Diversity of nitrifying bacteria in a full-scale petroleum refinery wastewater treatment plant experiencing unstable nitrification
We have investigated bacterial populations relevant to nitrification in a full-scale activated sludge plant receiving wastewater from a petroleum refinery showing unstable nitrification. Inhibition of ammonia oxidation was related to phenol concentration according to a model of non-competitive inhib...
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todo:paper_03043894_v181_n1-3_p281_Figuerola2023-10-03T15:20:13Z Diversity of nitrifying bacteria in a full-scale petroleum refinery wastewater treatment plant experiencing unstable nitrification Figuerola, E.L.M. Erijman, L. Activated sludge Ammonia-oxidizing bacteria Nitrification Nitrobacter Phenol 16S rRNA gene Activated sludge Activated sludge plants Ammonia oxidation Ammonia oxidizing bacteria Bacterial population Clone library Competitive inhibition Monooxygenases Nitrifying bacteria Nitrite accumulation Nitrite-oxidizing bacteria Nitrobacter Nitrosomonas Nitrosomonas europaea Nitrospira Nucleotide sequences Operational taxonomic units Phenol concentration Risk of failure Wastewater treatment plants Ammonia Bacteria Bacteriology Bioactivity Chemical oxygen demand Cloning Denitrification Nitration Oxidation Petroleum refineries Phenols Refining RNA Sewage pumping plants Wastewater Wastewater reclamation Wastewater treatment Water treatment plants Nitrification ammonia DNA nitrite petroleum phenol RNA 16S unspecific monooxygenase ammonia monooxygenase industrial waste oxidoreductase petroleum RNA 16S water pollutant activated sludge disturbance enzyme activity genetic analysis inhibition nitrification nitrifying bacterium oxidation petroleum phenol refining industry species diversity taxonomy waste facility wastewater water treatment ammonia oxidizing bacteria article DNA extraction gene sequence gene targeting microbial diversity molecular cloning nitrification nitrifying bacterium nitrite oxidizing bacterium Nitrobacter Nitrosomonas europaea nonhuman nucleotide sequence oxidation petrochemical industry phylogeny real time polymerase chain reaction sludge taxonomy unindexed sequence waste water management Bacteria bioremediation genetics industrial waste isolation and purification metabolism prevention and control procedures water management water pollutant Ammonia Bacteria Base Sequence Biodegradation, Environmental Industrial Waste Nitrites Oxidoreductases Petroleum RNA, Ribosomal, 16S Water Pollutants, Chemical Water Purification Bacteria (microorganisms) Nitrobacter Nitrosomonadales Nitrosomonas europaea Nitrosomonas nitrosa Nitrospira Ammonia Bacteria Base Sequence Biodegradation, Environmental Industrial Waste Nitrites Oxidoreductases Petroleum RNA, Ribosomal, 16S Water Pollutants, Chemical Water Purification We have investigated bacterial populations relevant to nitrification in a full-scale activated sludge plant receiving wastewater from a petroleum refinery showing unstable nitrification. Inhibition of ammonia oxidation was related to phenol concentration according to a model of non-competitive inhibition. While the number of ammonia-oxidizing bacteria (AOB) did not correlate with nitrification performance, the total number of nitrite-oxidizing bacteria (NOB) dropped considerably during periods of nitrite accumulation or no nitrification. Diversity of nitrifiers in the sludge of the full-scale facility was examined at a time of full nitrification with the construction of clone libraries of ammonia monooxygenase (amoA) gene and of the 16S rRNA gene of NOB. Nucleotide sequences of amoA gene belonged to one dominant population, associated with Nitrosomonas europaea, and to a minor population related to the Nitrosomonas nitrosa lineage. The majority of sequences retrieved in the NOB-like clone library also clustered within a single operational taxonomic unit. The high dominance of Nitrobacter over Nitrospira and the low diversity of nitrifying bacteria observed in this wastewater treatment plant might account for the increased risk of failure in the presence of disturbances. © 2010 Elsevier B.V. