Effects of exposure to nitrite on the antioxidant enzymes activity and the histopathological response of prawn Palaemonetes argentinus

The aim of the present study was to determine lethal and sublethal effects of nitrite on the freshwater prawn, Palaemonetes argentinus (Nobili 1901). The 24, 48, 72 and 96 h median lethal concentration (LC50) of nitrite were found to be 103.07; 91.94; 82.39 and 62.53 mg L-1, respectively. The antiox...

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Autores principales: Espino, M.L., Díaz, A.C., Velurtas, S.M.
Formato: JOUR
Materias:
Histology
LC50
Nitrite
Oxidative stress
Palaemonetes argentinus
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_01888897_v25_n1_p24_Espino
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_01888897_v25_n1_p24_Espino
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spelling todo:paper_01888897_v25_n1_p24_Espino2023-10-03T15:08:55Z Effects of exposure to nitrite on the antioxidant enzymes activity and the histopathological response of prawn Palaemonetes argentinus Espino, M.L. Díaz, A.C. Velurtas, S.M. Histology LC50 Nitrite Oxidative stress Palaemonetes argentinus The aim of the present study was to determine lethal and sublethal effects of nitrite on the freshwater prawn, Palaemonetes argentinus (Nobili 1901). The 24, 48, 72 and 96 h median lethal concentration (LC50) of nitrite were found to be 103.07; 91.94; 82.39 and 62.53 mg L-1, respectively. The antioxidant activity of superoxide dismutase (SOD) was positively correlated with the concentration of nitrite in the environment, thus increasing the formation of hydrogen peroxide, substrate of catalase (CAT), which also showed an increased activity under the same experimental conditions. Histological samples showed that nitrite exposure caused hyperplasia, necrosis and changes in the lamellar epithelium in gills; while the effects in the hepatopancreas were degenerative desquamation, epithelial deterioration and hyperplasia. In conclusion, nitrite toxicity in P. argentinus increases with time of exposure. Moreover, this study proves that histological analysis of crustacean gills and/or hepatopancreas is a useful tool to determine the presence of toxic concentrations of nitrite in the water. Additionally, the measurement of the activity of antioxidant enzymes (e.g., SOD and CAT) as possible indicators of nitrite pollution should be considered in future studies. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_01888897_v25_n1_p24_Espino
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Histology
LC50
Nitrite
Oxidative stress
Palaemonetes argentinus
spellingShingle Histology
LC50
Nitrite
Oxidative stress
Palaemonetes argentinus
Espino, M.L.
Díaz, A.C.
Velurtas, S.M.
Effects of exposure to nitrite on the antioxidant enzymes activity and the histopathological response of prawn Palaemonetes argentinus
topic_facet Histology
LC50
Nitrite
Oxidative stress
Palaemonetes argentinus
description The aim of the present study was to determine lethal and sublethal effects of nitrite on the freshwater prawn, Palaemonetes argentinus (Nobili 1901). The 24, 48, 72 and 96 h median lethal concentration (LC50) of nitrite were found to be 103.07; 91.94; 82.39 and 62.53 mg L-1, respectively. The antioxidant activity of superoxide dismutase (SOD) was positively correlated with the concentration of nitrite in the environment, thus increasing the formation of hydrogen peroxide, substrate of catalase (CAT), which also showed an increased activity under the same experimental conditions. Histological samples showed that nitrite exposure caused hyperplasia, necrosis and changes in the lamellar epithelium in gills; while the effects in the hepatopancreas were degenerative desquamation, epithelial deterioration and hyperplasia. In conclusion, nitrite toxicity in P. argentinus increases with time of exposure. Moreover, this study proves that histological analysis of crustacean gills and/or hepatopancreas is a useful tool to determine the presence of toxic concentrations of nitrite in the water. Additionally, the measurement of the activity of antioxidant enzymes (e.g., SOD and CAT) as possible indicators of nitrite pollution should be considered in future studies.
format JOUR
author Espino, M.L.
Díaz, A.C.
Velurtas, S.M.
author_facet Espino, M.L.
Díaz, A.C.
Velurtas, S.M.
author_sort Espino, M.L.
title Effects of exposure to nitrite on the antioxidant enzymes activity and the histopathological response of prawn Palaemonetes argentinus
title_short Effects of exposure to nitrite on the antioxidant enzymes activity and the histopathological response of prawn Palaemonetes argentinus
title_full Effects of exposure to nitrite on the antioxidant enzymes activity and the histopathological response of prawn Palaemonetes argentinus
title_fullStr Effects of exposure to nitrite on the antioxidant enzymes activity and the histopathological response of prawn Palaemonetes argentinus
title_full_unstemmed Effects of exposure to nitrite on the antioxidant enzymes activity and the histopathological response of prawn Palaemonetes argentinus
title_sort effects of exposure to nitrite on the antioxidant enzymes activity and the histopathological response of prawn palaemonetes argentinus
url http://hdl.handle.net/20.500.12110/paper_01888897_v25_n1_p24_Espino
work_keys_str_mv AT espinoml effectsofexposuretonitriteontheantioxidantenzymesactivityandthehistopathologicalresponseofprawnpalaemonetesargentinus
AT diazac effectsofexposuretonitriteontheantioxidantenzymesactivityandthehistopathologicalresponseofprawnpalaemonetesargentinus
AT velurtassm effectsofexposuretonitriteontheantioxidantenzymesactivityandthehistopathologicalresponseofprawnpalaemonetesargentinus
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