proliferation of untransformed and chemically transformed cells, α‐difluoromethyl‐ornithine (DFMO) was added to cultures of 3T3 cells and their benzo[a]pyrene derivative BP‐3T3. Both types of cells stopped their proliferation after 72 hr of treatment with the inhibitor. When DFMO was removed and cells were cultivated afterwards in fresh medium without the drug, untransformed cells resumed growth after a lag period, whereas transformed cells were unable to proliferate unless exogenous polyamine was added. These alterations showed a strict correlation with intracellular polyamine pools, since after removal of DFMO from the culture medium, polyamine concentrations increased to almost normal values in 3T3 cells, but remained at low levels or decreased even more in the transformed cells BP‐3T3. The analysis by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis of labeled proteins of both cell extracts has indicated that the described control of cell proliferation by intracellular polyamine levels might be related to the synthesis of at least two proteins with molecular weights of about 36,000 and 55,000 daltons. Copyright © 1983 Wiley‐Liss, Inc. |
| format |
JOUR |
| author |
Medrano, E.E. Goldemberg, S.H. Algranati, I.D. |
| author_facet |
Medrano, E.E. Goldemberg, S.H. Algranati, I.D. |
| author_sort |
Medrano, E.E. |
| title |
Differential effect of αdifluoromethyl‐ornithine on the proliferation of balb 3T3 and chemically transformed 3T3 cells |
| title_short |
Differential effect of αdifluoromethyl‐ornithine on the proliferation of balb 3T3 and chemically transformed 3T3 cells |
| title_full |
Differential effect of αdifluoromethyl‐ornithine on the proliferation of balb 3T3 and chemically transformed 3T3 cells |
| title_fullStr |
Differential effect of αdifluoromethyl‐ornithine on the proliferation of balb 3T3 and chemically transformed 3T3 cells |
| title_full_unstemmed |
Differential effect of αdifluoromethyl‐ornithine on the proliferation of balb 3T3 and chemically transformed 3T3 cells |
| title_sort |
differential effect of αdifluoromethyl‐ornithine on the proliferation of balb 3t3 and chemically transformed 3t3 cells |
| url |
http://hdl.handle.net/20.500.12110/paper_00219541_v117_n2_p141_Medrano |
| work_keys_str_mv |
AT medranoee differentialeffectofadifluoromethylornithineontheproliferationofbalb3t3andchemicallytransformed3t3cells AT goldembergsh differentialeffectofadifluoromethylornithineontheproliferationofbalb3t3andchemicallytransformed3t3cells AT algranatiid differentialeffectofadifluoromethylornithineontheproliferationofbalb3t3andchemicallytransformed3t3cells |
| _version_ |
1807323699209043968 |