Characterization of Junín virus particles inactivated by a zinc finger-reactive compound

Our previous studies reported the inhibitory action against arenaviruses of antiretroviral zinc finger-reactive compounds provided by the National Cancer Institute (USA). These compounds were able to inactivate virions as well as to reduce virus yields from infected cells. Here, the inactivation of...

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Autores principales:	García, C.C., Ellenberg, P.C., Artuso, M.C., Scolaro, L.A., Damonte, E.B.
Formato:	JOUR
Materias:	Arenavirus Junín virus Matrix protein Virus inactivation Zinc finger disulfide disulfide nsc20625 enhanced green fluorescent protein fatty acid binding protein hybrid protein matrix protein unclassified drug animal cell article cellular distribution controlled study electrophoresis endosome genetic transfection hemorrhagic fever Junin virus nonhuman priority journal virogenesis virus inactivation virus infectivity virus nucleocapsid virus particle zinc finger motif Animals Anti-HIV Agents Arenaviridae Infections Azo Compounds Carrier Proteins Cercopithecus aethiops Disulfides Green Fluorescent Proteins Microscopy, Electron, Transmission Nucleocapsid Proteins Recombinant Fusion Proteins Vero Cells Virion Virus Inactivation Zinc Fingers
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_01681702_v143_n1_p106_Garcia
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_01681702_v143_n1_p106_Garcia2023-10-03T15:06:11Z Characterization of Junín virus particles inactivated by a zinc finger-reactive compound García, C.C. Ellenberg, P.C. Artuso, M.C. Scolaro, L.A. Damonte, E.B. Arenavirus Junín virus Matrix protein Virus inactivation Zinc finger disulfide disulfide nsc20625 enhanced green fluorescent protein fatty acid binding protein hybrid protein matrix protein unclassified drug animal cell article cellular distribution controlled study electrophoresis endosome genetic transfection hemorrhagic fever Junin virus nonhuman priority journal virogenesis virus inactivation virus infectivity virus nucleocapsid virus particle zinc finger motif Animals Anti-HIV Agents Arenaviridae Infections Azo Compounds Carrier Proteins Cercopithecus aethiops Disulfides Green Fluorescent Proteins Junin virus Microscopy, Electron, Transmission Nucleocapsid Proteins Recombinant Fusion Proteins Vero Cells Virion Virus Inactivation Zinc Fingers Arenavirus Junin virus Our previous studies reported the inhibitory action against arenaviruses of antiretroviral zinc finger-reactive compounds provided by the National Cancer Institute (USA). These compounds were able to inactivate virions as well as to reduce virus yields from infected cells. Here, the inactivation of the arenavirus Junín (JUNV), agent of Argentine hemorrhagic fever, by the aromatic disulfide NSC20625 was analyzed. The treatment of purified JUNV with this compound eliminated infectivity apparently through irreversible modifications in the matrix Z protein detected by: (a) alterations in the electrophoretic migration profile of Z under non-reducing conditions; (b) an electrodense labeling in the internal layer beneath the envelope and around the matrix Z protein, in negatively stained preparations; (c) changes in the subcellular localization of Z in cells transfected with a recombinant fusion protein JUNVZ-eGFP. The infection of Vero cells with JUNV inactivated particles was blocked at the uncoating of viral nucleocapsid from endosomes, providing new evidence for a functional role of Z in this stage of arenavirus cycle. Furthermore, the inactivated JUNV particles retained the immunoreactivity of the surface glycoprotein GP1 suggesting that this disulfide may be useful in the pursuit of an inactivating agent to obtain a vaccine antigen or diagnostic tool. © 2009 Elsevier B.V. All rights reserved. Fil:García, C.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ellenberg, P.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Artuso, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Scolaro, L.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Damonte, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01681702_v143_n1_p106_Garcia
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Arenavirus Junín virus Matrix protein Virus inactivation Zinc finger disulfide disulfide nsc20625 enhanced green fluorescent protein fatty acid binding protein hybrid protein matrix protein unclassified drug animal cell article cellular distribution controlled study electrophoresis endosome genetic transfection hemorrhagic fever Junin virus nonhuman priority journal virogenesis virus inactivation virus infectivity virus nucleocapsid virus particle zinc finger motif Animals Anti-HIV Agents Arenaviridae Infections Azo Compounds Carrier Proteins Cercopithecus aethiops Disulfides Green Fluorescent Proteins Junin virus Microscopy, Electron, Transmission Nucleocapsid Proteins Recombinant Fusion Proteins Vero Cells Virion Virus Inactivation Zinc Fingers Arenavirus Junin virus
