Expression of the human RNA-binding protein HuR in Trypanosoma brucei increases the abundance of mRNAs containing AU-rich regulatory elements

The salivarian trypanosome Trypanosoma brucei infects mammals and is transmitted by tsetse flies. The mammalian 'bloodstream form' trypanosome has a variant surface glycoprotein coat and relies on glycolysis while the procyclic form from tsetse flies has EP protein on the surface and has a...

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Detalles Bibliográficos
Autores principales: Quijada, L., Guerra-Giraldez, C., Drozdz, M., Hartmann, C., Irmer, H., Ben-Dov, C., Cristodero, M., Ding, M., Clayton, C.
Formato: JOUR
Materias:
adenine
messenger RNA
phosphoglycerate kinase
protozoal protein
pyruvic acid
RNA binding protein
uracil
variant surface glycoprotein
article
controlled study
disease transmission
eukaryote
evolution
glycolysis
growth inhibition
mitochondrion
nonhuman
priority journal
protein expression
protein RNA binding
protein stability
RNA stability
RNA structure
RNA synthesis
Trypanosoma
Trypanosoma brucei
tsetse fly
3' Untranslated Regions
Adenine
Animals
Antigens, Surface
Base Sequence
Gene Expression Regulation, Developmental
Humans
Molecular Sequence Data
Phosphoglycerate Kinase
Protozoan Proteins
Regulatory Sequences, Nucleic Acid
RNA Stability
RNA, Messenger
RNA, Protozoan
RNA-Binding Proteins
Trypanosoma brucei brucei
Uracil
Eukaryota
Glossina (proper)
Mammalia
Protozoa
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03051048_v30_n20_p4414_Quijada
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:The salivarian trypanosome Trypanosoma brucei infects mammals and is transmitted by tsetse flies. The mammalian 'bloodstream form' trypanosome has a variant surface glycoprotein coat and relies on glycolysis while the procyclic form from tsetse flies has EP protein on the surface and has a more developed mitochondrion. We show here that the mRNA for the procyclic-specific cytosolic phosphoglycerate kinase PGKB, like that for EP proteins, contains a regulatory AU-rich element (ARE) that destabilises the mRNA in bloodstream forms. The human HuR protein binds to, and stabilises, mammalian mRNAs containing AREs. Expression of HuR in bloodstream-form trypanosomes resulted in growth arrest and in stabilisation of the EP, PGKB and pyruvate, phosphate dikinase mRNAs, while three bloodstream-specific mRNAs were reduced in abundance. The synthesis and abundance of unregulated mRNAs and proteins were unaffected. Our results suggest that regulation of mRNA stability by AREs arose early in eukaryotic evolution.

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