Quantitation of polyamines in hypothalamus and pituitary of female and male developing rats

The quantitation of four polyamines in hypothalamus and pituitary is studied in male and female developing rats using an improved high-performance liquid chromatography method. In the hypothalamus, putrescine (PUT) reaches the highest concentration (nmol/mg protein) on day 6. It shows the lowest val...

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Autores principales: Thyssen, S.M., Libertun, C.
Formato: JOUR
Materias:
Development
Hypothalamus
Pituitary
Polyamines
Rat
Sexes
α-Difluoromethylornithine
agmatine
eflornithine
ornithine decarboxylase inhibitor
polyamine derivative
putrescine
spermidine
animal experiment
article
controlled study
diagnostic value
dose response
female
high performance liquid chromatography
hypophysis
hypophysis function
hypothalamus
male
mammal
nonhuman
perinatal period
priority journal
quantitative analysis
rat
reproduction
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03043940_v323_n1_p65_Thyssen
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_03043940_v323_n1_p65_Thyssen
record_format dspace
spelling todo:paper_03043940_v323_n1_p65_Thyssen2023-10-03T15:20:18Z Quantitation of polyamines in hypothalamus and pituitary of female and male developing rats Thyssen, S.M. Libertun, C. Development Hypothalamus Pituitary Polyamines Rat Sexes α-Difluoromethylornithine agmatine eflornithine ornithine decarboxylase inhibitor polyamine derivative putrescine spermidine animal experiment article controlled study diagnostic value dose response female high performance liquid chromatography hypophysis hypophysis function hypothalamus male mammal nonhuman perinatal period priority journal quantitative analysis rat reproduction The quantitation of four polyamines in hypothalamus and pituitary is studied in male and female developing rats using an improved high-performance liquid chromatography method. In the hypothalamus, putrescine (PUT) reaches the highest concentration (nmol/mg protein) on day 6. It shows the lowest value in comparison with any other polyamine. Spermidine (SPD) is high during the first postnatal days. Spermine (SPM) fluctuates, and agmatine (AGM) is highest during the first week. SPD, SPM and AGM are lower in females. In the pituitary, PUT, SPD and AGM are high during the first week. SPM remains constant and it is higher in males. AGM is higher in males only on day 1. PUT shows the lowest concentration of all. Concentrations of PUT, SPD and SPM are higher in the pituitary; AGM is higher in the hypothalamus. α-Difluoromethylornithine (a specific and irreversible inhibitor of ornithine decarboxylase) decreases PUT and SPD, increased SPM and AGM remain unchanged in the hypothalamus and pituitary. Thus, each polyamine has its own pattern in hypothalamus and in pituitary during development in males and females; these changes could be related to the hypothalamic control of pituitary secretion of hormones related to reproduction in mammals. © 2002 Elsevier Science Ireland Ltd. All rights reserved. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03043940_v323_n1_p65_Thyssen
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Development
Hypothalamus
Pituitary
Polyamines
Rat
Sexes
α-Difluoromethylornithine
agmatine
eflornithine
ornithine decarboxylase inhibitor
polyamine derivative
putrescine
spermidine
animal experiment
article
controlled study
diagnostic value
dose response
female
high performance liquid chromatography
hypophysis
hypophysis function
hypothalamus
male
mammal
nonhuman
perinatal period
priority journal
quantitative analysis
rat
reproduction
spellingShingle Development
Hypothalamus
Pituitary
Polyamines
Rat
Sexes
α-Difluoromethylornithine
agmatine
eflornithine
ornithine decarboxylase inhibitor
polyamine derivative
putrescine
spermidine
animal experiment
article
controlled study
diagnostic value
dose response
female
high performance liquid chromatography
hypophysis
hypophysis function
hypothalamus
male
mammal
nonhuman
perinatal period
priority journal
quantitative analysis
rat
reproduction
Thyssen, S.M.
Libertun, C.
Quantitation of polyamines in hypothalamus and pituitary of female and male developing rats
topic_facet Development
Hypothalamus
Pituitary
Polyamines
Rat
Sexes
α-Difluoromethylornithine
agmatine
eflornithine
ornithine decarboxylase inhibitor
polyamine derivative
putrescine
spermidine
animal experiment
article
controlled study
diagnostic value
dose response
female
high performance liquid chromatography
hypophysis
hypophysis function
hypothalamus
male
mammal
nonhuman
perinatal period
priority journal
quantitative analysis
rat
reproduction
description The quantitation of four polyamines in hypothalamus and pituitary is studied in male and female developing rats using an improved high-performance liquid chromatography method. In the hypothalamus, putrescine (PUT) reaches the highest concentration (nmol/mg protein) on day 6. It shows the lowest value in comparison with any other polyamine. Spermidine (SPD) is high during the first postnatal days. Spermine (SPM) fluctuates, and agmatine (AGM) is highest during the first week. SPD, SPM and AGM are lower in females. In the pituitary, PUT, SPD and AGM are high during the first week. SPM remains constant and it is higher in males. AGM is higher in males only on day 1. PUT shows the lowest concentration of all. Concentrations of PUT, SPD and SPM are higher in the pituitary; AGM is higher in the hypothalamus. α-Difluoromethylornithine (a specific and irreversible inhibitor of ornithine decarboxylase) decreases PUT and SPD, increased SPM and AGM remain unchanged in the hypothalamus and pituitary. Thus, each polyamine has its own pattern in hypothalamus and in pituitary during development in males and females; these changes could be related to the hypothalamic control of pituitary secretion of hormones related to reproduction in mammals. © 2002 Elsevier Science Ireland Ltd. All rights reserved.
format JOUR
author Thyssen, S.M.
Libertun, C.
author_facet Thyssen, S.M.
Libertun, C.
author_sort Thyssen, S.M.
title Quantitation of polyamines in hypothalamus and pituitary of female and male developing rats
title_short Quantitation of polyamines in hypothalamus and pituitary of female and male developing rats
title_full Quantitation of polyamines in hypothalamus and pituitary of female and male developing rats
title_fullStr Quantitation of polyamines in hypothalamus and pituitary of female and male developing rats
title_full_unstemmed Quantitation of polyamines in hypothalamus and pituitary of female and male developing rats
title_sort quantitation of polyamines in hypothalamus and pituitary of female and male developing rats
url http://hdl.handle.net/20.500.12110/paper_03043940_v323_n1_p65_Thyssen
work_keys_str_mv AT thyssensm quantitationofpolyaminesinhypothalamusandpituitaryoffemaleandmaledevelopingrats
AT libertunc quantitationofpolyaminesinhypothalamusandpituitaryoffemaleandmaledevelopingrats
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