Isolation of new serovars of Salmonella in streams of water

The present work is the final stage of a research to determine the causes and degree of pollution of the Zaiman creek waters, which flow through the Capital District of Misiones, Argentina. The research was focused on the presence of Salmonella serovars in samples collected from 2 stream sites equid...

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Autores principales: Benassi, F.O., Martínez Vázquez, F., Eiguer, T., Bendersky, S., Martos, M.A.
Formato: JOUR
Materias:
fresh water
Argentina
article
classification
isolation and purification
microbiology
Salmonella
water pollution
Fresh Water
Water Microbiology
Water Pollution
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03257541_v17_n3_p149_Benassi
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_03257541_v17_n3_p149_Benassi
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spelling todo:paper_03257541_v17_n3_p149_Benassi2023-10-03T15:23:53Z Isolation of new serovars of Salmonella in streams of water Benassi, F.O. Martínez Vázquez, F. Eiguer, T. Bendersky, S. Martos, M.A. fresh water Argentina article classification isolation and purification microbiology Salmonella water pollution Argentina Fresh Water Salmonella Water Microbiology Water Pollution The present work is the final stage of a research to determine the causes and degree of pollution of the Zaiman creek waters, which flow through the Capital District of Misiones, Argentina. The research was focused on the presence of Salmonella serovars in samples collected from 2 stream sites equidistant from a cold storage plant and slaughterhouse, one downstream, and the other before the source of pollution. Elevated temperature technique for the isolation of Salmonella was applied. The methodology consisted in applying an enrichment procedure for waterborne pathogens, performed by incubating after a convenient exposure period, gauze swabs in Dulcitol Selenite and Tetrationate (with the addition of 0,001% brilliant green) broths. Salmonella organisms were recovered by streaking duplicate plates of E.M.B Agar (Eosin Methylene Blue), B.P.L.S Agar (Brilliant Green Agar) and Bismuth Sulphite Agar. Best results were obtained when Bulcitol Selenite broth and Bismuth Sulphite Agar were used. (Tables 1, 2, 3). Suspected colonies were classified by 10 biochemical reactions, and further verified by serological identification. So far, 716 suspected colonies have been studied. Salmonella was isolated from 76% of the samples and 22 serovars were identified (Table 3). Isolating frequency of serovars during 1981-1984 is shown (Table 4). S. saphra prevailed among the studied samples, while both S. anatum and S. newport ranked second.(ABSTRACT TRUNCATED AT 250 WORDS) Fil:Eiguer, T. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03257541_v17_n3_p149_Benassi
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic fresh water
Argentina
article
classification
isolation and purification
microbiology
Salmonella
water pollution
Argentina
Fresh Water
Salmonella
Water Microbiology
Water Pollution
spellingShingle fresh water
Argentina
article
classification
isolation and purification
microbiology
Salmonella
water pollution
Argentina
Fresh Water
Salmonella
Water Microbiology
Water Pollution
Benassi, F.O.
Martínez Vázquez, F.
Eiguer, T.
Bendersky, S.
Martos, M.A.
Isolation of new serovars of Salmonella in streams of water
topic_facet fresh water
Argentina
article
classification
isolation and purification
microbiology
Salmonella
water pollution
Argentina
Fresh Water
Salmonella
Water Microbiology
Water Pollution
description The present work is the final stage of a research to determine the causes and degree of pollution of the Zaiman creek waters, which flow through the Capital District of Misiones, Argentina. The research was focused on the presence of Salmonella serovars in samples collected from 2 stream sites equidistant from a cold storage plant and slaughterhouse, one downstream, and the other before the source of pollution. Elevated temperature technique for the isolation of Salmonella was applied. The methodology consisted in applying an enrichment procedure for waterborne pathogens, performed by incubating after a convenient exposure period, gauze swabs in Dulcitol Selenite and Tetrationate (with the addition of 0,001% brilliant green) broths. Salmonella organisms were recovered by streaking duplicate plates of E.M.B Agar (Eosin Methylene Blue), B.P.L.S Agar (Brilliant Green Agar) and Bismuth Sulphite Agar. Best results were obtained when Bulcitol Selenite broth and Bismuth Sulphite Agar were used. (Tables 1, 2, 3). Suspected colonies were classified by 10 biochemical reactions, and further verified by serological identification. So far, 716 suspected colonies have been studied. Salmonella was isolated from 76% of the samples and 22 serovars were identified (Table 3). Isolating frequency of serovars during 1981-1984 is shown (Table 4). S. saphra prevailed among the studied samples, while both S. anatum and S. newport ranked second.(ABSTRACT TRUNCATED AT 250 WORDS)
format JOUR
author Benassi, F.O.
Martínez Vázquez, F.
Eiguer, T.
Bendersky, S.
Martos, M.A.
author_facet Benassi, F.O.
Martínez Vázquez, F.
Eiguer, T.
Bendersky, S.
Martos, M.A.
author_sort Benassi, F.O.
title Isolation of new serovars of Salmonella in streams of water
title_short Isolation of new serovars of Salmonella in streams of water
title_full Isolation of new serovars of Salmonella in streams of water
title_fullStr Isolation of new serovars of Salmonella in streams of water
title_full_unstemmed Isolation of new serovars of Salmonella in streams of water
title_sort isolation of new serovars of salmonella in streams of water
url http://hdl.handle.net/20.500.12110/paper_03257541_v17_n3_p149_Benassi
work_keys_str_mv AT benassifo isolationofnewserovarsofsalmonellainstreamsofwater
AT martinezvazquezf isolationofnewserovarsofsalmonellainstreamsofwater
AT eiguert isolationofnewserovarsofsalmonellainstreamsofwater
AT benderskys isolationofnewserovarsofsalmonellainstreamsofwater
AT martosma isolationofnewserovarsofsalmonellainstreamsofwater
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