Early activation of the mitochondrial apoptotic pathway in Vesicular Stomatitis Virus-infected cells
Vesicular Stomatitis Virus (VSV) has been shown to induce apoptosis in a caspase-dependent manner, but the precise apoptotic pathway remains unknown. We found that caspases 9 and 3, but not caspase 8, were activated during VSV-induced apoptosis in infected Vero cells. Since caspase 9 is related to t...
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todo:paper_01681702_v109_n1_p65_Gadaleta2023-10-03T15:06:08Z Early activation of the mitochondrial apoptotic pathway in Vesicular Stomatitis Virus-infected cells Gadaleta, P. Perfetti, X. Mersich, S. Coulombié, F. AIF Apoptosis Bcl-2 family proteins Caspases Cytochrome c Mitochondrial transmembrane potential VSV apoptosis inducing factor caspase 3 caspase 8 caspase 9 cytochrome c protein Bax protein bcl 2 protein bcl xl animal cell apoptosis article controlled study enzyme activation enzyme activity host pathogen interaction in vitro study membrane potential mitochondrial membrane mitochondrion nonhuman priority journal protein secretion RNA virus signal transduction Vero cell Vesicular stomatitis virus virus infection Vesicular stomatitis virus Vesicular Stomatitis Virus (VSV) has been shown to induce apoptosis in a caspase-dependent manner, but the precise apoptotic pathway remains unknown. We found that caspases 9 and 3, but not caspase 8, were activated during VSV-induced apoptosis in infected Vero cells. Since caspase 9 is related to the mitochondrial apoptotic pathway, we analyzed some mitochondrial events such as changes in the mitochondrial transmembrane potential (Δψm) and mitochondrial release of apoptogenic proteins such as cytochrome c and the apoptosis inducing factor (AIF). We found that VSV infection triggers the dissipation of the Δψm and the release of both cytochrome c and AIF from the mitochondrial intermembrane space very early in the VSV infection. These results indicate that the trigger of apoptosis in VSV-infected cells occurs through the early activation of the mitochondrial apoptotic pathway. On the other hand, intracellular levels of the anti-apoptotic proteins, such as Bcl-2 and Bcl-xL, and the pro-apoptotic protein Bax, were assessed during viral infection. These analyses showed that as viral infection proceeded, the cellular level of Bcl-xL decreased, while the levels of Bax and Bcl-2 remained unaffected. The significance of the Bcl-xL modulation is also discussed. © 2004 Elsevier B.V. All rights reserved. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01681702_v109_n1_p65_Gadaleta |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
AIF Apoptosis Bcl-2 family proteins Caspases Cytochrome c Mitochondrial transmembrane potential VSV apoptosis inducing factor caspase 3 caspase 8 caspase 9 cytochrome c protein Bax protein bcl 2 protein bcl xl animal cell apoptosis article controlled study enzyme activation enzyme activity host pathogen interaction in vitro study membrane potential mitochondrial membrane mitochondrion nonhuman priority journal protein secretion RNA virus signal transduction Vero cell Vesicular stomatitis virus virus infection Vesicular stomatitis virus |
| spellingShingle |
AIF Apoptosis Bcl-2 family proteins Caspases Cytochrome c Mitochondrial transmembrane potential VSV apoptosis inducing factor caspase 3 caspase 8 caspase 9 cytochrome c protein Bax protein bcl 2 protein bcl xl animal cell apoptosis article controlled study enzyme activation enzyme activity host pathogen interaction in vitro study membrane potential mitochondrial membrane mitochondrion nonhuman priority journal protein secretion RNA virus signal transduction Vero cell Vesicular stomatitis virus virus infection Vesicular stomatitis virus Gadaleta, P. Perfetti, X. Mersich, S. Coulombié, F. Early activation of the mitochondrial apoptotic pathway in Vesicular Stomatitis Virus-infected cells |
| topic_facet |
AIF Apoptosis Bcl-2 family proteins Caspases Cytochrome c Mitochondrial transmembrane potential VSV apoptosis inducing factor caspase 3 caspase 8 caspase 9 cytochrome c protein Bax protein bcl 2 protein bcl xl animal cell apoptosis article controlled study enzyme activation enzyme activity host pathogen interaction in vitro study membrane potential mitochondrial membrane mitochondrion nonhuman priority journal protein secretion RNA virus signal transduction Vero cell Vesicular stomatitis virus virus infection Vesicular stomatitis virus |
| description |
Vesicular Stomatitis Virus (VSV) has been shown to induce apoptosis in a caspase-dependent manner, but the precise apoptotic pathway remains unknown. We found that caspases 9 and 3, but not caspase 8, were activated during VSV-induced apoptosis in infected Vero cells. Since caspase 9 is related to the mitochondrial apoptotic pathway, we analyzed some mitochondrial events such as changes in the mitochondrial transmembrane potential (Δψm) and mitochondrial release of apoptogenic proteins such as cytochrome c and the apoptosis inducing factor (AIF). We found that VSV infection triggers the dissipation of the Δψm and the release of both cytochrome c and AIF from the mitochondrial intermembrane space very early in the VSV infection. These results indicate that the trigger of apoptosis in VSV-infected cells occurs through the early activation of the mitochondrial apoptotic pathway. On the other hand, intracellular levels of the anti-apoptotic proteins, such as Bcl-2 and Bcl-xL, and the pro-apoptotic protein Bax, were assessed during viral infection. These analyses showed that as viral infection proceeded, the cellular level of Bcl-xL decreased, while the levels of Bax and Bcl-2 remained unaffected. The significance of the Bcl-xL modulation is also discussed. © 2004 Elsevier B.V. All rights reserved. |
| format |
JOUR |
| author |
Gadaleta, P. Perfetti, X. Mersich, S. Coulombié, F. |
| author_facet |
Gadaleta, P. Perfetti, X. Mersich, S. Coulombié, F. |
| author_sort |
Gadaleta, P. |
| title |
Early activation of the mitochondrial apoptotic pathway in Vesicular Stomatitis Virus-infected cells |
| title_short |
Early activation of the mitochondrial apoptotic pathway in Vesicular Stomatitis Virus-infected cells |
| title_full |
Early activation of the mitochondrial apoptotic pathway in Vesicular Stomatitis Virus-infected cells |
| title_fullStr |
Early activation of the mitochondrial apoptotic pathway in Vesicular Stomatitis Virus-infected cells |
| title_full_unstemmed |
Early activation of the mitochondrial apoptotic pathway in Vesicular Stomatitis Virus-infected cells |
| title_sort |
early activation of the mitochondrial apoptotic pathway in vesicular stomatitis virus-infected cells |
| url |
http://hdl.handle.net/20.500.12110/paper_01681702_v109_n1_p65_Gadaleta |
| work_keys_str_mv |
AT gadaletap earlyactivationofthemitochondrialapoptoticpathwayinvesicularstomatitisvirusinfectedcells AT perfettix earlyactivationofthemitochondrialapoptoticpathwayinvesicularstomatitisvirusinfectedcells AT mersichs earlyactivationofthemitochondrialapoptoticpathwayinvesicularstomatitisvirusinfectedcells AT coulombief earlyactivationofthemitochondrialapoptoticpathwayinvesicularstomatitisvirusinfectedcells |
| _version_ |
1807314716614197248 |