GABA uptake in a Saccharomyces cerevisiae strain.

The aim of this work was to characterize the 4-aminobutyric acid (GABA) transport in the Saccharomyces cerevisiae D27 strain, followed by the study of the relationship between 5-aminolevulinic acid (ALA) and GABA transport systems. It was found that the general amino acid permease (GAP) is not activ...

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Autores principales:	Bermúdez Moretti, M., Correa García, S., Ramos, E.H., Batlle, A.
Formato:	JOUR
Materias:	4 aminobutyric acid 4 aminobutyric acid carrier amino acid amino acid transporter aminolevulinic acid carrier protein GABA permease organic anion transporter proline transporter Saccharomyces cerevisiae protein UGA4 protein, S cerevisiae article comparative study drug effect growth, development and aging kinetics metabolism Saccharomyces cerevisiae species difference transport at the cellular level Amino Acid Transport Systems Amino Acid Transport Systems, Neutral Amino Acids Aminolevulinic Acid Biological Transport GABA Plasma Membrane Transport Proteins gamma-Aminobutyric Acid Kinetics Membrane Transport Proteins Organic Anion Transporters Saccharomyces cerevisiae Proteins Species Specificity
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_01455680_v41_n6_p843_BermudezMoretti
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_01455680_v41_n6_p843_BermudezMoretti
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spelling	todo:paper_01455680_v41_n6_p843_BermudezMoretti2023-10-03T14:59:55Z GABA uptake in a Saccharomyces cerevisiae strain. Bermúdez Moretti, M. Correa García, S. Ramos, E.H. Batlle, A. 4 aminobutyric acid 4 aminobutyric acid carrier amino acid amino acid transporter aminolevulinic acid carrier protein GABA permease organic anion transporter proline transporter Saccharomyces cerevisiae protein UGA4 protein, S cerevisiae article comparative study drug effect growth, development and aging kinetics metabolism Saccharomyces cerevisiae species difference transport at the cellular level Amino Acid Transport Systems Amino Acid Transport Systems, Neutral Amino Acids Aminolevulinic Acid Biological Transport GABA Plasma Membrane Transport Proteins gamma-Aminobutyric Acid Kinetics Membrane Transport Proteins Organic Anion Transporters Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Species Specificity The aim of this work was to characterize the 4-aminobutyric acid (GABA) transport in the Saccharomyces cerevisiae D27 strain, followed by the study of the relationship between 5-aminolevulinic acid (ALA) and GABA transport systems. It was found that the general amino acid permease (GAP) is not active in D27 strain, suggesting that GABA incorporation should be mediated by PUT4 and UGA4 permeases. However, after kinetic studies only one system was detected. It was also shown that GABA uptake is competitively inhibited by ALA. GABA incorporation is regulated by the carbon source but not by the nitrogen source. When cells were grown in the presence of GABA, its entrance was very low. Fil:Bermúdez Moretti, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Correa García, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ramos, E.H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Batlle, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01455680_v41_n6_p843_BermudezMoretti
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	4 aminobutyric acid 4 aminobutyric acid carrier amino acid amino acid transporter aminolevulinic acid carrier protein GABA permease organic anion transporter proline transporter Saccharomyces cerevisiae protein UGA4 protein, S cerevisiae article comparative study drug effect growth, development and aging kinetics metabolism Saccharomyces cerevisiae species difference transport at the cellular level Amino Acid Transport Systems Amino Acid Transport Systems, Neutral Amino Acids Aminolevulinic Acid Biological Transport GABA Plasma Membrane Transport Proteins gamma-Aminobutyric Acid Kinetics Membrane Transport Proteins Organic Anion Transporters Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Species Specificity
spellingShingle	4 aminobutyric acid 4 aminobutyric acid carrier amino acid amino acid transporter aminolevulinic acid carrier protein GABA permease organic anion transporter proline transporter Saccharomyces cerevisiae protein UGA4 protein, S cerevisiae article comparative study drug effect growth, development and aging kinetics metabolism Saccharomyces cerevisiae species difference transport at the cellular level Amino Acid Transport Systems Amino Acid Transport Systems, Neutral Amino Acids Aminolevulinic Acid Biological Transport GABA Plasma Membrane Transport Proteins gamma-Aminobutyric Acid Kinetics Membrane Transport Proteins Organic Anion Transporters Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Species Specificity Bermúdez Moretti, M. Correa García, S. Ramos, E.H. Batlle, A. GABA uptake in a Saccharomyces cerevisiae strain.
topic_facet	4 aminobutyric acid 4 aminobutyric acid carrier amino acid amino acid transporter aminolevulinic acid carrier protein GABA permease organic anion transporter proline transporter Saccharomyces cerevisiae protein UGA4 protein, S cerevisiae article comparative study drug effect growth, development and aging kinetics metabolism Saccharomyces cerevisiae species difference transport at the cellular level Amino Acid Transport Systems Amino Acid Transport Systems, Neutral Amino Acids Aminolevulinic Acid Biological Transport GABA Plasma Membrane Transport Proteins gamma-Aminobutyric Acid Kinetics Membrane Transport Proteins Organic Anion Transporters Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Species Specificity
description	The aim of this work was to characterize the 4-aminobutyric acid (GABA) transport in the Saccharomyces cerevisiae D27 strain, followed by the study of the relationship between 5-aminolevulinic acid (ALA) and GABA transport systems. It was found that the general amino acid permease (GAP) is not active in D27 strain, suggesting that GABA incorporation should be mediated by PUT4 and UGA4 permeases. However, after kinetic studies only one system was detected. It was also shown that GABA uptake is competitively inhibited by ALA. GABA incorporation is regulated by the carbon source but not by the nitrogen source. When cells were grown in the presence of GABA, its entrance was very low.
format	JOUR
author	Bermúdez Moretti, M. Correa García, S. Ramos, E.H. Batlle, A.
author_facet	Bermúdez Moretti, M. Correa García, S. Ramos, E.H. Batlle, A.
author_sort	Bermúdez Moretti, M.
title	GABA uptake in a Saccharomyces cerevisiae strain.
title_short	GABA uptake in a Saccharomyces cerevisiae strain.
title_full	GABA uptake in a Saccharomyces cerevisiae strain.
title_fullStr	GABA uptake in a Saccharomyces cerevisiae strain.
title_full_unstemmed	GABA uptake in a Saccharomyces cerevisiae strain.
title_sort	gaba uptake in a saccharomyces cerevisiae strain.
url	http://hdl.handle.net/20.500.12110/paper_01455680_v41_n6_p843_BermudezMoretti
work_keys_str_mv	AT bermudezmorettim gabauptakeinasaccharomycescerevisiaestrain AT correagarcias gabauptakeinasaccharomycescerevisiaestrain AT ramoseh gabauptakeinasaccharomycescerevisiaestrain AT batllea gabauptakeinasaccharomycescerevisiaestrain
_version_	1807321282718466048

GABA uptake in a Saccharomyces cerevisiae strain.

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