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03043894_v181_n1-3_p281_Figuerola |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Activated sludge Ammonia-oxidizing bacteria Nitrification Nitrobacter Phenol 16S rRNA gene Activated sludge Activated sludge plants Ammonia oxidation Ammonia oxidizing bacteria Bacterial population Clone library Competitive inhibition Monooxygenases Nitrifying bacteria Nitrite accumulation Nitrite-oxidizing bacteria Nitrobacter Nitrosomonas Nitrosomonas europaea Nitrospira Nucleotide sequences Operational taxonomic units Phenol concentration Risk of failure Wastewater treatment plants Ammonia Bacteria Bacteriology Bioactivity Chemical oxygen demand Cloning Denitrification Nitration Oxidation Petroleum refineries Phenols Refining RNA Sewage pumping plants Wastewater Wastewater reclamation Wastewater treatment Water treatment plants Nitrification ammonia DNA nitrite petroleum phenol RNA 16S unspecific monooxygenase ammonia monooxygenase industrial waste oxidoreductase petroleum RNA 16S water pollutant activated sludge disturbance enzyme activity genetic analysis inhibition nitrification nitrifying bacterium oxidation petroleum phenol refining industry species diversity taxonomy waste facility wastewater water treatment ammonia oxidizing bacteria article DNA extraction gene sequence gene targeting microbial diversity molecular cloning nitrification nitrifying bacterium nitrite oxidizing bacterium Nitrobacter Nitrosomonas europaea nonhuman nucleotide sequence oxidation petrochemical industry phylogeny real time polymerase chain reaction sludge taxonomy unindexed sequence waste water management Bacteria bioremediation genetics industrial waste isolation and purification metabolism prevention and control procedures water management water pollutant Ammonia Bacteria Base Sequence Biodegradation, Environmental Industrial Waste Nitrites Oxidoreductases Petroleum RNA, Ribosomal, 16S Water Pollutants, Chemical Water Purification Bacteria (microorganisms) Nitrobacter Nitrosomonadales Nitrosomonas europaea Nitrosomonas nitrosa Nitrospira Ammonia Bacteria Base Sequence Biodegradation, Environmental Industrial Waste Nitrites Oxidoreductases Petroleum RNA, Ribosomal, 16S Water Pollutants, Chemical Water Purification |
| spellingShingle |
Activated sludge Ammonia-oxidizing bacteria Nitrification Nitrobacter Phenol 16S rRNA gene Activated sludge Activated sludge plants Ammonia oxidation Ammonia oxidizing bacteria Bacterial population Clone library Competitive inhibition Monooxygenases Nitrifying bacteria Nitrite accumulation Nitrite-oxidizing bacteria Nitrobacter Nitrosomonas Nitrosomonas europaea Nitrospira Nucleotide sequences Operational taxonomic units Phenol concentration Risk of failure Wastewater treatment plants Ammonia Bacteria Bacteriology Bioactivity Chemical oxygen demand Cloning Denitrification Nitration Oxidation Petroleum refineries Phenols Refining RNA Sewage pumping plants Wastewater Wastewater reclamation Wastewater treatment Water treatment plants Nitrification ammonia DNA nitrite petroleum phenol RNA 16S unspecific monooxygenase ammonia monooxygenase industrial waste oxidoreductase petroleum RNA 16S water pollutant activated sludge disturbance enzyme activity genetic analysis inhibition nitrification nitrifying bacterium oxidation petroleum phenol refining industry species diversity taxonomy waste facility wastewater water treatment ammonia oxidizing bacteria article DNA extraction gene sequence gene targeting microbial diversity molecular cloning nitrification nitrifying bacterium nitrite oxidizing bacterium Nitrobacter Nitrosomonas europaea nonhuman nucleotide sequence oxidation petrochemical industry phylogeny real time polymerase chain reaction sludge taxonomy unindexed sequence waste water management Bacteria bioremediation genetics industrial waste isolation and purification metabolism prevention and control procedures water management water pollutant Ammonia Bacteria Base Sequence Biodegradation, Environmental Industrial Waste Nitrites Oxidoreductases Petroleum RNA, Ribosomal, 16S Water Pollutants, Chemical Water Purification Bacteria (microorganisms) Nitrobacter Nitrosomonadales Nitrosomonas europaea Nitrosomonas nitrosa Nitrospira Ammonia Bacteria Base Sequence Biodegradation, Environmental Industrial Waste Nitrites Oxidoreductases Petroleum RNA, Ribosomal, 16S Water Pollutants, Chemical Water Purification Figuerola, E.L.M. Erijman, L. Diversity of nitrifying bacteria in a full-scale petroleum refinery wastewater treatment plant experiencing unstable nitrification |
| topic_facet |
