Binding of estradiol receptor complexes to isolated human breast chromatin
The interaction of estradiol-receptor complexes and isolated human breast tumor chromatin was studied under equilibrium conditions. The estradiol-receptor complexes bound specifically to the chromatin of hormone dependent tumors and showed a single class of binding sites with a Ka of 0.96 × 1010m-1...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_03008177_v16_n2-3_p79_Charreau |
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todo:paper_03008177_v16_n2-3_p79_Charreau2023-10-03T15:17:45Z Binding of estradiol receptor complexes to isolated human breast chromatin Charreau, E.H. Baldi, A. albumin i 125 dna drug receptor estradiol receptor radioisotope breast breast carcinoma chromatin estradiol h 3 human normal human rna c 14 theoretical study Breast Neoplasms Chromatin DNA, Neoplasm Estradiol Female Human Kinetics Osmolar Concentration Receptors, Estrogen Sodium Chloride Temperature The interaction of estradiol-receptor complexes and isolated human breast tumor chromatin was studied under equilibrium conditions. The estradiol-receptor complexes bound specifically to the chromatin of hormone dependent tumors and showed a single class of binding sites with a Ka of 0.96 × 1010m-1 and a binding capacity of 1.5 pmoles/mg DNA. The binding was a temperature-dependent process and involved a "transformation" of the receptor protein. The heat-activated hormone-receptor com-plex was more active than the 8S form in the binding phenomenon. The specific interaction of estradiol-receptor complex with isolated chromatin was saturable and sensitive to conditions of temperature and ionic strength. Furthermore under optimal conditions no acceptor sites were detected in chromatin of hormone independent tumors. © 1977 Dr. W. Junk b.v. Publishers. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03008177_v16_n2-3_p79_Charreau |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
albumin i 125 dna drug receptor estradiol receptor radioisotope breast breast carcinoma chromatin estradiol h 3 human normal human rna c 14 theoretical study Breast Neoplasms Chromatin DNA, Neoplasm Estradiol Female Human Kinetics Osmolar Concentration Receptors, Estrogen Sodium Chloride Temperature |
| spellingShingle |
albumin i 125 dna drug receptor estradiol receptor radioisotope breast breast carcinoma chromatin estradiol h 3 human normal human rna c 14 theoretical study Breast Neoplasms Chromatin DNA, Neoplasm Estradiol Female Human Kinetics Osmolar Concentration Receptors, Estrogen Sodium Chloride Temperature Charreau, E.H. Baldi, A. Binding of estradiol receptor complexes to isolated human breast chromatin |
| topic_facet |
albumin i 125 dna drug receptor estradiol receptor radioisotope breast breast carcinoma chromatin estradiol h 3 human normal human rna c 14 theoretical study Breast Neoplasms Chromatin DNA, Neoplasm Estradiol Female Human Kinetics Osmolar Concentration Receptors, Estrogen Sodium Chloride Temperature |
| description |
The interaction of estradiol-receptor complexes and isolated human breast tumor chromatin was studied under equilibrium conditions. The estradiol-receptor complexes bound specifically to the chromatin of hormone dependent tumors and showed a single class of binding sites with a Ka of 0.96 × 1010m-1 and a binding capacity of 1.5 pmoles/mg DNA. The binding was a temperature-dependent process and involved a "transformation" of the receptor protein. The heat-activated hormone-receptor com-plex was more active than the 8S form in the binding phenomenon. The specific interaction of estradiol-receptor complex with isolated chromatin was saturable and sensitive to conditions of temperature and ionic strength. Furthermore under optimal conditions no acceptor sites were detected in chromatin of hormone independent tumors. © 1977 Dr. W. Junk b.v. Publishers. |
| format |
JOUR |
| author |
Charreau, E.H. Baldi, A. |
| author_facet |
Charreau, E.H. Baldi, A. |
| author_sort |
Charreau, E.H. |
| title |
Binding of estradiol receptor complexes to isolated human breast chromatin |
| title_short |
Binding of estradiol receptor complexes to isolated human breast chromatin |
| title_full |
Binding of estradiol receptor complexes to isolated human breast chromatin |
| title_fullStr |
Binding of estradiol receptor complexes to isolated human breast chromatin |
| title_full_unstemmed |
Binding of estradiol receptor complexes to isolated human breast chromatin |
| title_sort |
binding of estradiol receptor complexes to isolated human breast chromatin |
| url |
http://hdl.handle.net/20.500.12110/paper_03008177_v16_n2-3_p79_Charreau |
| work_keys_str_mv |
AT charreaueh bindingofestradiolreceptorcomplexestoisolatedhumanbreastchromatin AT baldia bindingofestradiolreceptorcomplexestoisolatedhumanbreastchromatin |
| _version_ |
1807321162355572736 |