Expression of the human RNA-binding protein HuR in Trypanosoma brucei increases the abundance of mRNAs containing AU-rich regulatory elements
The salivarian trypanosome Trypanosoma brucei infects mammals and is transmitted by tsetse flies. The mammalian 'bloodstream form' trypanosome has a variant surface glycoprotein coat and relies on glycolysis while the procyclic form from tsetse flies has EP protein on the surface and has a...
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todo:paper_03051048_v30_n20_p4414_Quijada2023-10-03T15:21:30Z Expression of the human RNA-binding protein HuR in Trypanosoma brucei increases the abundance of mRNAs containing AU-rich regulatory elements Quijada, L. Guerra-Giraldez, C. Drozdz, M. Hartmann, C. Irmer, H. Ben-Dov, C. Cristodero, M. Ding, M. Clayton, C. adenine messenger RNA phosphoglycerate kinase protozoal protein pyruvic acid RNA binding protein uracil variant surface glycoprotein article controlled study disease transmission eukaryote evolution glycolysis growth inhibition mitochondrion nonhuman priority journal protein expression protein RNA binding protein stability RNA stability RNA structure RNA synthesis Trypanosoma Trypanosoma brucei tsetse fly 3' Untranslated Regions Adenine Animals Antigens, Surface Base Sequence Gene Expression Regulation, Developmental Humans Molecular Sequence Data Phosphoglycerate Kinase Protozoan Proteins Regulatory Sequences, Nucleic Acid RNA Stability RNA, Messenger RNA, Protozoan RNA-Binding Proteins Trypanosoma brucei brucei Uracil Eukaryota Glossina (proper) Mammalia Protozoa Trypanosoma Trypanosoma brucei The salivarian trypanosome Trypanosoma brucei infects mammals and is transmitted by tsetse flies. The mammalian 'bloodstream form' trypanosome has a variant surface glycoprotein coat and relies on glycolysis while the procyclic form from tsetse flies has EP protein on the surface and has a more developed mitochondrion. We show here that the mRNA for the procyclic-specific cytosolic phosphoglycerate kinase PGKB, like that for EP proteins, contains a regulatory AU-rich element (ARE) that destabilises the mRNA in bloodstream forms. The human HuR protein binds to, and stabilises, mammalian mRNAs containing AREs. Expression of HuR in bloodstream-form trypanosomes resulted in growth arrest and in stabilisation of the EP, PGKB and pyruvate, phosphate dikinase mRNAs, while three bloodstream-specific mRNAs were reduced in abundance. The synthesis and abundance of unregulated mRNAs and proteins were unaffected. Our results suggest that regulation of mRNA stability by AREs arose early in eukaryotic evolution. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03051048_v30_n20_p4414_Quijada |
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Universidad de Buenos Aires |
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I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
adenine messenger RNA phosphoglycerate kinase protozoal protein pyruvic acid RNA binding protein uracil variant surface glycoprotein article controlled study disease transmission eukaryote evolution glycolysis growth inhibition mitochondrion nonhuman priority journal protein expression protein RNA binding protein stability RNA stability RNA structure RNA synthesis Trypanosoma Trypanosoma brucei tsetse fly 3' Untranslated Regions Adenine Animals Antigens, Surface Base Sequence Gene Expression Regulation, Developmental Humans Molecular Sequence Data Phosphoglycerate Kinase Protozoan Proteins Regulatory Sequences, Nucleic Acid RNA Stability RNA, Messenger RNA, Protozoan RNA-Binding Proteins Trypanosoma brucei brucei Uracil Eukaryota Glossina (proper) Mammalia Protozoa Trypanosoma Trypanosoma brucei |
| spellingShingle |
