Acid Pullulanase from Bacillus polymyxa MIR-23

Within the frame of a screening program aimed at the isolation of amylolytic sporeformers, one strain with high amylolytic activity designated MIR-23 was selected. The microbial characterization was carried out by morphological and biochemical tests and, by means of statistical treatment, was identi...

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Autores principales: Castro, G.R., Santopietro, L.M.D., Siñeriz, F.
Formato: JOUR
Materias:
α-glucosidase
acidophilic microorganism
Bacillus polymyxa
batch culture
pullulanase, α-amylase
starch degradation
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_02732289_v37_n3_p227_Castro
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_02732289_v37_n3_p227_Castro
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spelling todo:paper_02732289_v37_n3_p227_Castro2023-10-03T15:15:50Z Acid Pullulanase from Bacillus polymyxa MIR-23 Castro, G.R. Santopietro, L.M.D. Siñeriz, F. α-glucosidase acidophilic microorganism Bacillus polymyxa batch culture pullulanase, α-amylase starch degradation Within the frame of a screening program aimed at the isolation of amylolytic sporeformers, one strain with high amylolytic activity designated MIR-23 was selected. The microbial characterization was carried out by morphological and biochemical tests and, by means of statistical treatment, was identified as Bacillus polymyxa. The organism could grow in acidic conditions (pH 5.0) on a starch medium and produce α-amylase, pullulanase, and α-glucosidase. Batch cultures showed the highest enzyme activities in the stationary phase. Pullulanase activity exhibited an optimal temperature of 52-57°C at pH 4.5-5.5. These properties would allow its use in the saccharification processes in the starch industries. © 1993 The Humana Press Inc. Fil:Castro, G.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Siñeriz, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_02732289_v37_n3_p227_Castro
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic α-glucosidase
acidophilic microorganism
Bacillus polymyxa
batch culture
pullulanase, α-amylase
starch degradation
spellingShingle α-glucosidase
acidophilic microorganism
Bacillus polymyxa
batch culture
pullulanase, α-amylase
starch degradation
Castro, G.R.
Santopietro, L.M.D.
Siñeriz, F.
Acid Pullulanase from Bacillus polymyxa MIR-23
topic_facet α-glucosidase
acidophilic microorganism
Bacillus polymyxa
batch culture
pullulanase, α-amylase
starch degradation
description Within the frame of a screening program aimed at the isolation of amylolytic sporeformers, one strain with high amylolytic activity designated MIR-23 was selected. The microbial characterization was carried out by morphological and biochemical tests and, by means of statistical treatment, was identified as Bacillus polymyxa. The organism could grow in acidic conditions (pH 5.0) on a starch medium and produce α-amylase, pullulanase, and α-glucosidase. Batch cultures showed the highest enzyme activities in the stationary phase. Pullulanase activity exhibited an optimal temperature of 52-57°C at pH 4.5-5.5. These properties would allow its use in the saccharification processes in the starch industries. © 1993 The Humana Press Inc.
format JOUR
author Castro, G.R.
Santopietro, L.M.D.
Siñeriz, F.
author_facet Castro, G.R.
Santopietro, L.M.D.
Siñeriz, F.
author_sort Castro, G.R.
title Acid Pullulanase from Bacillus polymyxa MIR-23
title_short Acid Pullulanase from Bacillus polymyxa MIR-23
title_full Acid Pullulanase from Bacillus polymyxa MIR-23
title_fullStr Acid Pullulanase from Bacillus polymyxa MIR-23
title_full_unstemmed Acid Pullulanase from Bacillus polymyxa MIR-23
title_sort acid pullulanase from bacillus polymyxa mir-23
url http://hdl.handle.net/20.500.12110/paper_02732289_v37_n3_p227_Castro
work_keys_str_mv AT castrogr acidpullulanasefrombacilluspolymyxamir23
AT santopietrolmd acidpullulanasefrombacilluspolymyxamir23
AT sinerizf acidpullulanasefrombacilluspolymyxamir23
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