Trypanosoma cruzi's incorporation of [3H]-palmitic acid and [3H] -galactose into components shed by trypomastigotes

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Trypomastigotes were metabolically labeled with [3H]-palmitic acid or [3H] -galactose and labeled components were detected in the culture medium. Thin layer chromatography of the shed material showed several lipids in the [3H] -palmitic acid labeled sample while the sugar was mainly incorporated int...

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Autores principales:	Couto, A.S., Uhrig, M.L., Agusti, R., Befumo, M.F., Zingales, B., Colli, W., De Lederkremer, R.M.
Formato:	JOUR
Materias:	endogenous compound galactose glycoconjugate lipid membrane lipid palmitic acid parasite antigen palmitic acid derivative article cell culture cell surface culture medium macromolecule membrane vesicle nonhuman ph priority journal thin layer chromatography trypanosoma cruzi trypomastigote animal chemistry fractionation gel chromatography metabolism Trypanosoma cruzi Trypanosoma Animal Chemical Fractionation Chromatography, Gel Chromatography, Thin Layer Culture Media Galactose Glycoconjugates Lipids Palmitic Acid Palmitic Acids Support, Non-U.S. Gov't
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_01585231_v24_n6_p991_Couto
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_01585231_v24_n6_p991_Couto
record_format	dspace
spelling	todo:paper_01585231_v24_n6_p991_Couto2023-10-03T15:01:16Z Trypanosoma cruzi's incorporation of [3H]-palmitic acid and [3H] -galactose into components shed by trypomastigotes Couto, A.S. Uhrig, M.L. Agusti, R. Befumo, M.F. Zingales, B. Colli, W. De Lederkremer, R.M. endogenous compound galactose glycoconjugate lipid membrane lipid palmitic acid parasite antigen galactose glycoconjugate lipid palmitic acid derivative article cell culture cell surface culture medium macromolecule membrane vesicle nonhuman ph priority journal thin layer chromatography trypanosoma cruzi trypomastigote animal chemistry culture medium fractionation gel chromatography metabolism Trypanosoma cruzi Trypanosoma Trypanosoma cruzi Animal Chemical Fractionation Chromatography, Gel Chromatography, Thin Layer Culture Media Galactose Glycoconjugates Lipids Palmitic Acid Palmitic Acids Support, Non-U.S. Gov't Trypanosoma cruzi Trypomastigotes were metabolically labeled with [3H]-palmitic acid or [3H] -galactose and labeled components were detected in the culture medium. Thin layer chromatography of the shed material showed several lipids in the [3H] -palmitic acid labeled sample while the sugar was mainly incorporated into macromolecules. The material incorporated with the lipidic precursor was fractionated by DEAE-Sephadex (acetate form) and the amount of radioactivity was ten times higher in the acidic lipids than in the neutral lipids. When acidic lipids were further separated by Unisil, 73% of the radioactivity was recovered in the less polar fraction. Different patterns were obtained on comparison of the shed components with the lipids remaining in the parasite. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01585231_v24_n6_p991_Couto
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	endogenous compound galactose glycoconjugate lipid membrane lipid palmitic acid parasite antigen galactose glycoconjugate lipid palmitic acid derivative article cell culture cell surface culture medium macromolecule membrane vesicle nonhuman ph priority journal thin layer chromatography trypanosoma cruzi trypomastigote animal chemistry culture medium fractionation gel chromatography metabolism Trypanosoma cruzi Trypanosoma Trypanosoma cruzi Animal Chemical Fractionation Chromatography, Gel Chromatography, Thin Layer Culture Media Galactose Glycoconjugates Lipids Palmitic Acid Palmitic Acids Support, Non-U.S. Gov't Trypanosoma cruzi
