Amygdaloid pERK1/2 in corticotropin-releasing hormone overexpressing mice under basal and acute stress conditions

Corticotropin-releasing hormone (CRH) coordinates neuroendocrine and behavioral adaptations to stress. Acute CRH administration in vivo activates extracellular signal-regulated kinase 1/2 (ERK1/2) in limbic brain areas, acting through the CRH receptor type 1 (CRH-R1). In the present study, we used C...

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Autores principales:	Silberstein, S., Vogl, A.M., Refojo, D., Senin, S.A., Wurst, W., Holsboer, F., Deussing, J.M., Arzt, E.
Formato:	JOUR
Materias:	CRF CRH CRH receptor ERK HPA axis mitogen activated protein kinase 1 mitogen activated protein kinase 3 acute stress amygdaloid nucleus animal experiment article brain region controlled study immunohistochemistry in situ hybridization mouse nonhuman priority journal protein expression Amphibian Proteins Amygdala Animals Autoradiography Corticosterone Corticotropin-Releasing Hormone Disease Models, Animal Gene Expression Regulation, Enzymologic Iodine Isotopes Male Mice Mice, Transgenic Mitogen-Activated Protein Kinase 3 Peptide Hormones Protein Binding Radioimmunoassay Receptors, Corticotropin-Releasing Hormone Restraint, Physical Stress, Psychological Time Factors
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_03064522_v159_n2_p610_Silberstein
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_03064522_v159_n2_p610_Silberstein2023-10-03T15:22:23Z Amygdaloid pERK1/2 in corticotropin-releasing hormone overexpressing mice under basal and acute stress conditions Silberstein, S. Vogl, A.M. Refojo, D. Senin, S.A. Wurst, W. Holsboer, F. Deussing, J.M. Arzt, E. CRF CRH CRH receptor ERK HPA axis mitogen activated protein kinase 1 mitogen activated protein kinase 3 acute stress amygdaloid nucleus animal experiment article brain region controlled study immunohistochemistry in situ hybridization mouse nonhuman priority journal protein expression Amphibian Proteins Amygdala Animals Autoradiography Corticosterone Corticotropin-Releasing Hormone Disease Models, Animal Gene Expression Regulation, Enzymologic Iodine Isotopes Male Mice Mice, Transgenic Mitogen-Activated Protein Kinase 3 Peptide Hormones Protein Binding Radioimmunoassay Receptors, Corticotropin-Releasing Hormone Restraint, Physical Stress, Psychological Time Factors Corticotropin-releasing hormone (CRH) coordinates neuroendocrine and behavioral adaptations to stress. Acute CRH administration in vivo activates extracellular signal-regulated kinase 1/2 (ERK1/2) in limbic brain areas, acting through the CRH receptor type 1 (CRH-R1). In the present study, we used CRH-COE-Cam mice that overexpress CRH in limbic-restricted areas, to analyze the effect of chronic CRH overexpression on ERK1/2 activation. By immunohistochemistry and confocal microscopy analysis we found that pERK1/2 levels in the basolateral amygdala (BLA) were similar in control and CRH overexpressing mice under basal conditions. Acute stress caused comparably increased levels of corticosterone in both control (CRH-COEcon-Cam) and CRH overexpressing (CRH-COEhom-Cam) animals. CRH-COEhom-Cam mice after stress showed reduced pERK1/2 immunoreactivity in the BLA compared to CRH-COEhom-Cam animals under basal conditions. Radioligand binding and in situ hybridization revealed higher density of CRH-R1 in the amygdala of CRH-COEhom mice under basal conditions compared to control littermates. A significant reduction of the receptor levels was observed in this area after acute stress, suggesting that stress may trigger CRH-R1 internalization/downregulation in these CRH overexpressing mice. Chronic CRH overexpression leads to reduced ERK1/2 activation in response to acute stress in the BLA. © 2009 IBRO. Fil:Silberstein, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Refojo, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03064522_v159_n2_p610_Silberstein
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	CRF CRH CRH receptor ERK HPA axis mitogen activated protein kinase 1 mitogen activated protein kinase 3 acute stress amygdaloid nucleus animal experiment article brain region controlled study immunohistochemistry in situ hybridization mouse nonhuman priority journal protein expression Amphibian Proteins Amygdala Animals Autoradiography Corticosterone Corticotropin-Releasing Hormone Disease Models, Animal Gene Expression Regulation, Enzymologic Iodine Isotopes Male Mice Mice, Transgenic Mitogen-Activated Protein Kinase 3 Peptide Hormones Protein Binding Radioimmunoassay Receptors, Corticotropin-Releasing Hormone Restraint, Physical Stress, Psychological Time Factors
