Hepatitis B virus antigens in peripheral blood mononuclear cells during the course of viral infection
We studied the expression of surface (HBsAg) and core (HBcAg) proteins of hepatitis B virus (HBV) on the surface of peripheral blood mononuclear cells (PBMC) from HBV-infected patients. A total of 122 patients with different liver viral diseases was analyzed by indirect immunofluorescence with monoc...
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todo:paper_00901229_v70_n2_p99_Roisman2023-10-03T14:54:32Z Hepatitis B virus antigens in peripheral blood mononuclear cells during the course of viral infection Roisman, F.R. Castello, A. Fainboim, H. Morelli, A. Fainboim, L. hepatitis b core antigen hepatitis b surface antigen virus antigen adolescent adult aged article controlled study female hepatitis b virus human human cell immunoelectron microscopy immunofluorescence major clinical study male mononuclear cell priority journal virus infection Acute Disease Adolescent Adult Aged Female Flow Cytometry Hepatitis Hepatitis B Hepatitis B Core Antigens Hepatitis B Surface Antigens Hepatitis, Chronic Human Leukocytes, Mononuclear Male Middle Age Support, Non-U.S. Gov't We studied the expression of surface (HBsAg) and core (HBcAg) proteins of hepatitis B virus (HBV) on the surface of peripheral blood mononuclear cells (PBMC) from HBV-infected patients. A total of 122 patients with different liver viral diseases was analyzed by indirect immunofluorescence with monoclonal antibodies. The 35 patients with HBV chronic active hepatitis (CAH) and 38 of 60 patients with acute hepatitis B (63%) expressed HBsAg on the PBMC. No expression was detected on the cells from both normal and HBV-unrelated viral hepatitis control groups. Serial follow-up of patients with acute hepatitis B showed that HBsAg expression by PBMC tended to be undetectable 4 months after the onset of the disease and at the same time the clinical improvement was evident. Cell cultures of EBV-transformed B lymphocytes were established from PBMC of HBV-infected patients; immunoelectron microscopy demonstrated the HBsAg on the cellular membrane. One-third of HBV-infected patients who were studied showed the expression of HBcAg by PBMC. HBcAg was detected in patients with acute hepatitis B at the early stage of infection. The cells of these patients also expressed HBsAg in PBMC. In CAH patients, a positive association was observed between the expression of HBcAg and the presence of serum HBeAg. © 1994 Academic Press. All rights reserved. Fil:Roisman, F.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00901229_v70_n2_p99_Roisman |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
hepatitis b core antigen hepatitis b surface antigen virus antigen adolescent adult aged article controlled study female hepatitis b virus human human cell immunoelectron microscopy immunofluorescence major clinical study male mononuclear cell priority journal virus infection Acute Disease Adolescent Adult Aged Female Flow Cytometry Hepatitis Hepatitis B Hepatitis B Core Antigens Hepatitis B Surface Antigens Hepatitis, Chronic Human Leukocytes, Mononuclear Male Middle Age Support, Non-U.S. Gov't |
| spellingShingle |
hepatitis b core antigen hepatitis b surface antigen virus antigen adolescent adult aged article controlled study female hepatitis b virus human human cell immunoelectron microscopy immunofluorescence major clinical study male mononuclear cell priority journal virus infection Acute Disease Adolescent Adult Aged Female Flow Cytometry Hepatitis Hepatitis B Hepatitis B Core Antigens Hepatitis B Surface Antigens Hepatitis, Chronic Human Leukocytes, Mononuclear Male Middle Age Support, Non-U.S. Gov't Roisman, F.R. Castello, A. Fainboim, H. Morelli, A. Fainboim, L. Hepatitis B virus antigens in peripheral blood mononuclear cells during the course of viral infection |
| topic_facet |
hepatitis b core antigen hepatitis b surface antigen virus antigen adolescent adult aged article controlled study female hepatitis b virus human human cell immunoelectron microscopy immunofluorescence major clinical study male mononuclear cell priority journal virus infection Acute Disease Adolescent Adult Aged Female Flow Cytometry Hepatitis Hepatitis B Hepatitis B Core Antigens Hepatitis B Surface Antigens Hepatitis, Chronic Human Leukocytes, Mononuclear Male Middle Age Support, Non-U.S. Gov't |
| description |
We studied the expression of surface (HBsAg) and core (HBcAg) proteins of hepatitis B virus (HBV) on the surface of peripheral blood mononuclear cells (PBMC) from HBV-infected patients. A total of 122 patients with different liver viral diseases was analyzed by indirect immunofluorescence with monoclonal antibodies. The 35 patients with HBV chronic active hepatitis (CAH) and 38 of 60 patients with acute hepatitis B (63%) expressed HBsAg on the PBMC. No expression was detected on the cells from both normal and HBV-unrelated viral hepatitis control groups. Serial follow-up of patients with acute hepatitis B showed that HBsAg expression by PBMC tended to be undetectable 4 months after the onset of the disease and at the same time the clinical improvement was evident. Cell cultures of EBV-transformed B lymphocytes were established from PBMC of HBV-infected patients; immunoelectron microscopy demonstrated the HBsAg on the cellular membrane. One-third of HBV-infected patients who were studied showed the expression of HBcAg by PBMC. HBcAg was detected in patients with acute hepatitis B at the early stage of infection. The cells of these patients also expressed HBsAg in PBMC. In CAH patients, a positive association was observed between the expression of HBcAg and the presence of serum HBeAg. © 1994 Academic Press. All rights reserved. |
| format |
JOUR |
| author |
Roisman, F.R. Castello, A. Fainboim, H. Morelli, A. Fainboim, L. |
| author_facet |
Roisman, F.R. Castello, A. Fainboim, H. Morelli, A. Fainboim, L. |
| author_sort |
Roisman, F.R. |
| title |
Hepatitis B virus antigens in peripheral blood mononuclear cells during the course of viral infection |
| title_short |
Hepatitis B virus antigens in peripheral blood mononuclear cells during the course of viral infection |
| title_full |
Hepatitis B virus antigens in peripheral blood mononuclear cells during the course of viral infection |
| title_fullStr |
Hepatitis B virus antigens in peripheral blood mononuclear cells during the course of viral infection |
| title_full_unstemmed |
Hepatitis B virus antigens in peripheral blood mononuclear cells during the course of viral infection |
| title_sort |
hepatitis b virus antigens in peripheral blood mononuclear cells during the course of viral infection |
| url |
http://hdl.handle.net/20.500.12110/paper_00901229_v70_n2_p99_Roisman |
| work_keys_str_mv |
AT roismanfr hepatitisbvirusantigensinperipheralbloodmononuclearcellsduringthecourseofviralinfection AT castelloa hepatitisbvirusantigensinperipheralbloodmononuclearcellsduringthecourseofviralinfection AT fainboimh hepatitisbvirusantigensinperipheralbloodmononuclearcellsduringthecourseofviralinfection AT morellia hepatitisbvirusantigensinperipheralbloodmononuclearcellsduringthecourseofviralinfection AT fainboiml hepatitisbvirusantigensinperipheralbloodmononuclearcellsduringthecourseofviralinfection |
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1807322220261801984 |