Controls on nitrification in a water-limited ecosystem: Experimental inhibition of ammonia-oxidising bacteria in the Patagonian steppe
We studied controls on nitrification in an undisturbed water-limited ecosystem by inhibiting autotrophic nitrifying bacteria in soils with varying levels of vegetative cover. The activity of nitrifying bacteria was disrupted using nitrapyrin, 2-chloro-6-(trichloromethyl)-pyridine, under field condit...
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todo:paper_00380717_v35_n12_p1609_Lopez2023-10-03T14:48:20Z Controls on nitrification in a water-limited ecosystem: Experimental inhibition of ammonia-oxidising bacteria in the Patagonian steppe López, N.I. Austin, A.T. Sala, O.E. Méndez, B.S. Ammonia monooxygenase gene (amoA) Ammonia-oxidising bacteria Nitrapyrin Nitrification Nitrogen Semi-arid ecosystems Bacteria DNA Ecosystems Nitrification Nitrogen compounds Microenvironment Soil pollution ammonia nitrification nitrifying bacterium semiarid region steppe Argentina Patagonia Bacteria (microorganisms) Poaceae We studied controls on nitrification in an undisturbed water-limited ecosystem by inhibiting autotrophic nitrifying bacteria in soils with varying levels of vegetative cover. The activity of nitrifying bacteria was disrupted using nitrapyrin, 2-chloro-6-(trichloromethyl)-pyridine, under field conditions in three microenvironments (underneath shrubs, next to grasses and in bare soil). Ammonia-oxidising bacteria were detected by PCR analysis of DNA in soils. The inhibition of nitrification changed the concentrations of NO 3 - and NH4 + in the soil, while the microenvironment was most important in determining the response of bacteria to the inhibitor. Nitrapyrin application resulted in a significant (p < 0.05) reduction in soil NO3 - concentration (39%) and a significant increase (p < 0.001) in soil NH4 + concentration (41%). Untreated bare-soil microenvironments had the lowest concentrations of NH4 + (1.57 μg/g of dry soil) and NO3 - (0.49 μg/g of dry soil) when compared to the other microenvironments, and showed the highest impacts of nitrification inhibition. For example, NH4 + concentrations increased 288% and NO3 - concentrations decreased 60% in inhibited bare-soil microenvironments. In contrast, untreated microenvironments underneath shrubs had the highest levels of NH4 + (10.01 μg/g of dry soil) and NO3 - (0.69 μg/g of dry soil), but showed no significant effects of inhibition of nitrification on soil nitrogen concentrations. © 2003 Elsevier Ltd. All rights reserved. Fil:Méndez, B.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00380717_v35_n12_p1609_Lopez |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Ammonia monooxygenase gene (amoA) Ammonia-oxidising bacteria Nitrapyrin Nitrification Nitrogen Semi-arid ecosystems Bacteria DNA Ecosystems Nitrification Nitrogen compounds Microenvironment Soil pollution ammonia nitrification nitrifying bacterium semiarid region steppe Argentina Patagonia Bacteria (microorganisms) Poaceae |
| spellingShingle |
Ammonia monooxygenase gene (amoA) Ammonia-oxidising bacteria Nitrapyrin Nitrification Nitrogen Semi-arid ecosystems Bacteria DNA Ecosystems Nitrification Nitrogen compounds Microenvironment Soil pollution ammonia nitrification nitrifying bacterium semiarid region steppe Argentina Patagonia Bacteria (microorganisms) Poaceae López, N.I. Austin, A.T. Sala, O.E. Méndez, B.S. Controls on nitrification in a water-limited ecosystem: Experimental inhibition of ammonia-oxidising bacteria in the Patagonian steppe |
| topic_facet |
Ammonia monooxygenase gene (amoA) Ammonia-oxidising bacteria Nitrapyrin Nitrification Nitrogen Semi-arid ecosystems Bacteria DNA Ecosystems Nitrification Nitrogen compounds Microenvironment Soil pollution ammonia nitrification nitrifying bacterium semiarid region steppe Argentina Patagonia Bacteria (microorganisms) Poaceae |
| description |
We studied controls on nitrification in an undisturbed water-limited ecosystem by inhibiting autotrophic nitrifying bacteria in soils with varying levels of vegetative cover. The activity of nitrifying bacteria was disrupted using nitrapyrin, 2-chloro-6-(trichloromethyl)-pyridine, under field conditions in three microenvironments (underneath shrubs, next to grasses and in bare soil). Ammonia-oxidising bacteria were detected by PCR analysis of DNA in soils. The inhibition of nitrification changed the concentrations of NO 3 - and NH4 + in the soil, while the microenvironment was most important in determining the response of bacteria to the inhibitor. Nitrapyrin application resulted in a significant (p < 0.05) reduction in soil NO3 - concentration (39%) and a significant increase (p < 0.001) in soil NH4 + concentration (41%). Untreated bare-soil microenvironments had the lowest concentrations of NH4 + (1.57 μg/g of dry soil) and NO3 - (0.49 μg/g of dry soil) when compared to the other microenvironments, and showed the highest impacts of nitrification inhibition. For example, NH4 + concentrations increased 288% and NO3 - concentrations decreased 60% in inhibited bare-soil microenvironments. In contrast, untreated microenvironments underneath shrubs had the highest levels of NH4 + (10.01 μg/g of dry soil) and NO3 - (0.69 μg/g of dry soil), but showed no significant effects of inhibition of nitrification on soil nitrogen concentrations. © 2003 Elsevier Ltd. All rights reserved. |
| format |
JOUR |
| author |
López, N.I. Austin, A.T. Sala, O.E. Méndez, B.S. |
| author_facet |
López, N.I. Austin, A.T. Sala, O.E. Méndez, B.S. |
| author_sort |
López, N.I. |
| title |
Controls on nitrification in a water-limited ecosystem: Experimental inhibition of ammonia-oxidising bacteria in the Patagonian steppe |
| title_short |
Controls on nitrification in a water-limited ecosystem: Experimental inhibition of ammonia-oxidising bacteria in the Patagonian steppe |
| title_full |
Controls on nitrification in a water-limited ecosystem: Experimental inhibition of ammonia-oxidising bacteria in the Patagonian steppe |
| title_fullStr |
Controls on nitrification in a water-limited ecosystem: Experimental inhibition of ammonia-oxidising bacteria in the Patagonian steppe |
| title_full_unstemmed |
Controls on nitrification in a water-limited ecosystem: Experimental inhibition of ammonia-oxidising bacteria in the Patagonian steppe |
| title_sort |
controls on nitrification in a water-limited ecosystem: experimental inhibition of ammonia-oxidising bacteria in the patagonian steppe |
| url |
http://hdl.handle.net/20.500.12110/paper_00380717_v35_n12_p1609_Lopez |
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