Polyhydroxyalkanoate degradation is associated with nucleotide accumulation and enhances stress resistance and survival of Pseudomonas oleovorans in natural water microcosms
Pseudomonas oleovorans GPo1 and its polyhydroxyalkanoic acid (PHA) depolymerization-minus mutant, GPo500 phaZ, residing in natural water microcosms, were utilized to asses the effect of PHA availability on survival and resistance to stress agents. The wild-type strain showed increased survival compa...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00992240_v67_n1_p225_Ruiz |
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todo:paper_00992240_v67_n1_p225_Ruiz2023-10-03T14:57:05Z Polyhydroxyalkanoate degradation is associated with nucleotide accumulation and enhances stress resistance and survival of Pseudomonas oleovorans in natural water microcosms Ruiz, J.A. López, N.I. Fernández, R.O. Méndez, B.S. adenosine triphosphate guanosine 3' diphosphate 5' diphosphate nucleotide polyhydroxyalkanoic acid article bacterial growth degradation depolymerization gene mutation genetic complementation nonhuman oxidative phosphorylation phenotype Pseudomonas oleovorans stress survival Biodegradation, Environmental Carboxylic Ester Hydrolases Culture Media Ecosystem Ethanol Fresh Water Heat Mutation Nucleotides Polymers Pseudomonas Bacteria (microorganisms) Equidae Negibacteria Pseudomonas Pseudomonas oleovorans Pseudomonas oleovorans GPo1 and its polyhydroxyalkanoic acid (PHA) depolymerization-minus mutant, GPo500 phaZ, residing in natural water microcosms, were utilized to asses the effect of PHA availability on survival and resistance to stress agents. The wild-type strain showed increased survival compared to the PHA depolymerase-minus strain. The appearance of a round cellular shape, characteristic of bacteria growing under starvation conditions, was delayed in the wild type in comparison to the mutant strain. Percent survival at the end of ethanol and heat challenges was always higher in GPo1 than in GPo500. Based on these results and on early experiments (H. Hippe, Arch. Mikrobiol. 56:248-277, 1967) that suggested an association of PHA utilization with respiration and oxidative phosphorylation, we investigated the association between PHA degradation and nucleotide accumulation. ATP and guanosine tetraphosphate (ppGpp) production was analyzed under culture conditions leading to PHA depolymerization. A rise in the ATP and ppGpp levels appeared concomitant with PHA degradation, while this phenomenon was not observed in the mutant strain unable to degrade the polymer. Complementation of the phaZ mutation restored the wild-type phenotype. Fil:Ruiz, J.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Méndez, B.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00992240_v67_n1_p225_Ruiz |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
adenosine triphosphate guanosine 3' diphosphate 5' diphosphate nucleotide polyhydroxyalkanoic acid article bacterial growth degradation depolymerization gene mutation genetic complementation nonhuman oxidative phosphorylation phenotype Pseudomonas oleovorans stress survival Biodegradation, Environmental Carboxylic Ester Hydrolases Culture Media Ecosystem Ethanol Fresh Water Heat Mutation Nucleotides Polymers Pseudomonas Bacteria (microorganisms) Equidae Negibacteria Pseudomonas Pseudomonas oleovorans |
| spellingShingle |
adenosine triphosphate guanosine 3' diphosphate 5' diphosphate nucleotide polyhydroxyalkanoic acid article bacterial growth degradation depolymerization gene mutation genetic complementation nonhuman oxidative phosphorylation phenotype Pseudomonas oleovorans stress survival Biodegradation, Environmental Carboxylic Ester Hydrolases Culture Media Ecosystem Ethanol Fresh Water Heat Mutation Nucleotides Polymers Pseudomonas Bacteria (microorganisms) Equidae Negibacteria Pseudomonas Pseudomonas oleovorans Ruiz, J.A. López, N.I. Fernández, R.O. Méndez, B.S. Polyhydroxyalkanoate degradation is associated with nucleotide accumulation and enhances stress resistance and survival of Pseudomonas oleovorans in natural water microcosms |
| topic_facet |
adenosine triphosphate guanosine 3' diphosphate 5' diphosphate nucleotide polyhydroxyalkanoic acid article bacterial growth degradation depolymerization gene mutation genetic complementation nonhuman oxidative phosphorylation phenotype Pseudomonas oleovorans stress survival Biodegradation, Environmental Carboxylic Ester Hydrolases Culture Media Ecosystem Ethanol Fresh Water Heat Mutation Nucleotides Polymers Pseudomonas Bacteria (microorganisms) Equidae Negibacteria Pseudomonas Pseudomonas oleovorans |
| description |
Pseudomonas oleovorans GPo1 and its polyhydroxyalkanoic acid (PHA) depolymerization-minus mutant, GPo500 phaZ, residing in natural water microcosms, were utilized to asses the effect of PHA availability on survival and resistance to stress agents. The wild-type strain showed increased survival compared to the PHA depolymerase-minus strain. The appearance of a round cellular shape, characteristic of bacteria growing under starvation conditions, was delayed in the wild type in comparison to the mutant strain. Percent survival at the end of ethanol and heat challenges was always higher in GPo1 than in GPo500. Based on these results and on early experiments (H. Hippe, Arch. Mikrobiol. 56:248-277, 1967) that suggested an association of PHA utilization with respiration and oxidative phosphorylation, we investigated the association between PHA degradation and nucleotide accumulation. ATP and guanosine tetraphosphate (ppGpp) production was analyzed under culture conditions leading to PHA depolymerization. A rise in the ATP and ppGpp levels appeared concomitant with PHA degradation, while this phenomenon was not observed in the mutant strain unable to degrade the polymer. Complementation of the phaZ mutation restored the wild-type phenotype. |
| format |
JOUR |
| author |
Ruiz, J.A. López, N.I. Fernández, R.O. Méndez, B.S. |
| author_facet |
Ruiz, J.A. López, N.I. Fernández, R.O. Méndez, B.S. |
| author_sort |
Ruiz, J.A. |
| title |
Polyhydroxyalkanoate degradation is associated with nucleotide accumulation and enhances stress resistance and survival of Pseudomonas oleovorans in natural water microcosms |
| title_short |
Polyhydroxyalkanoate degradation is associated with nucleotide accumulation and enhances stress resistance and survival of Pseudomonas oleovorans in natural water microcosms |
| title_full |
Polyhydroxyalkanoate degradation is associated with nucleotide accumulation and enhances stress resistance and survival of Pseudomonas oleovorans in natural water microcosms |
| title_fullStr |
Polyhydroxyalkanoate degradation is associated with nucleotide accumulation and enhances stress resistance and survival of Pseudomonas oleovorans in natural water microcosms |
| title_full_unstemmed |
Polyhydroxyalkanoate degradation is associated with nucleotide accumulation and enhances stress resistance and survival of Pseudomonas oleovorans in natural water microcosms |
| title_sort |
polyhydroxyalkanoate degradation is associated with nucleotide accumulation and enhances stress resistance and survival of pseudomonas oleovorans in natural water microcosms |
| url |
http://hdl.handle.net/20.500.12110/paper_00992240_v67_n1_p225_Ruiz |
| work_keys_str_mv |
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| _version_ |
1807316004562272256 |