Mechanical coupling of microtubule-dependent motor teams during peroxisome transport in Drosophila S2 cells
Background Intracellular transport requires molecular motors that step along cytoskeletal filaments actively dragging cargoes through the crowded cytoplasm. Here, we explore the interplay of the opposed polarity motors kinesin-1 and cytoplasmic dynein during peroxisome transport along microtubules i...
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todo:paper_03044165_v1861_n12_p3178_DeRossi2023-10-03T15:20:46Z Mechanical coupling of microtubule-dependent motor teams during peroxisome transport in Drosophila S2 cells De Rossi, M.C. Wetzler, D.E. Benseñor, L. De Rossi, M.E. Sued, M. Rodríguez, D. Gelfand, V. Bruno, L. Levi, V. Drosophila S2 cells Intracellular transport Molecular motors Single particle tracking dynein adenosine triphosphatase kinesin 1 methyl beta cyclodextrin beta cyclodextrin derivative methyl-beta-cyclodextrin Article cell membrane fluidity cell motion cell tracking cell transport cellular distribution chemical procedures chemical reaction controlled study Drosophila melanogaster measurement accuracy mechanical coupling microtubule millisecond time resolution nonhuman peroxisome priority journal simulation single particle tracking velocity animal cell culture Drosophila membrane fluidity metabolism microtubule peroxisome physiology transport at the cellular level Animals beta-Cyclodextrins Biological Transport Cells, Cultured Drosophila Membrane Fluidity Microtubules Peroxisomes Background Intracellular transport requires molecular motors that step along cytoskeletal filaments actively dragging cargoes through the crowded cytoplasm. Here, we explore the interplay of the opposed polarity motors kinesin-1 and cytoplasmic dynein during peroxisome transport along microtubules in Drosophila S2 cells. Methods We used single particle tracking with nanometer accuracy and millisecond time resolution to extract quantitative information on the bidirectional motion of organelles. The transport performance was studied in cells expressing a slow chimeric plus-end directed motor or the kinesin heavy chain. We also analyzed the influence of peroxisomes membrane fluidity in methyl-β-ciclodextrin treated cells. The experimental data was also confronted with numerical simulations of two well-established tug of war scenarios. Results and conclusions The velocity distributions of retrograde and anterograde peroxisomes showed a multimodal pattern suggesting that multiple motor teams drive transport in either direction. The chimeric motors interfered with the performance of anterograde transport and also reduced the speed of the slowest retrograde team. In addition, increasing the fluidity of peroxisomes membrane decreased the speed of the slowest anterograde and retrograde teams. General significance Our results support the existence of a crosstalk between opposed-polarity motor teams. Moreover, the slowest teams seem to mechanically communicate with each other through the membrane to trigger transport. © 2017 Elsevier B.V. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03044165_v1861_n12_p3178_DeRossi |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Drosophila S2 cells Intracellular transport Molecular motors Single particle tracking dynein adenosine triphosphatase kinesin 1 methyl beta cyclodextrin beta cyclodextrin derivative methyl-beta-cyclodextrin Article cell membrane fluidity cell motion cell tracking cell transport cellular distribution chemical procedures chemical reaction controlled study Drosophila melanogaster measurement accuracy mechanical coupling microtubule millisecond time resolution nonhuman peroxisome priority journal simulation single particle tracking velocity animal cell culture Drosophila membrane fluidity metabolism microtubule peroxisome physiology transport at the cellular level Animals beta-Cyclodextrins Biological Transport Cells, Cultured Drosophila Membrane Fluidity Microtubules Peroxisomes |
| spellingShingle |
