Tn 1000 (γδ) insertions stabilize pBR322-derived recombinant plasmids in Escherichia coli

Tn1000 (or 'γδ') insertions in a pBR322-recombinant plasmid carrying the threonine operon (pBE) were obtained either spontaneously during chemostat cultivation (pBE-2) or selected during transconjugation (pBE-3 and pBE-4). The different insertion derivatives were tested for their stability...

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Autores principales: Bellani, M., Shlain, V., Nudel, C., Sanchez-Rivas, C.
Formato: JOUR
Materias:
threonine
article
auxotrophy
chemostat
escherichia coli
gene insertion sequence
operon
recombinant plasmid
temperature
Escherichia coli
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_01415492_v19_n1_p1_Bellani
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_01415492_v19_n1_p1_Bellani
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spelling todo:paper_01415492_v19_n1_p1_Bellani2023-10-03T14:58:36Z Tn 1000 (γδ) insertions stabilize pBR322-derived recombinant plasmids in Escherichia coli Bellani, M. Shlain, V. Nudel, C. Sanchez-Rivas, C. threonine article auxotrophy chemostat escherichia coli gene insertion sequence operon recombinant plasmid temperature Escherichia coli Tn1000 (or 'γδ') insertions in a pBR322-recombinant plasmid carrying the threonine operon (pBE) were obtained either spontaneously during chemostat cultivation (pBE-2) or selected during transconjugation (pBE-3 and pBE-4). The different insertion derivatives were tested for their stability in different host genetic backgrounds (in relation to threonine auxotrophy and recA function), various media composition (liquid or solid, rich or minimal) and growth temperatures (30°C, 37°C and 42°C). All the derivatives carrying the γδ sequence, albeit increasing their size from 9.7 to 15.6 Kb, significantly enhanced segregational and structural plasmid stability in every condition tested. Fil:Bellani, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01415492_v19_n1_p1_Bellani
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic threonine
article
auxotrophy
chemostat
escherichia coli
gene insertion sequence
operon
recombinant plasmid
temperature
Escherichia coli
spellingShingle threonine
article
auxotrophy
chemostat
escherichia coli
gene insertion sequence
operon
recombinant plasmid
temperature
Escherichia coli
Bellani, M.
Shlain, V.
Nudel, C.
Sanchez-Rivas, C.
Tn 1000 (γδ) insertions stabilize pBR322-derived recombinant plasmids in Escherichia coli
topic_facet threonine
article
auxotrophy
chemostat
escherichia coli
gene insertion sequence
operon
recombinant plasmid
temperature
Escherichia coli
description Tn1000 (or 'γδ') insertions in a pBR322-recombinant plasmid carrying the threonine operon (pBE) were obtained either spontaneously during chemostat cultivation (pBE-2) or selected during transconjugation (pBE-3 and pBE-4). The different insertion derivatives were tested for their stability in different host genetic backgrounds (in relation to threonine auxotrophy and recA function), various media composition (liquid or solid, rich or minimal) and growth temperatures (30°C, 37°C and 42°C). All the derivatives carrying the γδ sequence, albeit increasing their size from 9.7 to 15.6 Kb, significantly enhanced segregational and structural plasmid stability in every condition tested.
format JOUR
author Bellani, M.
Shlain, V.
Nudel, C.
Sanchez-Rivas, C.
author_facet Bellani, M.
Shlain, V.
Nudel, C.
Sanchez-Rivas, C.
author_sort Bellani, M.
title Tn 1000 (γδ) insertions stabilize pBR322-derived recombinant plasmids in Escherichia coli
title_short Tn 1000 (γδ) insertions stabilize pBR322-derived recombinant plasmids in Escherichia coli
title_full Tn 1000 (γδ) insertions stabilize pBR322-derived recombinant plasmids in Escherichia coli
title_fullStr Tn 1000 (γδ) insertions stabilize pBR322-derived recombinant plasmids in Escherichia coli
title_full_unstemmed Tn 1000 (γδ) insertions stabilize pBR322-derived recombinant plasmids in Escherichia coli
title_sort tn 1000 (γδ) insertions stabilize pbr322-derived recombinant plasmids in escherichia coli
url http://hdl.handle.net/20.500.12110/paper_01415492_v19_n1_p1_Bellani
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