Daily treatment with α-naphthoflavone enhances follicular growth and ovulation rate in the rat
The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor and the first protein involved in a variety of physiological and toxicological processes, including those of xenobiotic metabolizing enzymes. AhR has been found in the ovary of many species and seems to mediate the ovaria...
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todo:paper_0041008X_v252_n1_p11_Barreiro2023-10-03T14:51:15Z Daily treatment with α-naphthoflavone enhances follicular growth and ovulation rate in the rat Barreiro, K.A. Di Yorio, M.P. Artillo-Guida, R.D. Paz, D.A. Faletti, A.G. α-naphthoflavone AhR Follicular growth Ovulation alpha naphthoflavone chorionic gonadotropin animal experiment animal tissue article corpus luteum drug mechanism female immunohistochemistry nonhuman oocyte organ weight ovary ovary follicle development oviduct ovulation rat Animals Benzoflavones Dose-Response Relationship, Drug Drug Administration Schedule Female Humans Ovarian Follicle Ovulation Random Allocation Rats Rats, Sprague-Dawley Receptors, Aryl Hydrocarbon Rattus The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor and the first protein involved in a variety of physiological and toxicological processes, including those of xenobiotic metabolizing enzymes. AhR has been found in the ovary of many species and seems to mediate the ovarian toxicity of many environmental contaminants, which are AhR ligands. However, the role of AhR in the ovarian function is unknown. Therefore, the aim of this work was to study the action of α-naphthoflavone (αNF), known to be an AhR antagonist, on both follicular growth and ovulation. Immature Sprague-Dawley rats were daily injected intraperitoneally with αNF (0.1-80. mg/kg) or vehicle for 12. days, and primed with gonadotrophins (eCG/hCG) to induce follicular growth and ovulation. Ovaries were obtained 20. h after hCG administration. By means of immunohistochemistry, we found that the numbers of primordial, primary and antral follicles were increased in rats treated with 80. mg/kg αNF and that there were no differences with other doses. Likewise, the ovarian weight and the ovulation rate, measured by both number of oocytes within oviducts and corpora lutea in ovarian sections, were increased when the rats received either 1 or 10. mg/kg daily. Although further studies are necessary to know the mechanism of action of αNF, it is possible that the different ovarian processes can be differentially responsive to the presence of different levels of αNF, and that the same or different endogenous AhR ligands can be involved in these ovarian processes in a cell type-dependent manner. © 2011 Elsevier Inc. Fil:Barreiro, K.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Di Yorio, M.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Paz, D.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Faletti, A.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0041008X_v252_n1_p11_Barreiro |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
α-naphthoflavone AhR Follicular growth Ovulation alpha naphthoflavone chorionic gonadotropin animal experiment animal tissue article corpus luteum drug mechanism female immunohistochemistry nonhuman oocyte organ weight ovary ovary follicle development oviduct ovulation rat Animals Benzoflavones Dose-Response Relationship, Drug Drug Administration Schedule Female Humans Ovarian Follicle Ovulation Random Allocation Rats Rats, Sprague-Dawley Receptors, Aryl Hydrocarbon Rattus |
| spellingShingle |
α-naphthoflavone AhR Follicular growth Ovulation alpha naphthoflavone chorionic gonadotropin animal experiment animal tissue article corpus luteum drug mechanism female immunohistochemistry nonhuman oocyte organ weight ovary ovary follicle development oviduct ovulation rat Animals Benzoflavones Dose-Response Relationship, Drug Drug Administration Schedule Female Humans Ovarian Follicle Ovulation Random Allocation Rats Rats, Sprague-Dawley Receptors, Aryl Hydrocarbon Rattus Barreiro, K.A. Di Yorio, M.P. Artillo-Guida, R.D. Paz, D.A. Faletti, A.G. Daily treatment with α-naphthoflavone enhances follicular growth and ovulation rate in the rat |
| topic_facet |
α-naphthoflavone AhR Follicular growth Ovulation alpha naphthoflavone chorionic gonadotropin animal experiment animal tissue article corpus luteum drug mechanism female immunohistochemistry nonhuman oocyte organ weight ovary ovary follicle development oviduct ovulation rat Animals Benzoflavones Dose-Response Relationship, Drug Drug Administration Schedule Female Humans Ovarian Follicle Ovulation Random Allocation Rats Rats, Sprague-Dawley Receptors, Aryl Hydrocarbon Rattus |
| description |
The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor and the first protein involved in a variety of physiological and toxicological processes, including those of xenobiotic metabolizing enzymes. AhR has been found in the ovary of many species and seems to mediate the ovarian toxicity of many environmental contaminants, which are AhR ligands. However, the role of AhR in the ovarian function is unknown. Therefore, the aim of this work was to study the action of α-naphthoflavone (αNF), known to be an AhR antagonist, on both follicular growth and ovulation. Immature Sprague-Dawley rats were daily injected intraperitoneally with αNF (0.1-80. mg/kg) or vehicle for 12. days, and primed with gonadotrophins (eCG/hCG) to induce follicular growth and ovulation. Ovaries were obtained 20. h after hCG administration. By means of immunohistochemistry, we found that the numbers of primordial, primary and antral follicles were increased in rats treated with 80. mg/kg αNF and that there were no differences with other doses. Likewise, the ovarian weight and the ovulation rate, measured by both number of oocytes within oviducts and corpora lutea in ovarian sections, were increased when the rats received either 1 or 10. mg/kg daily. Although further studies are necessary to know the mechanism of action of αNF, it is possible that the different ovarian processes can be differentially responsive to the presence of different levels of αNF, and that the same or different endogenous AhR ligands can be involved in these ovarian processes in a cell type-dependent manner. © 2011 Elsevier Inc. |
| format |
JOUR |
| author |
Barreiro, K.A. Di Yorio, M.P. Artillo-Guida, R.D. Paz, D.A. Faletti, A.G. |
| author_facet |
Barreiro, K.A. Di Yorio, M.P. Artillo-Guida, R.D. Paz, D.A. Faletti, A.G. |
| author_sort |
Barreiro, K.A. |
| title |
Daily treatment with α-naphthoflavone enhances follicular growth and ovulation rate in the rat |
| title_short |
Daily treatment with α-naphthoflavone enhances follicular growth and ovulation rate in the rat |
| title_full |
Daily treatment with α-naphthoflavone enhances follicular growth and ovulation rate in the rat |
| title_fullStr |
Daily treatment with α-naphthoflavone enhances follicular growth and ovulation rate in the rat |
| title_full_unstemmed |
Daily treatment with α-naphthoflavone enhances follicular growth and ovulation rate in the rat |
| title_sort |
daily treatment with α-naphthoflavone enhances follicular growth and ovulation rate in the rat |
| url |
http://hdl.handle.net/20.500.12110/paper_0041008X_v252_n1_p11_Barreiro |
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