Chemical structure and anticoagulant activity of highly pyruvylated sulfated galactans from tropical green seaweeds of the order Bryopsidales
Sulfated and pyruvylated galactans were isolated from three tropical species of the Bryopsidales, Penicillus capitatus, Udotea flabellum, and Halimeda opuntia. They represent the only important sulfated polysaccharides present in the cell walls of these highly calcified seaweeds of the suborder Hali...
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todo:paper_01448617_v122_n_p376_Arata2023-10-03T14:59:32Z Chemical structure and anticoagulant activity of highly pyruvylated sulfated galactans from tropical green seaweeds of the order Bryopsidales Arata, P.X. Quintana, I. Canelón, D.J. Vera, B.E. Compagnone, R.S. Ciancia, M. Anticoagulant activity Fibrin formation Green seaweed Pyruvic acid ketal Sulfated galactan Nuclear magnetic resonance spectroscopy Polysaccharides Seaweed Anticoagulant activities Fibrin formation Green seaweed Pyruvic acid Sulfated galactans Chemical analysis Caulerpales Halimeda opuntia Penicillus capitatus Udotea flabellum anticoagulant agent calcium fibrin galactan polysaccharide pyruvic acid sulfate blood clotting Bryopsida cell wall chemistry drug effects green alga kinetics mass fragmentography metabolism nuclear magnetic resonance spectroscopy procedures seaweed Anticoagulants Blood Coagulation Bryopsida Calcium Cell Wall Chlorophyta Fibrin Galactans Gas Chromatography-Mass Spectrometry Kinetics Magnetic Resonance Spectroscopy Polysaccharides Pyruvic Acid Seaweed Sulfates Sulfated and pyruvylated galactans were isolated from three tropical species of the Bryopsidales, Penicillus capitatus, Udotea flabellum, and Halimeda opuntia. They represent the only important sulfated polysaccharides present in the cell walls of these highly calcified seaweeds of the suborder Halimedineae. Their structural features were studied by chemical analyses and NMR spectroscopy. Their backbone comprises 3-, 6-, and 3,6-linkages, constituted by major amounts of 3-linked 4,6-O-(1′-carboxy)ethylidene-d-galactopyranose units in part sulfated on C-2. Sulfation on C-2 was not found in galactans from other seaweeds of this order. In addition, a complex sulfation pattern, comprising also 4-, 6-, and 4,6-disulfated galactose units was found. A fraction from P. capitatus, F1, showed a moderate anticoagulant activity, evaluated by general coagulation tests and also kinetics of fibrin formation was assayed. Besides, preliminary results suggest that one of the possible mechanisms involved is direct thrombin inhibition. © 2014 Elsevier Ltd. All rights reserved. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01448617_v122_n_p376_Arata |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Anticoagulant activity Fibrin formation Green seaweed Pyruvic acid ketal Sulfated galactan Nuclear magnetic resonance spectroscopy Polysaccharides Seaweed Anticoagulant activities Fibrin formation Green seaweed Pyruvic acid Sulfated galactans Chemical analysis Caulerpales Halimeda opuntia Penicillus capitatus Udotea flabellum anticoagulant agent calcium fibrin galactan polysaccharide pyruvic acid sulfate blood clotting Bryopsida cell wall chemistry drug effects green alga kinetics mass fragmentography metabolism nuclear magnetic resonance spectroscopy procedures seaweed Anticoagulants Blood Coagulation Bryopsida Calcium Cell Wall Chlorophyta Fibrin Galactans Gas Chromatography-Mass Spectrometry Kinetics Magnetic Resonance Spectroscopy Polysaccharides Pyruvic Acid Seaweed Sulfates |
| spellingShingle |