adenine messenger RNA phosphoglycerate kinase protozoal protein pyruvic acid RNA binding protein uracil variant surface glycoprotein article controlled study disease transmission eukaryote evolution glycolysis growth inhibition mitochondrion nonhuman priority journal protein expression protein RNA binding protein stability RNA stability RNA structure RNA synthesis Trypanosoma Trypanosoma brucei tsetse fly 3' Untranslated Regions Adenine Animals Antigens, Surface Base Sequence Gene Expression Regulation, Developmental Humans Molecular Sequence Data Phosphoglycerate Kinase Protozoan Proteins Regulatory Sequences, Nucleic Acid RNA Stability RNA, Messenger RNA, Protozoan RNA-Binding Proteins Trypanosoma brucei brucei Uracil Eukaryota Glossina (proper) Mammalia Protozoa Trypanosoma Trypanosoma brucei Quijada, L. Guerra-Giraldez, C. Drozdz, M. Hartmann, C. Irmer, H. Ben-Dov, C. Cristodero, M. Ding, M. Clayton, C. Expression of the human RNA-binding protein HuR in Trypanosoma brucei increases the abundance of mRNAs containing AU-rich regulatory elements |
| topic_facet |
adenine messenger RNA phosphoglycerate kinase protozoal protein pyruvic acid RNA binding protein uracil variant surface glycoprotein article controlled study disease transmission eukaryote evolution glycolysis growth inhibition mitochondrion nonhuman priority journal protein expression protein RNA binding protein stability RNA stability RNA structure RNA synthesis Trypanosoma Trypanosoma brucei tsetse fly 3' Untranslated Regions Adenine Animals Antigens, Surface Base Sequence Gene Expression Regulation, Developmental Humans Molecular Sequence Data Phosphoglycerate Kinase Protozoan Proteins Regulatory Sequences, Nucleic Acid RNA Stability RNA, Messenger RNA, Protozoan RNA-Binding Proteins Trypanosoma brucei brucei Uracil Eukaryota Glossina (proper) Mammalia Protozoa Trypanosoma Trypanosoma brucei |
| description |
The salivarian trypanosome Trypanosoma brucei infects mammals and is transmitted by tsetse flies. The mammalian 'bloodstream form' trypanosome has a variant surface glycoprotein coat and relies on glycolysis while the procyclic form from tsetse flies has EP protein on the surface and has a more developed mitochondrion. We show here that the mRNA for the procyclic-specific cytosolic phosphoglycerate kinase PGKB, like that for EP proteins, contains a regulatory AU-rich element (ARE) that destabilises the mRNA in bloodstream forms. The human HuR protein binds to, and stabilises, mammalian mRNAs containing AREs. Expression of HuR in bloodstream-form trypanosomes resulted in growth arrest and in stabilisation of the EP, PGKB and pyruvate, phosphate dikinase mRNAs, while three bloodstream-specific mRNAs were reduced in abundance. The synthesis and abundance of unregulated mRNAs and proteins were unaffected. Our results suggest that regulation of mRNA stability by AREs arose early in eukaryotic evolution. |
| format |
JOUR |
| author |
Quijada, L. Guerra-Giraldez, C. Drozdz, M. Hartmann, C. Irmer, H. Ben-Dov, C. Cristodero, M. Ding, M. Clayton, C. |
| author_facet |
Quijada, L. Guerra-Giraldez, C. Drozdz, M. Hartmann, C. Irmer, H. Ben-Dov, C. Cristodero, M. Ding, M. Clayton, C. |
| author_sort |
Quijada, L. |
| title |
Expression of the human RNA-binding protein HuR in Trypanosoma brucei increases the abundance of mRNAs containing AU-rich regulatory elements |
| title_short |
Expression of the human RNA-binding protein HuR in Trypanosoma brucei increases the abundance of mRNAs containing AU-rich regulatory elements |
| title_full |
Expression of the human RNA-binding protein HuR in Trypanosoma brucei increases the abundance of mRNAs containing AU-rich regulatory elements |
| title_fullStr |
Expression of the human RNA-binding protein HuR in Trypanosoma brucei increases the abundance of mRNAs containing AU-rich regulatory elements |
| title_full_unstemmed |
Expression of the human RNA-binding protein HuR in Trypanosoma brucei increases the abundance of mRNAs containing AU-rich regulatory elements |
| title_sort |
expression of the human rna-binding protein hur in trypanosoma brucei increases the abundance of mrnas containing au-rich regulatory elements |
| url |
http://hdl.handle.net/20.500.12110/paper_03051048_v30_n20_p4414_Quijada |
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1807316421697339392 |