Drosophila S2 cells Intracellular transport Molecular motors Single particle tracking dynein adenosine triphosphatase kinesin 1 methyl beta cyclodextrin beta cyclodextrin derivative methyl-beta-cyclodextrin Article cell membrane fluidity cell motion cell tracking cell transport cellular distribution chemical procedures chemical reaction controlled study Drosophila melanogaster measurement accuracy mechanical coupling microtubule millisecond time resolution nonhuman peroxisome priority journal simulation single particle tracking velocity animal cell culture Drosophila membrane fluidity metabolism microtubule peroxisome physiology transport at the cellular level Animals beta-Cyclodextrins Biological Transport Cells, Cultured Drosophila Membrane Fluidity Microtubules Peroxisomes De Rossi, M.C. Wetzler, D.E. Benseñor, L. De Rossi, M.E. Sued, M. Rodríguez, D. Gelfand, V. Bruno, L. Levi, V. Mechanical coupling of microtubule-dependent motor teams during peroxisome transport in Drosophila S2 cells |
| topic_facet |
Drosophila S2 cells Intracellular transport Molecular motors Single particle tracking dynein adenosine triphosphatase kinesin 1 methyl beta cyclodextrin beta cyclodextrin derivative methyl-beta-cyclodextrin Article cell membrane fluidity cell motion cell tracking cell transport cellular distribution chemical procedures chemical reaction controlled study Drosophila melanogaster measurement accuracy mechanical coupling microtubule millisecond time resolution nonhuman peroxisome priority journal simulation single particle tracking velocity animal cell culture Drosophila membrane fluidity metabolism microtubule peroxisome physiology transport at the cellular level Animals beta-Cyclodextrins Biological Transport Cells, Cultured Drosophila Membrane Fluidity Microtubules Peroxisomes |
| description |
Background Intracellular transport requires molecular motors that step along cytoskeletal filaments actively dragging cargoes through the crowded cytoplasm. Here, we explore the interplay of the opposed polarity motors kinesin-1 and cytoplasmic dynein during peroxisome transport along microtubules in Drosophila S2 cells. Methods We used single particle tracking with nanometer accuracy and millisecond time resolution to extract quantitative information on the bidirectional motion of organelles. The transport performance was studied in cells expressing a slow chimeric plus-end directed motor or the kinesin heavy chain. We also analyzed the influence of peroxisomes membrane fluidity in methyl-β-ciclodextrin treated cells. The experimental data was also confronted with numerical simulations of two well-established tug of war scenarios. Results and conclusions The velocity distributions of retrograde and anterograde peroxisomes showed a multimodal pattern suggesting that multiple motor teams drive transport in either direction. The chimeric motors interfered with the performance of anterograde transport and also reduced the speed of the slowest retrograde team. In addition, increasing the fluidity of peroxisomes membrane decreased the speed of the slowest anterograde and retrograde teams. General significance Our results support the existence of a crosstalk between opposed-polarity motor teams. Moreover, the slowest teams seem to mechanically communicate with each other through the membrane to trigger transport. © 2017 Elsevier B.V. |
| format |
JOUR |
| author |
De Rossi, M.C. Wetzler, D.E. Benseñor, L. De Rossi, M.E. Sued, M. Rodríguez, D. Gelfand, V. Bruno, L. Levi, V. |
| author_facet |
De Rossi, M.C. Wetzler, D.E. Benseñor, L. De Rossi, M.E. Sued, M. Rodríguez, D. Gelfand, V. Bruno, L. Levi, V. |
| author_sort |
De Rossi, M.C. |
| title |
Mechanical coupling of microtubule-dependent motor teams during peroxisome transport in Drosophila S2 cells |
| title_short |
Mechanical coupling of microtubule-dependent motor teams during peroxisome transport in Drosophila S2 cells |
| title_full |
Mechanical coupling of microtubule-dependent motor teams during peroxisome transport in Drosophila S2 cells |
| title_fullStr |
Mechanical coupling of microtubule-dependent motor teams during peroxisome transport in Drosophila S2 cells |
| title_full_unstemmed |
Mechanical coupling of microtubule-dependent motor teams during peroxisome transport in Drosophila S2 cells |
| title_sort |
mechanical coupling of microtubule-dependent motor teams during peroxisome transport in drosophila s2 cells |
| url |
http://hdl.handle.net/20.500.12110/paper_03044165_v1861_n12_p3178_DeRossi |
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1807324655509307392 |