Anticoagulant activity Fibrin formation Green seaweed Pyruvic acid ketal Sulfated galactan Nuclear magnetic resonance spectroscopy Polysaccharides Seaweed Anticoagulant activities Fibrin formation Green seaweed Pyruvic acid Sulfated galactans Chemical analysis Caulerpales Halimeda opuntia Penicillus capitatus Udotea flabellum anticoagulant agent calcium fibrin galactan polysaccharide pyruvic acid sulfate blood clotting Bryopsida cell wall chemistry drug effects green alga kinetics mass fragmentography metabolism nuclear magnetic resonance spectroscopy procedures seaweed Anticoagulants Blood Coagulation Bryopsida Calcium Cell Wall Chlorophyta Fibrin Galactans Gas Chromatography-Mass Spectrometry Kinetics Magnetic Resonance Spectroscopy Polysaccharides Pyruvic Acid Seaweed Sulfates Arata, P.X. Quintana, I. Canelón, D.J. Vera, B.E. Compagnone, R.S. Ciancia, M. Chemical structure and anticoagulant activity of highly pyruvylated sulfated galactans from tropical green seaweeds of the order Bryopsidales |
| topic_facet |
Anticoagulant activity Fibrin formation Green seaweed Pyruvic acid ketal Sulfated galactan Nuclear magnetic resonance spectroscopy Polysaccharides Seaweed Anticoagulant activities Fibrin formation Green seaweed Pyruvic acid Sulfated galactans Chemical analysis Caulerpales Halimeda opuntia Penicillus capitatus Udotea flabellum anticoagulant agent calcium fibrin galactan polysaccharide pyruvic acid sulfate blood clotting Bryopsida cell wall chemistry drug effects green alga kinetics mass fragmentography metabolism nuclear magnetic resonance spectroscopy procedures seaweed Anticoagulants Blood Coagulation Bryopsida Calcium Cell Wall Chlorophyta Fibrin Galactans Gas Chromatography-Mass Spectrometry Kinetics Magnetic Resonance Spectroscopy Polysaccharides Pyruvic Acid Seaweed Sulfates |
| description |
Sulfated and pyruvylated galactans were isolated from three tropical species of the Bryopsidales, Penicillus capitatus, Udotea flabellum, and Halimeda opuntia. They represent the only important sulfated polysaccharides present in the cell walls of these highly calcified seaweeds of the suborder Halimedineae. Their structural features were studied by chemical analyses and NMR spectroscopy. Their backbone comprises 3-, 6-, and 3,6-linkages, constituted by major amounts of 3-linked 4,6-O-(1′-carboxy)ethylidene-d-galactopyranose units in part sulfated on C-2. Sulfation on C-2 was not found in galactans from other seaweeds of this order. In addition, a complex sulfation pattern, comprising also 4-, 6-, and 4,6-disulfated galactose units was found. A fraction from P. capitatus, F1, showed a moderate anticoagulant activity, evaluated by general coagulation tests and also kinetics of fibrin formation was assayed. Besides, preliminary results suggest that one of the possible mechanisms involved is direct thrombin inhibition. © 2014 Elsevier Ltd. All rights reserved. |
| format |
JOUR |
| author |
Arata, P.X. Quintana, I. Canelón, D.J. Vera, B.E. Compagnone, R.S. Ciancia, M. |
| author_facet |
Arata, P.X. Quintana, I. Canelón, D.J. Vera, B.E. Compagnone, R.S. Ciancia, M. |
| author_sort |
Arata, P.X. |
| title |
Chemical structure and anticoagulant activity of highly pyruvylated sulfated galactans from tropical green seaweeds of the order Bryopsidales |
| title_short |
Chemical structure and anticoagulant activity of highly pyruvylated sulfated galactans from tropical green seaweeds of the order Bryopsidales |
| title_full |
Chemical structure and anticoagulant activity of highly pyruvylated sulfated galactans from tropical green seaweeds of the order Bryopsidales |
| title_fullStr |
Chemical structure and anticoagulant activity of highly pyruvylated sulfated galactans from tropical green seaweeds of the order Bryopsidales |
| title_full_unstemmed |
Chemical structure and anticoagulant activity of highly pyruvylated sulfated galactans from tropical green seaweeds of the order Bryopsidales |
| title_sort |
chemical structure and anticoagulant activity of highly pyruvylated sulfated galactans from tropical green seaweeds of the order bryopsidales |
| url |
http://hdl.handle.net/20.500.12110/paper_01448617_v122_n_p376_Arata |
| work_keys_str_mv |
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1807323169188478976 |