New mobilizable vectors suitable for gene replacement in gram-negative bacteria and their use in mapping of the 3' end of the Xanthomonas campestris pv. campestris gum operon

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We describe useful vectors to select double-crossover events directly in site-directed marker exchange mutagenesis in gram-negative bacteria. These vectors contain the gusA marker gene, providing colorimetric screens to identify bacteria harboring those sequences. The applicability of these vectors...

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Autores principales:	Katzen, F., Becker, A., Ielmini, M.V., Oddo, C.G., Ielpi, L.
Formato:	JOUR
Materias:	xanthan article bacterium identification biosynthesis cloning vector colorimetry crossing over gene disruption gene mapping gram negative bacterium nonhuman nucleotide sequence operon reverse transcription polymerase chain reaction sequence analysis site directed mutagenesis xanthomonas Base Sequence Chromosome Mapping DNA Primers DNA, Bacterial Genes, Bacterial Genetic Vectors Gram-Negative Bacteria Molecular Sequence Data Mutagenesis, Site-Directed Operon Reverse Transcriptase Polymerase Chain Reaction Xanthomonas campestris Bacteria (microorganisms) Negibacteria Xanthomonas Xanthomonas campestris pv. campestris
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00992240_v65_n1_p278_Katzen
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00992240_v65_n1_p278_Katzen
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spelling	todo:paper_00992240_v65_n1_p278_Katzen2023-10-03T14:57:04Z New mobilizable vectors suitable for gene replacement in gram-negative bacteria and their use in mapping of the 3' end of the Xanthomonas campestris pv. campestris gum operon Katzen, F. Becker, A. Ielmini, M.V. Oddo, C.G. Ielpi, L. xanthan article bacterium identification biosynthesis cloning vector colorimetry crossing over gene disruption gene mapping gram negative bacterium nonhuman nucleotide sequence operon reverse transcription polymerase chain reaction sequence analysis site directed mutagenesis xanthomonas Base Sequence Chromosome Mapping DNA Primers DNA, Bacterial Genes, Bacterial Genetic Vectors Gram-Negative Bacteria Molecular Sequence Data Mutagenesis, Site-Directed Operon Reverse Transcriptase Polymerase Chain Reaction Xanthomonas campestris Bacteria (microorganisms) Negibacteria Xanthomonas Xanthomonas campestris Xanthomonas campestris pv. campestris We describe useful vectors to select double-crossover events directly in site-directed marker exchange mutagenesis in gram-negative bacteria. These vectors contain the gusA marker gene, providing colorimetric screens to identify bacteria harboring those sequences. The applicability of these vectors was shown by mapping the 3' end of the Xanthomonas campestris gum operon, involved in biosynthesis of xanthan. Fil:Katzen, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ielmini, M.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ielpi, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00992240_v65_n1_p278_Katzen
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	xanthan article bacterium identification biosynthesis cloning vector colorimetry crossing over gene disruption gene mapping gram negative bacterium nonhuman nucleotide sequence operon reverse transcription polymerase chain reaction sequence analysis site directed mutagenesis xanthomonas Base Sequence Chromosome Mapping DNA Primers DNA, Bacterial Genes, Bacterial Genetic Vectors Gram-Negative Bacteria Molecular Sequence Data Mutagenesis, Site-Directed Operon Reverse Transcriptase Polymerase Chain Reaction Xanthomonas campestris Bacteria (microorganisms) Negibacteria Xanthomonas Xanthomonas campestris Xanthomonas campestris pv. campestris
spellingShingle	xanthan article bacterium identification biosynthesis cloning vector colorimetry crossing over gene disruption gene mapping gram negative bacterium nonhuman nucleotide sequence operon reverse transcription polymerase chain reaction sequence analysis site directed mutagenesis xanthomonas Base Sequence Chromosome Mapping DNA Primers DNA, Bacterial Genes, Bacterial Genetic Vectors Gram-Negative Bacteria Molecular Sequence Data Mutagenesis, Site-Directed Operon Reverse Transcriptase Polymerase Chain Reaction Xanthomonas campestris Bacteria (microorganisms) Negibacteria Xanthomonas Xanthomonas campestris Xanthomonas campestris pv. campestris Katzen, F. Becker, A. Ielmini, M.V. Oddo, C.G. Ielpi, L. New mobilizable vectors suitable for gene replacement in gram-negative bacteria and their use in mapping of the 3' end of the Xanthomonas campestris pv. campestris gum operon
topic_facet	xanthan article bacterium identification biosynthesis cloning vector colorimetry crossing over gene disruption gene mapping gram negative bacterium nonhuman nucleotide sequence operon reverse transcription polymerase chain reaction sequence analysis site directed mutagenesis xanthomonas Base Sequence Chromosome Mapping DNA Primers DNA, Bacterial Genes, Bacterial Genetic Vectors Gram-Negative Bacteria Molecular Sequence Data Mutagenesis, Site-Directed Operon Reverse Transcriptase Polymerase Chain Reaction Xanthomonas campestris Bacteria (microorganisms) Negibacteria Xanthomonas Xanthomonas campestris Xanthomonas campestris pv. campestris
description	We describe useful vectors to select double-crossover events directly in site-directed marker exchange mutagenesis in gram-negative bacteria. These vectors contain the gusA marker gene, providing colorimetric screens to identify bacteria harboring those sequences. The applicability of these vectors was shown by mapping the 3' end of the Xanthomonas campestris gum operon, involved in biosynthesis of xanthan.
format	JOUR
author	Katzen, F. Becker, A. Ielmini, M.V. Oddo, C.G. Ielpi, L.
author_facet	Katzen, F. Becker, A. Ielmini, M.V. Oddo, C.G. Ielpi, L.
author_sort	Katzen, F.
title	New mobilizable vectors suitable for gene replacement in gram-negative bacteria and their use in mapping of the 3' end of the Xanthomonas campestris pv. campestris gum operon
title_short	New mobilizable vectors suitable for gene replacement in gram-negative bacteria and their use in mapping of the 3' end of the Xanthomonas campestris pv. campestris gum operon
title_full	New mobilizable vectors suitable for gene replacement in gram-negative bacteria and their use in mapping of the 3' end of the Xanthomonas campestris pv. campestris gum operon
title_fullStr	New mobilizable vectors suitable for gene replacement in gram-negative bacteria and their use in mapping of the 3' end of the Xanthomonas campestris pv. campestris gum operon
title_full_unstemmed	New mobilizable vectors suitable for gene replacement in gram-negative bacteria and their use in mapping of the 3' end of the Xanthomonas campestris pv. campestris gum operon
title_sort	new mobilizable vectors suitable for gene replacement in gram-negative bacteria and their use in mapping of the 3' end of the xanthomonas campestris pv. campestris gum operon
url	http://hdl.handle.net/20.500.12110/paper_00992240_v65_n1_p278_Katzen
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New mobilizable vectors suitable for gene replacement in gram-negative bacteria and their use in mapping of the 3' end of the Xanthomonas campestris pv. campestris gum operon

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