Activated sludge Ammonia-oxidizing bacteria Nitrification Nitrobacter Phenol 16S rRNA gene Activated sludge Activated sludge plants Ammonia oxidation Ammonia oxidizing bacteria Bacterial population Clone library Competitive inhibition Monooxygenases Nitrifying bacteria Nitrite accumulation Nitrite-oxidizing bacteria Nitrobacter Nitrosomonas Nitrosomonas europaea Nitrospira Nucleotide sequences Operational taxonomic units Phenol concentration Risk of failure Wastewater treatment plants Ammonia Bacteria Bacteriology Bioactivity Chemical oxygen demand Cloning Denitrification Nitration Oxidation Petroleum refineries Phenols Refining RNA Sewage pumping plants Wastewater Wastewater reclamation Wastewater treatment Water treatment plants Nitrification ammonia DNA nitrite petroleum phenol RNA 16S unspecific monooxygenase ammonia monooxygenase industrial waste oxidoreductase petroleum RNA 16S water pollutant activated sludge disturbance enzyme activity genetic analysis inhibition nitrification nitrifying bacterium oxidation petroleum phenol refining industry species diversity taxonomy waste facility wastewater water treatment ammonia oxidizing bacteria article DNA extraction gene sequence gene targeting microbial diversity molecular cloning nitrification nitrifying bacterium nitrite oxidizing bacterium Nitrobacter Nitrosomonas europaea nonhuman nucleotide sequence oxidation petrochemical industry phylogeny real time polymerase chain reaction sludge taxonomy unindexed sequence waste water management Bacteria bioremediation genetics industrial waste isolation and purification metabolism prevention and control procedures water management water pollutant Ammonia Bacteria Base Sequence Biodegradation, Environmental Industrial Waste Nitrites Oxidoreductases Petroleum RNA, Ribosomal, 16S Water Pollutants, Chemical Water Purification Bacteria (microorganisms) Nitrobacter Nitrosomonadales Nitrosomonas europaea Nitrosomonas nitrosa Nitrospira Ammonia Bacteria Base Sequence Biodegradation, Environmental Industrial Waste Nitrites Oxidoreductases Petroleum RNA, Ribosomal, 16S Water Pollutants, Chemical Water Purification |
| description |
We have investigated bacterial populations relevant to nitrification in a full-scale activated sludge plant receiving wastewater from a petroleum refinery showing unstable nitrification. Inhibition of ammonia oxidation was related to phenol concentration according to a model of non-competitive inhibition. While the number of ammonia-oxidizing bacteria (AOB) did not correlate with nitrification performance, the total number of nitrite-oxidizing bacteria (NOB) dropped considerably during periods of nitrite accumulation or no nitrification. Diversity of nitrifiers in the sludge of the full-scale facility was examined at a time of full nitrification with the construction of clone libraries of ammonia monooxygenase (amoA) gene and of the 16S rRNA gene of NOB. Nucleotide sequences of amoA gene belonged to one dominant population, associated with Nitrosomonas europaea, and to a minor population related to the Nitrosomonas nitrosa lineage. The majority of sequences retrieved in the NOB-like clone library also clustered within a single operational taxonomic unit. The high dominance of Nitrobacter over Nitrospira and the low diversity of nitrifying bacteria observed in this wastewater treatment plant might account for the increased risk of failure in the presence of disturbances. © 2010 Elsevier B.V. |
| format |
JOUR |
| author |
Figuerola, E.L.M. Erijman, L. |
| author_facet |
Figuerola, E.L.M. Erijman, L. |
| author_sort |
Figuerola, E.L.M. |
| title |
Diversity of nitrifying bacteria in a full-scale petroleum refinery wastewater treatment plant experiencing unstable nitrification |
| title_short |
Diversity of nitrifying bacteria in a full-scale petroleum refinery wastewater treatment plant experiencing unstable nitrification |
| title_full |
Diversity of nitrifying bacteria in a full-scale petroleum refinery wastewater treatment plant experiencing unstable nitrification |
| title_fullStr |
Diversity of nitrifying bacteria in a full-scale petroleum refinery wastewater treatment plant experiencing unstable nitrification |
| title_full_unstemmed |
Diversity of nitrifying bacteria in a full-scale petroleum refinery wastewater treatment plant experiencing unstable nitrification |
| title_sort |
diversity of nitrifying bacteria in a full-scale petroleum refinery wastewater treatment plant experiencing unstable nitrification |
| url |
http://hdl.handle.net/20.500.12110/paper_03043894_v181_n1-3_p281_Figuerola |
| work_keys_str_mv |
AT figuerolaelm diversityofnitrifyingbacteriainafullscalepetroleumrefinerywastewatertreatmentplantexperiencingunstablenitrification AT erijmanl diversityofnitrifyingbacteriainafullscalepetroleumrefinerywastewatertreatmentplantexperiencingunstablenitrification |
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1807318396423897088 |