Porphyrin biosynthesis. IV. 5- and 6-COOH porphyrinogens (type III) as normal intermediates in haem biosynthesis
1. 1. The role of 5- and 6-COOH porphyrinogens III in haem biosynthesis was studied in vitro. 2. 2. When haemolysates of normal chicken erythrocytes were incubated in the presence of 5- or 6-COOH porphyrinogens III, a marked isotopic dilution was observed served on the [2-12C]glycine incorporation i...
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todo:paper_03044165_v158_n1_p79_SanMartinDeViale2023-10-03T15:20:42Z Porphyrin biosynthesis. IV. 5- and 6-COOH porphyrinogens (type III) as normal intermediates in haem biosynthesis San Martín De Viale, L.C. Grinstein, M. Grinstein, M. carbon glycine heme porphyrin animal article biosynthesis chicken enzymology erythrocyte female hemolysis male metabolism rat urine Animal Carbon Isotopes Chickens Erythrocytes Female Glycine Heme Hemolysis Male Porphyrins Rats 1. 1. The role of 5- and 6-COOH porphyrinogens III in haem biosynthesis was studied in vitro. 2. 2. When haemolysates of normal chicken erythrocytes were incubated in the presence of 5- or 6-COOH porphyrinogens III, a marked isotopic dilution was observed served on the [2-12C]glycine incorporation into haem. This effect was similar and even greater than that produced by uroporphyrinogen III and coproporphyrinogen III. To confirm these results 14C-labelled porphyrinogens of 5- and 6-COOH were incubated with chicken erythrocyte haemolysates. Labelling of the haem took place in a way similar to that when [14C]uroporphyrinogen III was used. 3. 3. These results are consistent with the idea that 5-COOH porphyrinogen III and 6-COOH porphyrinogen III are normal intermediates in haem biosynthesis with a stepwise decarboxylation process of uroporphyrinogen III to coproporphyrinogen III according to the following scheme: Uroporphyrinogen III → 7-COOH porphyrinogen III → 6-COOH porphyrinogen III → 5-COOH porphyrinogen III → coproporphyrinogen III. © 1968. Fil:San Martín De Viale, L.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Grinstein, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03044165_v158_n1_p79_SanMartinDeViale |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
carbon glycine heme porphyrin animal article biosynthesis chicken enzymology erythrocyte female hemolysis male metabolism rat urine Animal Carbon Isotopes Chickens Erythrocytes Female Glycine Heme Hemolysis Male Porphyrins Rats |
| spellingShingle |
carbon glycine heme porphyrin animal article biosynthesis chicken enzymology erythrocyte female hemolysis male metabolism rat urine Animal Carbon Isotopes Chickens Erythrocytes Female Glycine Heme Hemolysis Male Porphyrins Rats San Martín De Viale, L.C. Grinstein, M. Grinstein, M. Porphyrin biosynthesis. IV. 5- and 6-COOH porphyrinogens (type III) as normal intermediates in haem biosynthesis |
| topic_facet |
carbon glycine heme porphyrin animal article biosynthesis chicken enzymology erythrocyte female hemolysis male metabolism rat urine Animal Carbon Isotopes Chickens Erythrocytes Female Glycine Heme Hemolysis Male Porphyrins Rats |
| description |
1. 1. The role of 5- and 6-COOH porphyrinogens III in haem biosynthesis was studied in vitro. 2. 2. When haemolysates of normal chicken erythrocytes were incubated in the presence of 5- or 6-COOH porphyrinogens III, a marked isotopic dilution was observed served on the [2-12C]glycine incorporation into haem. This effect was similar and even greater than that produced by uroporphyrinogen III and coproporphyrinogen III. To confirm these results 14C-labelled porphyrinogens of 5- and 6-COOH were incubated with chicken erythrocyte haemolysates. Labelling of the haem took place in a way similar to that when [14C]uroporphyrinogen III was used. 3. 3. These results are consistent with the idea that 5-COOH porphyrinogen III and 6-COOH porphyrinogen III are normal intermediates in haem biosynthesis with a stepwise decarboxylation process of uroporphyrinogen III to coproporphyrinogen III according to the following scheme: Uroporphyrinogen III → 7-COOH porphyrinogen III → 6-COOH porphyrinogen III → 5-COOH porphyrinogen III → coproporphyrinogen III. © 1968. |
| format |
JOUR |
| author |
San Martín De Viale, L.C. Grinstein, M. Grinstein, M. |
| author_facet |
San Martín De Viale, L.C. Grinstein, M. Grinstein, M. |
| author_sort |
San Martín De Viale, L.C. |
| title |
Porphyrin biosynthesis. IV. 5- and 6-COOH porphyrinogens (type III) as normal intermediates in haem biosynthesis |
| title_short |
Porphyrin biosynthesis. IV. 5- and 6-COOH porphyrinogens (type III) as normal intermediates in haem biosynthesis |
| title_full |
Porphyrin biosynthesis. IV. 5- and 6-COOH porphyrinogens (type III) as normal intermediates in haem biosynthesis |
| title_fullStr |
Porphyrin biosynthesis. IV. 5- and 6-COOH porphyrinogens (type III) as normal intermediates in haem biosynthesis |
| title_full_unstemmed |
Porphyrin biosynthesis. IV. 5- and 6-COOH porphyrinogens (type III) as normal intermediates in haem biosynthesis |
| title_sort |
porphyrin biosynthesis. iv. 5- and 6-cooh porphyrinogens (type iii) as normal intermediates in haem biosynthesis |
| url |
http://hdl.handle.net/20.500.12110/paper_03044165_v158_n1_p79_SanMartinDeViale |
| work_keys_str_mv |
AT sanmartindevialelc porphyrinbiosynthesisiv5and6coohporphyrinogenstypeiiiasnormalintermediatesinhaembiosynthesis AT grinsteinm porphyrinbiosynthesisiv5and6coohporphyrinogenstypeiiiasnormalintermediatesinhaembiosynthesis AT grinsteinm porphyrinbiosynthesisiv5and6coohporphyrinogenstypeiiiasnormalintermediatesinhaembiosynthesis |
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1807319516384854016 |