Ruv and recG genes and the induced precise excision of Tn10 in Escherichia coli
Induction of precise excision of Tn10 by UV or mitomycin C (MMC) is dependent on the expression of the SOS system. Ruv mutants of Escherichia coli, which are defective in DNA repair and recombination, showed diminished frequencies of both spontaneous and UV- or MMC-induced excision of Tn10 inserted...
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todo:paper_00275107_v311_n1_p103_Nagel2023-10-03T14:37:31Z Ruv and recG genes and the induced precise excision of Tn10 in Escherichia coli Nagel, R. Chan, A. Rosen, E. MMC induction RecGamutants Ruv mutants SOS genes Tn10 precise excision UV induction mitomycin c article controlled study dna excision escherichia coli gene mutant nonhuman priority journal transposon ultraviolet radiation Bacterial Proteins DNA Transposable Elements Escherichia coli Mitomycin Mutagens Mutation Support, Non-U.S. Gov't Ultraviolet Rays Escherichia coli Induction of precise excision of Tn10 by UV or mitomycin C (MMC) is dependent on the expression of the SOS system. Ruv mutants of Escherichia coli, which are defective in DNA repair and recombination, showed diminished frequencies of both spontaneous and UV- or MMC-induced excision of Tn10 inserted in gal. RecG mutants, which are also defective in DNA repair and recombination, showed decreased induction of Tn10 excision with MMC, but not after UV treatment. A recG ruv double mutant showed a greater decrease in induction of excision with MMC than either single mutant. One can speculate that the Ruv proteins, which are known to be involved in the resolution of Holliday junctions, might also be involved in the resolution of putative intermediates generated during the precise excision of Tn10. RecG protein, whose function partially overlaps those of Ruv proteins, might also have some role in this process. © 1994. Fil:Nagel, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rosen, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00275107_v311_n1_p103_Nagel |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
MMC induction RecGamutants Ruv mutants SOS genes Tn10 precise excision UV induction mitomycin c article controlled study dna excision escherichia coli gene mutant nonhuman priority journal transposon ultraviolet radiation Bacterial Proteins DNA Transposable Elements Escherichia coli Mitomycin Mutagens Mutation Support, Non-U.S. Gov't Ultraviolet Rays Escherichia coli |
| spellingShingle |
MMC induction RecGamutants Ruv mutants SOS genes Tn10 precise excision UV induction mitomycin c article controlled study dna excision escherichia coli gene mutant nonhuman priority journal transposon ultraviolet radiation Bacterial Proteins DNA Transposable Elements Escherichia coli Mitomycin Mutagens Mutation Support, Non-U.S. Gov't Ultraviolet Rays Escherichia coli Nagel, R. Chan, A. Rosen, E. Ruv and recG genes and the induced precise excision of Tn10 in Escherichia coli |
| topic_facet |
MMC induction RecGamutants Ruv mutants SOS genes Tn10 precise excision UV induction mitomycin c article controlled study dna excision escherichia coli gene mutant nonhuman priority journal transposon ultraviolet radiation Bacterial Proteins DNA Transposable Elements Escherichia coli Mitomycin Mutagens Mutation Support, Non-U.S. Gov't Ultraviolet Rays Escherichia coli |
| description |
Induction of precise excision of Tn10 by UV or mitomycin C (MMC) is dependent on the expression of the SOS system. Ruv mutants of Escherichia coli, which are defective in DNA repair and recombination, showed diminished frequencies of both spontaneous and UV- or MMC-induced excision of Tn10 inserted in gal. RecG mutants, which are also defective in DNA repair and recombination, showed decreased induction of Tn10 excision with MMC, but not after UV treatment. A recG ruv double mutant showed a greater decrease in induction of excision with MMC than either single mutant. One can speculate that the Ruv proteins, which are known to be involved in the resolution of Holliday junctions, might also be involved in the resolution of putative intermediates generated during the precise excision of Tn10. RecG protein, whose function partially overlaps those of Ruv proteins, might also have some role in this process. © 1994. |
| format |
JOUR |
| author |
Nagel, R. Chan, A. Rosen, E. |
| author_facet |
Nagel, R. Chan, A. Rosen, E. |
| author_sort |
Nagel, R. |
| title |
Ruv and recG genes and the induced precise excision of Tn10 in Escherichia coli |
| title_short |
Ruv and recG genes and the induced precise excision of Tn10 in Escherichia coli |
| title_full |
Ruv and recG genes and the induced precise excision of Tn10 in Escherichia coli |
| title_fullStr |
Ruv and recG genes and the induced precise excision of Tn10 in Escherichia coli |
| title_full_unstemmed |
Ruv and recG genes and the induced precise excision of Tn10 in Escherichia coli |
| title_sort |
ruv and recg genes and the induced precise excision of tn10 in escherichia coli |
| url |
http://hdl.handle.net/20.500.12110/paper_00275107_v311_n1_p103_Nagel |
| work_keys_str_mv |
AT nagelr ruvandrecggenesandtheinducedpreciseexcisionoftn10inescherichiacoli AT chana ruvandrecggenesandtheinducedpreciseexcisionoftn10inescherichiacoli AT rosene ruvandrecggenesandtheinducedpreciseexcisionoftn10inescherichiacoli |
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