The secretome of Trametes versicolor grown on tomato juice medium and purification of the secreted oxidoreductases including a versatile peroxidase
The present work was carried out with the aim to analyze the secretome of Trametes versicolor BAFC 2234 grown on tomato juice medium supplemented with copper and manganese. T. versicolor BAFC 2234 was selected among diverse wood dwelling agaricomycetes from Argentina by its ability to cause a strong...
Guardado en:
| Autores principales: | , , , , , |
|---|---|
| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_01681656_v168_n1_p15_Carabajal |
| Aporte de: |
| id |
todo:paper_01681656_v168_n1_p15_Carabajal |
|---|---|
| record_format |
dspace |
| spelling |
todo:paper_01681656_v168_n1_p15_Carabajal2023-10-03T15:06:07Z The secretome of Trametes versicolor grown on tomato juice medium and purification of the secreted oxidoreductases including a versatile peroxidase Carabajal, M. Kellner, H. Levin, L. Jehmlich, N. Hofrichter, M. Ullrich, R. Laccase Manganese peroxidase Secretome Trametes versicolor Versatile peroxidase Laccases Manganese peroxidase Secretome Trametes versicolor Versatile peroxidase Biodegradation Fruits Hardwoods Lignin Proteins Purification Enzymes copper fungal protein hydrolase laccase lignocellulose manganese manganese peroxidase monophenol monooxygenase peroxidase phenol derivative secretome unclassified drug Argentina article carbon source culture medium degradation elution enzyme purification enzyme synthesis extracellular space fruit juice isoelectric point molecular weight nonhuman pH polyacrylamide gel electrophoresis priority journal protein analysis protein secretion tomato Trametes versicolor Biodegradation Enzymes Fruits Hardwoods Laccase Lignins Proteins Purification Laccase Manganese peroxidase Secretome Trametes versicolor Versatile peroxidase Culture Media Hydrolases Lycopersicon esculentum Monophenol Monooxygenase Oxidoreductases Peroxidase Trametes The present work was carried out with the aim to analyze the secretome of Trametes versicolor BAFC 2234 grown on tomato juice medium supplemented with copper and manganese. T. versicolor BAFC 2234 was selected among diverse wood dwelling agaricomycetes from Argentina by its ability to cause a strong white rot on hardwood and in addition to show high tolerance toward phenolic compounds. A considerable number of the identified proteins were related to the degradation/modification of lignocelluloses. Hydrolases, peroxidases and phenoloxidases were the most abundant enzymes produced under the above-mentioned culture conditions. The lignin-modifying oxidoreductases laccase, manganese peroxidase (MnP) and versatile peroxidase (VP) were successfully purified - the latter for the first time from T. versicolor. The native VP protein has a molecular mass of 45. kDa and an isoelectric point of pH 3.7. The study clearly shows that complex plant-based media being rich in phenolics, such as tomato juice, can stimulate the secretion of a broad set of extracellular lignocellulolytic enzymes. Using such natural products as fungal culture media may give the opportunity to investigate plant biomass decomposition as well as the biodegradation of organic pollutants in an environment close to nature. © 2013 Elsevier B.V. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01681656_v168_n1_p15_Carabajal |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Laccase Manganese peroxidase Secretome Trametes versicolor Versatile peroxidase Laccases Manganese peroxidase Secretome Trametes versicolor Versatile peroxidase Biodegradation Fruits Hardwoods Lignin Proteins Purification Enzymes copper fungal protein hydrolase laccase lignocellulose manganese manganese peroxidase monophenol monooxygenase peroxidase phenol derivative secretome unclassified drug Argentina article carbon source culture medium degradation elution enzyme purification enzyme synthesis extracellular space fruit juice isoelectric point molecular weight nonhuman pH polyacrylamide gel electrophoresis priority journal protein analysis protein secretion tomato Trametes versicolor Biodegradation Enzymes Fruits Hardwoods Laccase Lignins Proteins Purification Laccase Manganese peroxidase Secretome Trametes versicolor Versatile peroxidase Culture Media Hydrolases Lycopersicon esculentum Monophenol Monooxygenase Oxidoreductases Peroxidase Trametes |
| spellingShingle |
Laccase Manganese peroxidase Secretome Trametes versicolor Versatile peroxidase Laccases Manganese peroxidase Secretome Trametes versicolor Versatile peroxidase Biodegradation Fruits Hardwoods Lignin Proteins Purification Enzymes copper fungal protein hydrolase laccase lignocellulose manganese manganese peroxidase monophenol monooxygenase peroxidase phenol derivative secretome unclassified drug Argentina article carbon source culture medium degradation elution enzyme purification enzyme synthesis extracellular space fruit juice isoelectric point molecular weight nonhuman pH polyacrylamide gel electrophoresis priority journal protein analysis protein secretion tomato Trametes versicolor Biodegradation Enzymes Fruits Hardwoods Laccase Lignins Proteins Purification Laccase Manganese peroxidase Secretome Trametes versicolor Versatile peroxidase Culture Media Hydrolases Lycopersicon esculentum Monophenol Monooxygenase Oxidoreductases Peroxidase Trametes Carabajal, M. Kellner, H. Levin, L. Jehmlich, N. Hofrichter, M. Ullrich, R. The secretome of Trametes versicolor grown on tomato juice medium and purification of the secreted oxidoreductases including a versatile peroxidase |