spellingShingle	CRF CRH CRH receptor ERK HPA axis mitogen activated protein kinase 1 mitogen activated protein kinase 3 acute stress amygdaloid nucleus animal experiment article brain region controlled study immunohistochemistry in situ hybridization mouse nonhuman priority journal protein expression Amphibian Proteins Amygdala Animals Autoradiography Corticosterone Corticotropin-Releasing Hormone Disease Models, Animal Gene Expression Regulation, Enzymologic Iodine Isotopes Male Mice Mice, Transgenic Mitogen-Activated Protein Kinase 3 Peptide Hormones Protein Binding Radioimmunoassay Receptors, Corticotropin-Releasing Hormone Restraint, Physical Stress, Psychological Time Factors Silberstein, S. Vogl, A.M. Refojo, D. Senin, S.A. Wurst, W. Holsboer, F. Deussing, J.M. Arzt, E. Amygdaloid pERK1/2 in corticotropin-releasing hormone overexpressing mice under basal and acute stress conditions
topic_facet	CRF CRH CRH receptor ERK HPA axis mitogen activated protein kinase 1 mitogen activated protein kinase 3 acute stress amygdaloid nucleus animal experiment article brain region controlled study immunohistochemistry in situ hybridization mouse nonhuman priority journal protein expression Amphibian Proteins Amygdala Animals Autoradiography Corticosterone Corticotropin-Releasing Hormone Disease Models, Animal Gene Expression Regulation, Enzymologic Iodine Isotopes Male Mice Mice, Transgenic Mitogen-Activated Protein Kinase 3 Peptide Hormones Protein Binding Radioimmunoassay Receptors, Corticotropin-Releasing Hormone Restraint, Physical Stress, Psychological Time Factors
description	Corticotropin-releasing hormone (CRH) coordinates neuroendocrine and behavioral adaptations to stress. Acute CRH administration in vivo activates extracellular signal-regulated kinase 1/2 (ERK1/2) in limbic brain areas, acting through the CRH receptor type 1 (CRH-R1). In the present study, we used CRH-COE-Cam mice that overexpress CRH in limbic-restricted areas, to analyze the effect of chronic CRH overexpression on ERK1/2 activation. By immunohistochemistry and confocal microscopy analysis we found that pERK1/2 levels in the basolateral amygdala (BLA) were similar in control and CRH overexpressing mice under basal conditions. Acute stress caused comparably increased levels of corticosterone in both control (CRH-COEcon-Cam) and CRH overexpressing (CRH-COEhom-Cam) animals. CRH-COEhom-Cam mice after stress showed reduced pERK1/2 immunoreactivity in the BLA compared to CRH-COEhom-Cam animals under basal conditions. Radioligand binding and in situ hybridization revealed higher density of CRH-R1 in the amygdala of CRH-COEhom mice under basal conditions compared to control littermates. A significant reduction of the receptor levels was observed in this area after acute stress, suggesting that stress may trigger CRH-R1 internalization/downregulation in these CRH overexpressing mice. Chronic CRH overexpression leads to reduced ERK1/2 activation in response to acute stress in the BLA. © 2009 IBRO.
format	JOUR
author	Silberstein, S. Vogl, A.M. Refojo, D. Senin, S.A. Wurst, W. Holsboer, F. Deussing, J.M. Arzt, E.
author_facet	Silberstein, S. Vogl, A.M. Refojo, D. Senin, S.A. Wurst, W. Holsboer, F. Deussing, J.M. Arzt, E.
author_sort	Silberstein, S.
title	Amygdaloid pERK1/2 in corticotropin-releasing hormone overexpressing mice under basal and acute stress conditions
title_short	Amygdaloid pERK1/2 in corticotropin-releasing hormone overexpressing mice under basal and acute stress conditions
title_full	Amygdaloid pERK1/2 in corticotropin-releasing hormone overexpressing mice under basal and acute stress conditions
title_fullStr	Amygdaloid pERK1/2 in corticotropin-releasing hormone overexpressing mice under basal and acute stress conditions
title_full_unstemmed	Amygdaloid pERK1/2 in corticotropin-releasing hormone overexpressing mice under basal and acute stress conditions
title_sort	amygdaloid perk1/2 in corticotropin-releasing hormone overexpressing mice under basal and acute stress conditions
url	http://hdl.handle.net/20.500.12110/paper_03064522_v159_n2_p610_Silberstein
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Amygdaloid pERK1/2 in corticotropin-releasing hormone overexpressing mice under basal and acute stress conditions

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