spellingShingle	endogenous compound galactose glycoconjugate lipid membrane lipid palmitic acid parasite antigen galactose glycoconjugate lipid palmitic acid derivative article cell culture cell surface culture medium macromolecule membrane vesicle nonhuman ph priority journal thin layer chromatography trypanosoma cruzi trypomastigote animal chemistry culture medium fractionation gel chromatography metabolism Trypanosoma cruzi Trypanosoma Trypanosoma cruzi Animal Chemical Fractionation Chromatography, Gel Chromatography, Thin Layer Culture Media Galactose Glycoconjugates Lipids Palmitic Acid Palmitic Acids Support, Non-U.S. Gov't Trypanosoma cruzi Couto, A.S. Uhrig, M.L. Agusti, R. Befumo, M.F. Zingales, B. Colli, W. De Lederkremer, R.M. Trypanosoma cruzi's incorporation of [3H]-palmitic acid and [3H] -galactose into components shed by trypomastigotes
topic_facet	endogenous compound galactose glycoconjugate lipid membrane lipid palmitic acid parasite antigen galactose glycoconjugate lipid palmitic acid derivative article cell culture cell surface culture medium macromolecule membrane vesicle nonhuman ph priority journal thin layer chromatography trypanosoma cruzi trypomastigote animal chemistry culture medium fractionation gel chromatography metabolism Trypanosoma cruzi Trypanosoma Trypanosoma cruzi Animal Chemical Fractionation Chromatography, Gel Chromatography, Thin Layer Culture Media Galactose Glycoconjugates Lipids Palmitic Acid Palmitic Acids Support, Non-U.S. Gov't Trypanosoma cruzi
description	Trypomastigotes were metabolically labeled with [3H]-palmitic acid or [3H] -galactose and labeled components were detected in the culture medium. Thin layer chromatography of the shed material showed several lipids in the [3H] -palmitic acid labeled sample while the sugar was mainly incorporated into macromolecules. The material incorporated with the lipidic precursor was fractionated by DEAE-Sephadex (acetate form) and the amount of radioactivity was ten times higher in the acidic lipids than in the neutral lipids. When acidic lipids were further separated by Unisil, 73% of the radioactivity was recovered in the less polar fraction. Different patterns were obtained on comparison of the shed components with the lipids remaining in the parasite.
format	JOUR
author	Couto, A.S. Uhrig, M.L. Agusti, R. Befumo, M.F. Zingales, B. Colli, W. De Lederkremer, R.M.
author_facet	Couto, A.S. Uhrig, M.L. Agusti, R. Befumo, M.F. Zingales, B. Colli, W. De Lederkremer, R.M.
author_sort	Couto, A.S.
title	Trypanosoma cruzi's incorporation of [3H]-palmitic acid and [3H] -galactose into components shed by trypomastigotes
title_short	Trypanosoma cruzi's incorporation of [3H]-palmitic acid and [3H] -galactose into components shed by trypomastigotes
title_full	Trypanosoma cruzi's incorporation of [3H]-palmitic acid and [3H] -galactose into components shed by trypomastigotes
title_fullStr	Trypanosoma cruzi's incorporation of [3H]-palmitic acid and [3H] -galactose into components shed by trypomastigotes
title_full_unstemmed	Trypanosoma cruzi's incorporation of [3H]-palmitic acid and [3H] -galactose into components shed by trypomastigotes
title_sort	trypanosoma cruzi's incorporation of [3h]-palmitic acid and [3h] -galactose into components shed by trypomastigotes
url	http://hdl.handle.net/20.500.12110/paper_01585231_v24_n6_p991_Couto
work_keys_str_mv	AT coutoas trypanosomacruzisincorporationof3hpalmiticacidand3hgalactoseintocomponentsshedbytrypomastigotes AT uhrigml trypanosomacruzisincorporationof3hpalmiticacidand3hgalactoseintocomponentsshedbytrypomastigotes AT agustir trypanosomacruzisincorporationof3hpalmiticacidand3hgalactoseintocomponentsshedbytrypomastigotes AT befumomf trypanosomacruzisincorporationof3hpalmiticacidand3hgalactoseintocomponentsshedbytrypomastigotes AT zingalesb trypanosomacruzisincorporationof3hpalmiticacidand3hgalactoseintocomponentsshedbytrypomastigotes AT colliw trypanosomacruzisincorporationof3hpalmiticacidand3hgalactoseintocomponentsshedbytrypomastigotes AT delederkremerrm trypanosomacruzisincorporationof3hpalmiticacidand3hgalactoseintocomponentsshedbytrypomastigotes
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Trypanosoma cruzi's incorporation of [3H]-palmitic acid and [3H] -galactose into components shed by trypomastigotes

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