| topic_facet |
Laccase Manganese peroxidase Secretome Trametes versicolor Versatile peroxidase Laccases Manganese peroxidase Secretome Trametes versicolor Versatile peroxidase Biodegradation Fruits Hardwoods Lignin Proteins Purification Enzymes copper fungal protein hydrolase laccase lignocellulose manganese manganese peroxidase monophenol monooxygenase peroxidase phenol derivative secretome unclassified drug Argentina article carbon source culture medium degradation elution enzyme purification enzyme synthesis extracellular space fruit juice isoelectric point molecular weight nonhuman pH polyacrylamide gel electrophoresis priority journal protein analysis protein secretion tomato Trametes versicolor Biodegradation Enzymes Fruits Hardwoods Laccase Lignins Proteins Purification Laccase Manganese peroxidase Secretome Trametes versicolor Versatile peroxidase Culture Media Hydrolases Lycopersicon esculentum Monophenol Monooxygenase Oxidoreductases Peroxidase Trametes |
| description |
The present work was carried out with the aim to analyze the secretome of Trametes versicolor BAFC 2234 grown on tomato juice medium supplemented with copper and manganese. T. versicolor BAFC 2234 was selected among diverse wood dwelling agaricomycetes from Argentina by its ability to cause a strong white rot on hardwood and in addition to show high tolerance toward phenolic compounds. A considerable number of the identified proteins were related to the degradation/modification of lignocelluloses. Hydrolases, peroxidases and phenoloxidases were the most abundant enzymes produced under the above-mentioned culture conditions. The lignin-modifying oxidoreductases laccase, manganese peroxidase (MnP) and versatile peroxidase (VP) were successfully purified - the latter for the first time from T. versicolor. The native VP protein has a molecular mass of 45. kDa and an isoelectric point of pH 3.7. The study clearly shows that complex plant-based media being rich in phenolics, such as tomato juice, can stimulate the secretion of a broad set of extracellular lignocellulolytic enzymes. Using such natural products as fungal culture media may give the opportunity to investigate plant biomass decomposition as well as the biodegradation of organic pollutants in an environment close to nature. © 2013 Elsevier B.V. |
| format |
JOUR |
| author |
Carabajal, M. Kellner, H. Levin, L. Jehmlich, N. Hofrichter, M. Ullrich, R. |
| author_facet |
Carabajal, M. Kellner, H. Levin, L. Jehmlich, N. Hofrichter, M. Ullrich, R. |
| author_sort |
Carabajal, M. |
| title |
The secretome of Trametes versicolor grown on tomato juice medium and purification of the secreted oxidoreductases including a versatile peroxidase |
| title_short |
The secretome of Trametes versicolor grown on tomato juice medium and purification of the secreted oxidoreductases including a versatile peroxidase |
| title_full |
The secretome of Trametes versicolor grown on tomato juice medium and purification of the secreted oxidoreductases including a versatile peroxidase |
| title_fullStr |
The secretome of Trametes versicolor grown on tomato juice medium and purification of the secreted oxidoreductases including a versatile peroxidase |
| title_full_unstemmed |
The secretome of Trametes versicolor grown on tomato juice medium and purification of the secreted oxidoreductases including a versatile peroxidase |
| title_sort |
secretome of trametes versicolor grown on tomato juice medium and purification of the secreted oxidoreductases including a versatile peroxidase |
| url |
http://hdl.handle.net/20.500.12110/paper_01681656_v168_n1_p15_Carabajal |
| work_keys_str_mv |
AT carabajalm thesecretomeoftrametesversicolorgrownontomatojuicemediumandpurificationofthesecretedoxidoreductasesincludingaversatileperoxidase AT kellnerh thesecretomeoftrametesversicolorgrownontomatojuicemediumandpurificationofthesecretedoxidoreductasesincludingaversatileperoxidase AT levinl thesecretomeoftrametesversicolorgrownontomatojuicemediumandpurificationofthesecretedoxidoreductasesincludingaversatileperoxidase AT jehmlichn thesecretomeoftrametesversicolorgrownontomatojuicemediumandpurificationofthesecretedoxidoreductasesincludingaversatileperoxidase AT hofrichterm thesecretomeoftrametesversicolorgrownontomatojuicemediumandpurificationofthesecretedoxidoreductasesincludingaversatileperoxidase AT ullrichr thesecretomeoftrametesversicolorgrownontomatojuicemediumandpurificationofthesecretedoxidoreductasesincludingaversatileperoxidase AT carabajalm secretomeoftrametesversicolorgrownontomatojuicemediumandpurificationofthesecretedoxidoreductasesincludingaversatileperoxidase AT kellnerh secretomeoftrametesversicolorgrownontomatojuicemediumandpurificationofthesecretedoxidoreductasesincludingaversatileperoxidase AT levinl secretomeoftrametesversicolorgrownontomatojuicemediumandpurificationofthesecretedoxidoreductasesincludingaversatileperoxidase AT jehmlichn secretomeoftrametesversicolorgrownontomatojuicemediumandpurificationofthesecretedoxidoreductasesincludingaversatileperoxidase AT hofrichterm secretomeoftrametesversicolorgrownontomatojuicemediumandpurificationofthesecretedoxidoreductasesincludingaversatileperoxidase AT ullrichr secretomeoftrametesversicolorgrownontomatojuicemediumandpurificationofthesecretedoxidoreductasesincludingaversatileperoxidase |
| _version_ |
1807314831672344576 |