Site-specific recombination system from Tn 1000(γδ) stabilises recombinant plasmids in Escherichi coli

The resolution system from Tn 1000 was cloned into a pBR322 recombinant plasmid carrying E. coli threonine operon. The resulting plasmid displayed a significant increase in segregational stability with respect to the original plasmid, either in a rec+ or a rec- E. coli strain (100% of plasmid-contai...

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Autores principales: Bellani, M., Nudel, C., Sanchez Rivas, C.
Formato: JOUR
Materias:
threonine
article
cloning
escherichia coli
operon
recombinant plasmid
transposon
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_01415492_v19_n4_p331_Bellani
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_01415492_v19_n4_p331_Bellani
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spelling todo:paper_01415492_v19_n4_p331_Bellani2023-10-03T14:58:37Z Site-specific recombination system from Tn 1000(γδ) stabilises recombinant plasmids in Escherichi coli Bellani, M. Nudel, C. Sanchez Rivas, C. threonine article cloning escherichia coli operon recombinant plasmid transposon The resolution system from Tn 1000 was cloned into a pBR322 recombinant plasmid carrying E. coli threonine operon. The resulting plasmid displayed a significant increase in segregational stability with respect to the original plasmid, either in a rec+ or a rec- E. coli strain (100% of plasmid-containing cells versus 64% and 1% respectively). The Tn 1000 resolution system proved to be responsible for the stabilising effect exerted by the transposon when present in recombinant plasmids. Fil:Bellani, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01415492_v19_n4_p331_Bellani
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic threonine
article
cloning
escherichia coli
operon
recombinant plasmid
transposon
spellingShingle threonine
article
cloning
escherichia coli
operon
recombinant plasmid
transposon
Bellani, M.
Nudel, C.
Sanchez Rivas, C.
Site-specific recombination system from Tn 1000(γδ) stabilises recombinant plasmids in Escherichi coli
topic_facet threonine
article
cloning
escherichia coli
operon
recombinant plasmid
transposon
description The resolution system from Tn 1000 was cloned into a pBR322 recombinant plasmid carrying E. coli threonine operon. The resulting plasmid displayed a significant increase in segregational stability with respect to the original plasmid, either in a rec+ or a rec- E. coli strain (100% of plasmid-containing cells versus 64% and 1% respectively). The Tn 1000 resolution system proved to be responsible for the stabilising effect exerted by the transposon when present in recombinant plasmids.
format JOUR
author Bellani, M.
Nudel, C.
Sanchez Rivas, C.
author_facet Bellani, M.
Nudel, C.
Sanchez Rivas, C.
author_sort Bellani, M.
title Site-specific recombination system from Tn 1000(γδ) stabilises recombinant plasmids in Escherichi coli
title_short Site-specific recombination system from Tn 1000(γδ) stabilises recombinant plasmids in Escherichi coli
title_full Site-specific recombination system from Tn 1000(γδ) stabilises recombinant plasmids in Escherichi coli
title_fullStr Site-specific recombination system from Tn 1000(γδ) stabilises recombinant plasmids in Escherichi coli
title_full_unstemmed Site-specific recombination system from Tn 1000(γδ) stabilises recombinant plasmids in Escherichi coli
title_sort site-specific recombination system from tn 1000(γδ) stabilises recombinant plasmids in escherichi coli
url http://hdl.handle.net/20.500.12110/paper_01415492_v19_n4_p331_Bellani
work_keys_str_mv AT bellanim sitespecificrecombinationsystemfromtn1000gdstabilisesrecombinantplasmidsinescherichicoli
AT nudelc sitespecificrecombinationsystemfromtn1000gdstabilisesrecombinantplasmidsinescherichicoli
AT sanchezrivasc sitespecificrecombinationsystemfromtn1000gdstabilisesrecombinantplasmidsinescherichicoli
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