Inhibition of UDP-glucose: protein transglucosylase by a maize endosperm protein factor.

UDP-glucose: protein transglucosylase (UPTG, EC 2.4.1.112) catalyzes the first step of protein-bound alpha-glucan synthesis in potato tuber and developing maize endosperm. The presence of a non-dialyzable, heat labile protein responsible for low levels of UPTG activity in developing maize endosperm...

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Autores principales: Rothschild, A., Wald, F.A., Bocca, S.N., Tandecarz, J.S.
Formato: JOUR
Materias:
alpha 1,4 glucan protein synthase (UDP forming)
alpha-1,4-glucan-protein synthase (UDP-forming)
enzyme inhibitor
glucosyltransferase
starch
vegetable protein
article
biosynthesis
drug antagonism
enzymology
growth, development and aging
isolation and purification
maize
metabolism
molecular weight
solubility
Enzyme Inhibitors
Glucosyltransferases
Molecular Weight
Plant Proteins
Solubility
Starch
Zea mays
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_01455680_v42_n5_p645_Rothschild
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_01455680_v42_n5_p645_Rothschild
record_format dspace
spelling todo:paper_01455680_v42_n5_p645_Rothschild2023-10-03T14:59:57Z Inhibition of UDP-glucose: protein transglucosylase by a maize endosperm protein factor. Rothschild, A. Wald, F.A. Bocca, S.N. Tandecarz, J.S. alpha 1,4 glucan protein synthase (UDP forming) alpha-1,4-glucan-protein synthase (UDP-forming) enzyme inhibitor glucosyltransferase starch vegetable protein article biosynthesis drug antagonism enzymology growth, development and aging isolation and purification maize metabolism molecular weight solubility Enzyme Inhibitors Glucosyltransferases Molecular Weight Plant Proteins Solubility Starch Zea mays UDP-glucose: protein transglucosylase (UPTG, EC 2.4.1.112) catalyzes the first step of protein-bound alpha-glucan synthesis in potato tuber and developing maize endosperm. The presence of a non-dialyzable, heat labile protein responsible for low levels of UPTG activity in developing maize endosperm was investigated. UPTG activity in 5-day old maize seedlings and potato tuber solubilized preparations was also reduced by the endosperm preparation. FPLC-Mono Q column chromatography of developing maize endosperm was effective in separating the inhibitor protein (IP) from UPTG. After gel filtration on Superose 12, IP yielded a major polypeptide of about 80 kDa on SDS-PAGE. IP was purified by gel filtration on Superose 12 and preparative SDS-PAGE, and specific antibodies were prepared. Polyclonal antibodies reacted specifically with an 80 kDa polypeptide of developing maize endosperm on Western blot. They also recognized a similar band in 5-day old maize seedlings, but not in potato tubers. The identification of a factor that regulates the level of UPTG activity in developing maize endosperm may help to elucidate the functional role of the enzyme in the initiation of starch synthesis during seed development. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01455680_v42_n5_p645_Rothschild
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic alpha 1,4 glucan protein synthase (UDP forming)
alpha-1,4-glucan-protein synthase (UDP-forming)
enzyme inhibitor
glucosyltransferase
starch
vegetable protein
article
biosynthesis
drug antagonism
enzymology
growth, development and aging
isolation and purification
maize
metabolism
molecular weight
solubility
Enzyme Inhibitors
Glucosyltransferases
Molecular Weight
Plant Proteins
Solubility
Starch
Zea mays
spellingShingle alpha 1,4 glucan protein synthase (UDP forming)
alpha-1,4-glucan-protein synthase (UDP-forming)
enzyme inhibitor
glucosyltransferase
starch
vegetable protein
article
biosynthesis
drug antagonism
enzymology
growth, development and aging
isolation and purification
maize
metabolism
molecular weight
solubility
Enzyme Inhibitors
Glucosyltransferases
Molecular Weight
Plant Proteins
Solubility
Starch
Zea mays
Rothschild, A.
Wald, F.A.
Bocca, S.N.
Tandecarz, J.S.
Inhibition of UDP-glucose: protein transglucosylase by a maize endosperm protein factor.
topic_facet alpha 1,4 glucan protein synthase (UDP forming)
alpha-1,4-glucan-protein synthase (UDP-forming)
enzyme inhibitor
glucosyltransferase
starch
vegetable protein
article
biosynthesis
drug antagonism
enzymology
growth, development and aging
isolation and purification
maize
metabolism
molecular weight
solubility
Enzyme Inhibitors
Glucosyltransferases
Molecular Weight
Plant Proteins
Solubility
Starch
Zea mays
description UDP-glucose: protein transglucosylase (UPTG, EC 2.4.1.112) catalyzes the first step of protein-bound alpha-glucan synthesis in potato tuber and developing maize endosperm. The presence of a non-dialyzable, heat labile protein responsible for low levels of UPTG activity in developing maize endosperm was investigated. UPTG activity in 5-day old maize seedlings and potato tuber solubilized preparations was also reduced by the endosperm preparation. FPLC-Mono Q column chromatography of developing maize endosperm was effective in separating the inhibitor protein (IP) from UPTG. After gel filtration on Superose 12, IP yielded a major polypeptide of about 80 kDa on SDS-PAGE. IP was purified by gel filtration on Superose 12 and preparative SDS-PAGE, and specific antibodies were prepared. Polyclonal antibodies reacted specifically with an 80 kDa polypeptide of developing maize endosperm on Western blot. They also recognized a similar band in 5-day old maize seedlings, but not in potato tubers. The identification of a factor that regulates the level of UPTG activity in developing maize endosperm may help to elucidate the functional role of the enzyme in the initiation of starch synthesis during seed development.
format JOUR
author Rothschild, A.
Wald, F.A.
Bocca, S.N.
Tandecarz, J.S.
author_facet Rothschild, A.
Wald, F.A.
Bocca, S.N.
Tandecarz, J.S.
author_sort Rothschild, A.
title Inhibition of UDP-glucose: protein transglucosylase by a maize endosperm protein factor.
title_short Inhibition of UDP-glucose: protein transglucosylase by a maize endosperm protein factor.
title_full Inhibition of UDP-glucose: protein transglucosylase by a maize endosperm protein factor.
title_fullStr Inhibition of UDP-glucose: protein transglucosylase by a maize endosperm protein factor.
title_full_unstemmed Inhibition of UDP-glucose: protein transglucosylase by a maize endosperm protein factor.
title_sort inhibition of udp-glucose: protein transglucosylase by a maize endosperm protein factor.
url http://hdl.handle.net/20.500.12110/paper_01455680_v42_n5_p645_Rothschild
work_keys_str_mv AT rothschilda inhibitionofudpglucoseproteintransglucosylasebyamaizeendospermproteinfactor
AT waldfa inhibitionofudpglucoseproteintransglucosylasebyamaizeendospermproteinfactor
AT boccasn inhibitionofudpglucoseproteintransglucosylasebyamaizeendospermproteinfactor
AT tandecarzjs inhibitionofudpglucoseproteintransglucosylasebyamaizeendospermproteinfactor
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