Endothelin-1 -induced incorporation of cholesterol into rat adrenals
The effect of endothelin-1 (ET-1) on cholesterol uptake by adrenal cortex was evaluated through several experimental approaches: infusion of ET-1 followed by measurement of endogenous cholesterol in excised adrenals; infusion of ET-1 followed by tritiated cholesterol incorporation into adrenal quart...
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todo:paper_0039128X_v61_n5_p317_Romero2023-10-03T14:49:23Z Endothelin-1 -induced incorporation of cholesterol into rat adrenals Romero, D.G. Pecci, A. Lantos, C.P. Cozza, E.N. Adrenal Cholesterol Endothelin Steroidogenesis 3(or 17)beta hydroxysteroid dehydrogenase aminoglutethimide cholesterol cholesterol monooxygenase (side chain cleaving) endothelin 1 endothelin receptor isomerase tritium adrenal cell adrenal cortex animal cell animal experiment animal tissue article cell culture cell fractionation cholesterol metabolism cholesterol transport controlled study enzyme activity infusion isotope labeling male nonhuman radioactivity rat Adrenal Glands Aldosterone Aminoglutethimide Animals Cholesterol Endothelin-1 Infusions, Intravenous Male Rats Rats, Inbred Strains Animalia Tritium The effect of endothelin-1 (ET-1) on cholesterol uptake by adrenal cortex was evaluated through several experimental approaches: infusion of ET-1 followed by measurement of endogenous cholesterol in excised adrenals; infusion of ET-1 followed by tritiated cholesterol incorporation into adrenal quarters in vitro; coinfusion of ET-1 with tritiated cholesterol-enriched serum and determination of adrenal-associated radioactivity; and tritiated cholesterol incorporation in incubations of adrenal cells. In all cases ET-1 increased cholesterol uptake. Subcellular fractionation showed an ET-1-mediated augmentation in mitochondrial fraction. This increase was mediated by the subpopulation B of adrenal receptors for ET-1. In addition, ET-1 also increased cytochrome P450-SCC (side-chain cleavage) activity. © 1996 by Elsevier Science Inc. Fil:Romero, D.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pecci, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cozza, E.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0039128X_v61_n5_p317_Romero |
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Universidad de Buenos Aires |
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I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Adrenal Cholesterol Endothelin Steroidogenesis 3(or 17)beta hydroxysteroid dehydrogenase aminoglutethimide cholesterol cholesterol monooxygenase (side chain cleaving) endothelin 1 endothelin receptor isomerase tritium adrenal cell adrenal cortex animal cell animal experiment animal tissue article cell culture cell fractionation cholesterol metabolism cholesterol transport controlled study enzyme activity infusion isotope labeling male nonhuman radioactivity rat Adrenal Glands Aldosterone Aminoglutethimide Animals Cholesterol Endothelin-1 Infusions, Intravenous Male Rats Rats, Inbred Strains Animalia Tritium |
| spellingShingle |
Adrenal Cholesterol Endothelin Steroidogenesis 3(or 17)beta hydroxysteroid dehydrogenase aminoglutethimide cholesterol cholesterol monooxygenase (side chain cleaving) endothelin 1 endothelin receptor isomerase tritium adrenal cell adrenal cortex animal cell animal experiment animal tissue article cell culture cell fractionation cholesterol metabolism cholesterol transport controlled study enzyme activity infusion isotope labeling male nonhuman radioactivity rat Adrenal Glands Aldosterone Aminoglutethimide Animals Cholesterol Endothelin-1 Infusions, Intravenous Male Rats Rats, Inbred Strains Animalia Tritium Romero, D.G. Pecci, A. Lantos, C.P. Cozza, E.N. Endothelin-1 -induced incorporation of cholesterol into rat adrenals |
| topic_facet |
Adrenal Cholesterol Endothelin Steroidogenesis 3(or 17)beta hydroxysteroid dehydrogenase aminoglutethimide cholesterol cholesterol monooxygenase (side chain cleaving) endothelin 1 endothelin receptor isomerase tritium adrenal cell adrenal cortex animal cell animal experiment animal tissue article cell culture cell fractionation cholesterol metabolism cholesterol transport controlled study enzyme activity infusion isotope labeling male nonhuman radioactivity rat Adrenal Glands Aldosterone Aminoglutethimide Animals Cholesterol Endothelin-1 Infusions, Intravenous Male Rats Rats, Inbred Strains Animalia Tritium |
| description |
The effect of endothelin-1 (ET-1) on cholesterol uptake by adrenal cortex was evaluated through several experimental approaches: infusion of ET-1 followed by measurement of endogenous cholesterol in excised adrenals; infusion of ET-1 followed by tritiated cholesterol incorporation into adrenal quarters in vitro; coinfusion of ET-1 with tritiated cholesterol-enriched serum and determination of adrenal-associated radioactivity; and tritiated cholesterol incorporation in incubations of adrenal cells. In all cases ET-1 increased cholesterol uptake. Subcellular fractionation showed an ET-1-mediated augmentation in mitochondrial fraction. This increase was mediated by the subpopulation B of adrenal receptors for ET-1. In addition, ET-1 also increased cytochrome P450-SCC (side-chain cleavage) activity. © 1996 by Elsevier Science Inc. |
| format |
JOUR |
| author |
Romero, D.G. Pecci, A. Lantos, C.P. Cozza, E.N. |
| author_facet |
Romero, D.G. Pecci, A. Lantos, C.P. Cozza, E.N. |
| author_sort |
Romero, D.G. |
| title |
Endothelin-1 -induced incorporation of cholesterol into rat adrenals |
| title_short |
Endothelin-1 -induced incorporation of cholesterol into rat adrenals |
| title_full |
Endothelin-1 -induced incorporation of cholesterol into rat adrenals |
| title_fullStr |
Endothelin-1 -induced incorporation of cholesterol into rat adrenals |
| title_full_unstemmed |
Endothelin-1 -induced incorporation of cholesterol into rat adrenals |
| title_sort |
endothelin-1 -induced incorporation of cholesterol into rat adrenals |
| url |
http://hdl.handle.net/20.500.12110/paper_0039128X_v61_n5_p317_Romero |
| work_keys_str_mv |
AT romerodg endothelin1inducedincorporationofcholesterolintoratadrenals AT peccia endothelin1inducedincorporationofcholesterolintoratadrenals AT lantoscp endothelin1inducedincorporationofcholesterolintoratadrenals AT cozzaen endothelin1inducedincorporationofcholesterolintoratadrenals |
| _version_ |
1807323050566221824 |