spellingShingle	Arenavirus Junín virus Matrix protein Virus inactivation Zinc finger disulfide disulfide nsc20625 enhanced green fluorescent protein fatty acid binding protein hybrid protein matrix protein unclassified drug animal cell article cellular distribution controlled study electrophoresis endosome genetic transfection hemorrhagic fever Junin virus nonhuman priority journal virogenesis virus inactivation virus infectivity virus nucleocapsid virus particle zinc finger motif Animals Anti-HIV Agents Arenaviridae Infections Azo Compounds Carrier Proteins Cercopithecus aethiops Disulfides Green Fluorescent Proteins Junin virus Microscopy, Electron, Transmission Nucleocapsid Proteins Recombinant Fusion Proteins Vero Cells Virion Virus Inactivation Zinc Fingers Arenavirus Junin virus García, C.C. Ellenberg, P.C. Artuso, M.C. Scolaro, L.A. Damonte, E.B. Characterization of Junín virus particles inactivated by a zinc finger-reactive compound
topic_facet	Arenavirus Junín virus Matrix protein Virus inactivation Zinc finger disulfide disulfide nsc20625 enhanced green fluorescent protein fatty acid binding protein hybrid protein matrix protein unclassified drug animal cell article cellular distribution controlled study electrophoresis endosome genetic transfection hemorrhagic fever Junin virus nonhuman priority journal virogenesis virus inactivation virus infectivity virus nucleocapsid virus particle zinc finger motif Animals Anti-HIV Agents Arenaviridae Infections Azo Compounds Carrier Proteins Cercopithecus aethiops Disulfides Green Fluorescent Proteins Junin virus Microscopy, Electron, Transmission Nucleocapsid Proteins Recombinant Fusion Proteins Vero Cells Virion Virus Inactivation Zinc Fingers Arenavirus Junin virus
description	Our previous studies reported the inhibitory action against arenaviruses of antiretroviral zinc finger-reactive compounds provided by the National Cancer Institute (USA). These compounds were able to inactivate virions as well as to reduce virus yields from infected cells. Here, the inactivation of the arenavirus Junín (JUNV), agent of Argentine hemorrhagic fever, by the aromatic disulfide NSC20625 was analyzed. The treatment of purified JUNV with this compound eliminated infectivity apparently through irreversible modifications in the matrix Z protein detected by: (a) alterations in the electrophoretic migration profile of Z under non-reducing conditions; (b) an electrodense labeling in the internal layer beneath the envelope and around the matrix Z protein, in negatively stained preparations; (c) changes in the subcellular localization of Z in cells transfected with a recombinant fusion protein JUNVZ-eGFP. The infection of Vero cells with JUNV inactivated particles was blocked at the uncoating of viral nucleocapsid from endosomes, providing new evidence for a functional role of Z in this stage of arenavirus cycle. Furthermore, the inactivated JUNV particles retained the immunoreactivity of the surface glycoprotein GP1 suggesting that this disulfide may be useful in the pursuit of an inactivating agent to obtain a vaccine antigen or diagnostic tool. © 2009 Elsevier B.V. All rights reserved.
format	JOUR
author	García, C.C. Ellenberg, P.C. Artuso, M.C. Scolaro, L.A. Damonte, E.B.
author_facet	García, C.C. Ellenberg, P.C. Artuso, M.C. Scolaro, L.A. Damonte, E.B.
author_sort	García, C.C.
title	Characterization of Junín virus particles inactivated by a zinc finger-reactive compound
title_short	Characterization of Junín virus particles inactivated by a zinc finger-reactive compound
title_full	Characterization of Junín virus particles inactivated by a zinc finger-reactive compound
title_fullStr	Characterization of Junín virus particles inactivated by a zinc finger-reactive compound
title_full_unstemmed	Characterization of Junín virus particles inactivated by a zinc finger-reactive compound
title_sort	characterization of junín virus particles inactivated by a zinc finger-reactive compound
url	http://hdl.handle.net/20.500.12110/paper_01681702_v143_n1_p106_Garcia
work_keys_str_mv	AT garciacc characterizationofjuninvirusparticlesinactivatedbyazincfingerreactivecompound AT ellenbergpc characterizationofjuninvirusparticlesinactivatedbyazincfingerreactivecompound AT artusomc characterizationofjuninvirusparticlesinactivatedbyazincfingerreactivecompound AT scolarola characterizationofjuninvirusparticlesinactivatedbyazincfingerreactivecompound AT damonteeb characterizationofjuninvirusparticlesinactivatedbyazincfingerreactivecompound
_version_	1807321412210262016

Characterization of Junín virus particles inactivated by a zinc finger-reactive compound

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