Analysis of primer independent phosphorylase activity in potato plants: high levels of activity in sink organs and sucrose-dependent activity in cultured stem explants.

One isoform of potato (Solanum tuberosum L., cv. Spunta), type L phosphorylase (EC 2.4.1.1), exhibiting primer independent activity appears to be tuber-specific. However, this activity can also be modulated by exogenous sucrose in storage as well as in non-storage organs. Primer independent phosphor...

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Autores principales:	Moreno, S., Tandecarz, J.S.
Formato:	JOUR
Materias:	isoenzyme phosphorylase starch sucrose article enzymology growth, development and aging metabolism potato tissue distribution Isoenzymes Phosphorylases Solanum tuberosum Starch Sucrose Tissue Distribution
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_01455680_v42_n5_p637_Moreno
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_01455680_v42_n5_p637_Moreno
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spelling	todo:paper_01455680_v42_n5_p637_Moreno2023-10-03T14:59:56Z Analysis of primer independent phosphorylase activity in potato plants: high levels of activity in sink organs and sucrose-dependent activity in cultured stem explants. Moreno, S. Tandecarz, J.S. isoenzyme phosphorylase starch sucrose article enzymology growth, development and aging metabolism potato tissue distribution Isoenzymes Phosphorylases Solanum tuberosum Starch Sucrose Tissue Distribution One isoform of potato (Solanum tuberosum L., cv. Spunta), type L phosphorylase (EC 2.4.1.1), exhibiting primer independent activity appears to be tuber-specific. However, this activity can also be modulated by exogenous sucrose in storage as well as in non-storage organs. Primer independent phosphorylase (PIPh) activity in microtubers and shoots of in vitro plantlets was found to be much higher than in tubers and shoots of soil-grown plants. Detached leaves of soil-grown plants showed an increase in PIPh activity as well when incubated in sucrose-containing Murashige-Skoog (MS) medium. This increase was always accompanied by a rise in starch content. The presence of metabolizable carbohydrates in the growth or incubation medium are likely to be responsible for the observed rise in PIPh activity. In vitro microtubers and micropropagated plantlet organs (shoots and roots) exhibited a correlation between measurable PIPh activity and presence of enzyme protein, as judged by Western blot analysis using anti-potato tuber type L phosphorylase antibody. Therefore, in addition, to be developmentally regulated (tuber-specific accumulation), PIPh activity associated with the tuber type L isoform might be under a form of metabolic regulation. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01455680_v42_n5_p637_Moreno
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	isoenzyme phosphorylase starch sucrose article enzymology growth, development and aging metabolism potato tissue distribution Isoenzymes Phosphorylases Solanum tuberosum Starch Sucrose Tissue Distribution
spellingShingle	isoenzyme phosphorylase starch sucrose article enzymology growth, development and aging metabolism potato tissue distribution Isoenzymes Phosphorylases Solanum tuberosum Starch Sucrose Tissue Distribution Moreno, S. Tandecarz, J.S. Analysis of primer independent phosphorylase activity in potato plants: high levels of activity in sink organs and sucrose-dependent activity in cultured stem explants.
topic_facet	isoenzyme phosphorylase starch sucrose article enzymology growth, development and aging metabolism potato tissue distribution Isoenzymes Phosphorylases Solanum tuberosum Starch Sucrose Tissue Distribution
description	One isoform of potato (Solanum tuberosum L., cv. Spunta), type L phosphorylase (EC 2.4.1.1), exhibiting primer independent activity appears to be tuber-specific. However, this activity can also be modulated by exogenous sucrose in storage as well as in non-storage organs. Primer independent phosphorylase (PIPh) activity in microtubers and shoots of in vitro plantlets was found to be much higher than in tubers and shoots of soil-grown plants. Detached leaves of soil-grown plants showed an increase in PIPh activity as well when incubated in sucrose-containing Murashige-Skoog (MS) medium. This increase was always accompanied by a rise in starch content. The presence of metabolizable carbohydrates in the growth or incubation medium are likely to be responsible for the observed rise in PIPh activity. In vitro microtubers and micropropagated plantlet organs (shoots and roots) exhibited a correlation between measurable PIPh activity and presence of enzyme protein, as judged by Western blot analysis using anti-potato tuber type L phosphorylase antibody. Therefore, in addition, to be developmentally regulated (tuber-specific accumulation), PIPh activity associated with the tuber type L isoform might be under a form of metabolic regulation.
format	JOUR
author	Moreno, S. Tandecarz, J.S.
author_facet	Moreno, S. Tandecarz, J.S.
author_sort	Moreno, S.
title	Analysis of primer independent phosphorylase activity in potato plants: high levels of activity in sink organs and sucrose-dependent activity in cultured stem explants.
title_short	Analysis of primer independent phosphorylase activity in potato plants: high levels of activity in sink organs and sucrose-dependent activity in cultured stem explants.
title_full	Analysis of primer independent phosphorylase activity in potato plants: high levels of activity in sink organs and sucrose-dependent activity in cultured stem explants.
title_fullStr	Analysis of primer independent phosphorylase activity in potato plants: high levels of activity in sink organs and sucrose-dependent activity in cultured stem explants.
title_full_unstemmed	Analysis of primer independent phosphorylase activity in potato plants: high levels of activity in sink organs and sucrose-dependent activity in cultured stem explants.
title_sort	analysis of primer independent phosphorylase activity in potato plants: high levels of activity in sink organs and sucrose-dependent activity in cultured stem explants.
url	http://hdl.handle.net/20.500.12110/paper_01455680_v42_n5_p637_Moreno
work_keys_str_mv	AT morenos analysisofprimerindependentphosphorylaseactivityinpotatoplantshighlevelsofactivityinsinkorgansandsucrosedependentactivityinculturedstemexplants AT tandecarzjs analysisofprimerindependentphosphorylaseactivityinpotatoplantshighlevelsofactivityinsinkorgansandsucrosedependentactivityinculturedstemexplants
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Analysis of primer independent phosphorylase activity in potato plants: high levels of activity in sink organs and sucrose-dependent activity in cultured stem explants.

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