A simplified approach to the determination of N-nitroso glyphosate in technical glyphosate using HPLC with post-derivatization and colorimetric detection

A simple and sensitive HPLC post-derivatization method with colorimetric detection has been developed for the determination of N-nitroso glyphosate in samples of technical glyphosate. Separation of the analyte was accomplished using an anionic exchange resin (2.50 mm × 4.00 mm i.d., 15 μm particle s...

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Autores principales: Kim, M., Stripeikis, J., Iñón, F., Tudino, M.
Formato: JOUR
Materias:
Glyphosate
HPLC with post-derivatization
N-nitrosoglyphosate
Colorimetry
Energy utilization
High performance liquid chromatography
Ion exchange
Particle size
Post-derivatization
Nitrogen compounds
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00399140_v72_n3_p1054_Kim
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling todo:paper_00399140_v72_n3_p1054_Kim2023-10-03T14:50:01Z A simplified approach to the determination of N-nitroso glyphosate in technical glyphosate using HPLC with post-derivatization and colorimetric detection Kim, M. Stripeikis, J. Iñón, F. Tudino, M. Glyphosate HPLC with post-derivatization N-nitrosoglyphosate Colorimetry Energy utilization High performance liquid chromatography Ion exchange Particle size Glyphosate N-nitrosoglyphosate Post-derivatization Nitrogen compounds A simple and sensitive HPLC post-derivatization method with colorimetric detection has been developed for the determination of N-nitroso glyphosate in samples of technical glyphosate. Separation of the analyte was accomplished using an anionic exchange resin (2.50 mm × 4.00 mm i.d., 15 μm particle size, functional group: quaternary ammonium salt) with Na2SO4 0.0075 M (pH 11.5) (flow rate: 1.0 mL min-1) as mobile phase. After separation, the eluate was derivatized with a colorimetric reagent containing sulfanilamide 0.3% (w/v), [N-(1-naphtil)ethilendiamine] 0.03% (w/v) and HCl 4.5 M in a thermostatized bath at 95 °C. Detection was performed at 546 nm. All stages of the analytical procedure were optimized taking into account the concept of analytical minimalism: less operation times and costs; lower sample, reagents and energy consumption and minimal waste. The limit of detection (k = 3) calculated for 10 blank replicates was 0.04 mg L-1 (0.8 mg kg-1) in the solid sample which is lower than the maximum tolerable accepted by the Food and Agriculture Organization of the United Nations. © 2007 Elsevier B.V. All rights reserved. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00399140_v72_n3_p1054_Kim
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Glyphosate
HPLC with post-derivatization
N-nitrosoglyphosate
Colorimetry
Energy utilization
High performance liquid chromatography
Ion exchange
Particle size
Glyphosate
N-nitrosoglyphosate
Post-derivatization
Nitrogen compounds
spellingShingle Glyphosate
HPLC with post-derivatization
N-nitrosoglyphosate
Colorimetry
Energy utilization
High performance liquid chromatography
Ion exchange
Particle size
Glyphosate
N-nitrosoglyphosate
Post-derivatization
Nitrogen compounds
Kim, M.
Stripeikis, J.
Iñón, F.
Tudino, M.
A simplified approach to the determination of N-nitroso glyphosate in technical glyphosate using HPLC with post-derivatization and colorimetric detection
topic_facet Glyphosate
HPLC with post-derivatization
N-nitrosoglyphosate
Colorimetry
Energy utilization
High performance liquid chromatography
Ion exchange
Particle size
Glyphosate
N-nitrosoglyphosate
Post-derivatization
Nitrogen compounds
description A simple and sensitive HPLC post-derivatization method with colorimetric detection has been developed for the determination of N-nitroso glyphosate in samples of technical glyphosate. Separation of the analyte was accomplished using an anionic exchange resin (2.50 mm × 4.00 mm i.d., 15 μm particle size, functional group: quaternary ammonium salt) with Na2SO4 0.0075 M (pH 11.5) (flow rate: 1.0 mL min-1) as mobile phase. After separation, the eluate was derivatized with a colorimetric reagent containing sulfanilamide 0.3% (w/v), [N-(1-naphtil)ethilendiamine] 0.03% (w/v) and HCl 4.5 M in a thermostatized bath at 95 °C. Detection was performed at 546 nm. All stages of the analytical procedure were optimized taking into account the concept of analytical minimalism: less operation times and costs; lower sample, reagents and energy consumption and minimal waste. The limit of detection (k = 3) calculated for 10 blank replicates was 0.04 mg L-1 (0.8 mg kg-1) in the solid sample which is lower than the maximum tolerable accepted by the Food and Agriculture Organization of the United Nations. © 2007 Elsevier B.V. All rights reserved.
format JOUR
author Kim, M.
Stripeikis, J.
Iñón, F.
Tudino, M.
author_facet Kim, M.
Stripeikis, J.
Iñón, F.
Tudino, M.
author_sort Kim, M.
title A simplified approach to the determination of N-nitroso glyphosate in technical glyphosate using HPLC with post-derivatization and colorimetric detection
title_short A simplified approach to the determination of N-nitroso glyphosate in technical glyphosate using HPLC with post-derivatization and colorimetric detection
title_full A simplified approach to the determination of N-nitroso glyphosate in technical glyphosate using HPLC with post-derivatization and colorimetric detection
title_fullStr A simplified approach to the determination of N-nitroso glyphosate in technical glyphosate using HPLC with post-derivatization and colorimetric detection
title_full_unstemmed A simplified approach to the determination of N-nitroso glyphosate in technical glyphosate using HPLC with post-derivatization and colorimetric detection
title_sort simplified approach to the determination of n-nitroso glyphosate in technical glyphosate using hplc with post-derivatization and colorimetric detection
url http://hdl.handle.net/20.500.12110/paper_00399140_v72_n3_p1054_Kim
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