Relevance of number and sequence composition of knobs in the characterization of maize and teosinte

Knobs are heterochromatic blocks that can be found in 34 different chromosomal locations in maize (Zea mays ssp. mays) and teosintes (Zea mays ssp. parviglumis, Zea mays ssp. mexicana, Zea mays ssp. huehuetenanguensis, Zea luxurians, Zea nicaragüensis and Zea diploperennis). These are variable in nu...

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Autores principales:	González, G.E., Poggio, L., Fourastié, M.F.
Formato:	JOUR
Materias:	Cytogenetic characterization FISH. Knobs Maize Teosintes Zea
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_01877380_v36_n2_p127_Gonzalez
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_01877380_v36_n2_p127_Gonzalez
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spelling	todo:paper_01877380_v36_n2_p127_Gonzalez2023-10-03T15:08:49Z Relevance of number and sequence composition of knobs in the characterization of maize and teosinte González, G.E. Poggio, L. Fourastié, M.F. Cytogenetic characterization FISH. Knobs Maize Teosintes Zea Knobs are heterochromatic blocks that can be found in 34 different chromosomal locations in maize (Zea mays ssp. mays) and teosintes (Zea mays ssp. parviglumis, Zea mays ssp. mexicana, Zea mays ssp. huehuetenanguensis, Zea luxurians, Zea nicaragüensis and Zea diploperennis). These are variable in number, size and position among different accessions of maize and teosintes and can be detected by chromosome banding C and DAPI (4', 6'-diamidino-2-phenylindole), both in methaphases and in interphase nuclei. At a molecular level they are constituted by two families of sequences (180-bp and TR-1), and are represented by thousands to millions of copies, in different proportions to each other, forming the different types of knobs. In this study we analyzed if the variation in the number of knobs regions and the composition of their sequences allow the cytogenetic characterization of different maize and teosinte accessions. For this study, we applied classical (DAPI) and molecular (Fluorescence In Situ Hybridization-FISH) cytogenetic techniques. DAPI staining showed variations in the number of DAPI+ regions (knobs) on chromosomes and interphase nuclei. FISH showed co-localized hybridization signals with the DAPI+ regions; it also revealed variation of the sequences composition of knobs among the accessions tested. These results allowed finding differential hybridization patterns to discern different accessions of maize and teosintes herein studied, therefore providing data for cytogenetic characterization and contributing to the knowledge of the genetic variability of the genus Zea. Fil:González, G.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Poggio, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Fourastié, M.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01877380_v36_n2_p127_Gonzalez
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Cytogenetic characterization FISH. Knobs Maize Teosintes Zea
spellingShingle	Cytogenetic characterization FISH. Knobs Maize Teosintes Zea González, G.E. Poggio, L. Fourastié, M.F. Relevance of number and sequence composition of knobs in the characterization of maize and teosinte
topic_facet	Cytogenetic characterization FISH. Knobs Maize Teosintes Zea
description	Knobs are heterochromatic blocks that can be found in 34 different chromosomal locations in maize (Zea mays ssp. mays) and teosintes (Zea mays ssp. parviglumis, Zea mays ssp. mexicana, Zea mays ssp. huehuetenanguensis, Zea luxurians, Zea nicaragüensis and Zea diploperennis). These are variable in number, size and position among different accessions of maize and teosintes and can be detected by chromosome banding C and DAPI (4', 6'-diamidino-2-phenylindole), both in methaphases and in interphase nuclei. At a molecular level they are constituted by two families of sequences (180-bp and TR-1), and are represented by thousands to millions of copies, in different proportions to each other, forming the different types of knobs. In this study we analyzed if the variation in the number of knobs regions and the composition of their sequences allow the cytogenetic characterization of different maize and teosinte accessions. For this study, we applied classical (DAPI) and molecular (Fluorescence In Situ Hybridization-FISH) cytogenetic techniques. DAPI staining showed variations in the number of DAPI+ regions (knobs) on chromosomes and interphase nuclei. FISH showed co-localized hybridization signals with the DAPI+ regions; it also revealed variation of the sequences composition of knobs among the accessions tested. These results allowed finding differential hybridization patterns to discern different accessions of maize and teosintes herein studied, therefore providing data for cytogenetic characterization and contributing to the knowledge of the genetic variability of the genus Zea.
format	JOUR
author	González, G.E. Poggio, L. Fourastié, M.F.
author_facet	González, G.E. Poggio, L. Fourastié, M.F.
author_sort	González, G.E.
title	Relevance of number and sequence composition of knobs in the characterization of maize and teosinte
title_short	Relevance of number and sequence composition of knobs in the characterization of maize and teosinte
title_full	Relevance of number and sequence composition of knobs in the characterization of maize and teosinte
title_fullStr	Relevance of number and sequence composition of knobs in the characterization of maize and teosinte
title_full_unstemmed	Relevance of number and sequence composition of knobs in the characterization of maize and teosinte
title_sort	relevance of number and sequence composition of knobs in the characterization of maize and teosinte
url	http://hdl.handle.net/20.500.12110/paper_01877380_v36_n2_p127_Gonzalez
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Relevance of number and sequence composition of knobs in the characterization of maize and teosinte

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