A novel galacturonide from Xanthomonas campestris

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Enzyme preparations from Xanthomonas campestris incubated in the presence of UDP-[14C]GlcA and Mg2+ produced a lipophilic galacturonide with unusual properties. It was easily degraded by both mild acid treatment (0.01 M-HCl, 100°C, 10 min) and mild alkali treatment (0.06 M-NaOH, room temperature, 5...

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Detalles Bibliográficos
Autores principales: Baldessari, A., Ielpi, L., Dankert, M.A.
Formato: JOUR
Materias:
polysaccharide
radioisotope
article
nonhuman
priority journal
Xanthomonas
Xanthomonas campestris
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00221287_v136_n8_p1501_Baldessari
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00221287_v136_n8_p1501_Baldessari
record_format dspace
spelling todo:paper_00221287_v136_n8_p1501_Baldessari2023-10-03T14:27:21Z A novel galacturonide from Xanthomonas campestris Baldessari, A. Ielpi, L. Dankert, M.A. polysaccharide radioisotope article nonhuman priority journal Xanthomonas Xanthomonas Xanthomonas campestris Enzyme preparations from Xanthomonas campestris incubated in the presence of UDP-[14C]GlcA and Mg2+ produced a lipophilic galacturonide with unusual properties. It was easily degraded by both mild acid treatment (0.01 M-HCl, 100°C, 10 min) and mild alkali treatment (0.06 M-NaOH, room temperature, 5 min) releasing free [14C]galacturonic acid. The galacturonide appeared to be a single compound with one negative charge, as judged by TLC, paper electrophoresis and chromotography, LH-20 gel filtration and DEAE-cellulose column chromatography. Competition experiments indicated that the true glycosyl donor was UDP-GalA, in agreement with the detection of UDP-GlcA-4-epimerase activity in the crude enzyme preparation. The transglycosidase activity was located mainly in the membrane fraction. UDP inhibited the reaction and even produced some loss of label, suggesting an easily reversible reaction. UMP had almost no effect. Fil:Baldessari, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ielpi, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Dankert, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00221287_v136_n8_p1501_Baldessari
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic polysaccharide
radioisotope
article
nonhuman
priority journal
Xanthomonas
Xanthomonas
Xanthomonas campestris
spellingShingle polysaccharide
radioisotope
article
nonhuman
priority journal
Xanthomonas
Xanthomonas
Xanthomonas campestris
Baldessari, A.
Ielpi, L.
Dankert, M.A.
A novel galacturonide from Xanthomonas campestris
topic_facet polysaccharide
radioisotope
article
nonhuman
priority journal
Xanthomonas
Xanthomonas
Xanthomonas campestris
description Enzyme preparations from Xanthomonas campestris incubated in the presence of UDP-[14C]GlcA and Mg2+ produced a lipophilic galacturonide with unusual properties. It was easily degraded by both mild acid treatment (0.01 M-HCl, 100°C, 10 min) and mild alkali treatment (0.06 M-NaOH, room temperature, 5 min) releasing free [14C]galacturonic acid. The galacturonide appeared to be a single compound with one negative charge, as judged by TLC, paper electrophoresis and chromotography, LH-20 gel filtration and DEAE-cellulose column chromatography. Competition experiments indicated that the true glycosyl donor was UDP-GalA, in agreement with the detection of UDP-GlcA-4-epimerase activity in the crude enzyme preparation. The transglycosidase activity was located mainly in the membrane fraction. UDP inhibited the reaction and even produced some loss of label, suggesting an easily reversible reaction. UMP had almost no effect.
format JOUR
author Baldessari, A.
Ielpi, L.
Dankert, M.A.
author_facet Baldessari, A.
Ielpi, L.
Dankert, M.A.
author_sort Baldessari, A.
title A novel galacturonide from Xanthomonas campestris
title_short A novel galacturonide from Xanthomonas campestris
title_full A novel galacturonide from Xanthomonas campestris
title_fullStr A novel galacturonide from Xanthomonas campestris
title_full_unstemmed A novel galacturonide from Xanthomonas campestris
title_sort novel galacturonide from xanthomonas campestris
url http://hdl.handle.net/20.500.12110/paper_00221287_v136_n8_p1501_Baldessari
work_keys_str_mv AT baldessaria anovelgalacturonidefromxanthomonascampestris
AT ielpil anovelgalacturonidefromxanthomonascampestris
AT dankertma anovelgalacturonidefromxanthomonascampestris
AT baldessaria novelgalacturonidefromxanthomonascampestris
AT ielpil novelgalacturonidefromxanthomonascampestris
AT dankertma novelgalacturonidefromxanthomonascampestris
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