Tn 1000 (γδ) insertions stabilize pBR322-derived recombinant plasmids in Escherichia coli

Tn1000 (or 'γδ') insertions in a pBR322-recombinant plasmid carrying the threonine operon (pBE) were obtained either spontaneously during chemostat cultivation (pBE-2) or selected during transconjugation (pBE-3 and pBE-4). The different insertion derivatives were tested for their stability...

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Detalles Bibliográficos
Autores principales: Bellani, M., Shlain, V., Nudel, C., Sanchez-Rivas, C.
Formato: JOUR
Materias:
threonine
article
auxotrophy
chemostat
escherichia coli
gene insertion sequence
operon
recombinant plasmid
temperature
Escherichia coli
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_01415492_v19_n1_p1_Bellani
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:Tn1000 (or 'γδ') insertions in a pBR322-recombinant plasmid carrying the threonine operon (pBE) were obtained either spontaneously during chemostat cultivation (pBE-2) or selected during transconjugation (pBE-3 and pBE-4). The different insertion derivatives were tested for their stability in different host genetic backgrounds (in relation to threonine auxotrophy and recA function), various media composition (liquid or solid, rich or minimal) and growth temperatures (30°C, 37°C and 42°C). All the derivatives carrying the γδ sequence, albeit increasing their size from 9.7 to 15.6 Kb, significantly enhanced segregational and structural plasmid stability in every condition tested.

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