Presence of Acanthamoeba spp. in watering trough in La Pampa province, Argentina

Free-living Amoebae of Acanthamoeba genus include non-pathogenic and pathogenic strains that are currently classified in 18 different genotypes, T1-T18. In this study, a survey was carried out to evaluate the presence of Acanthamoeba strains inwatering trough sample in La Pampa province, Argentina....

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Detalles Bibliográficos
Autores principales:	Rojas, M.D.C., Rodríguez Fermepín, M., Gracia Martínez, F., Costamagna, S.R.
Formato:	INPR
Materias:	Acanthamoeba Genotyping La Pampa PCR Watering trough Argentina clinical article genotype genus livestock nonhuman polymerase chain reaction veterinary pathology
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_03257541_v_n_p_Rojas
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_03257541_v_n_p_Rojas
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spelling	todo:paper_03257541_v_n_p_Rojas2023-10-03T15:24:25Z Presence of Acanthamoeba spp. in watering trough in La Pampa province, Argentina Rojas, M.D.C. Rodríguez Fermepín, M. Gracia Martínez, F. Costamagna, S.R. Acanthamoeba Genotyping La Pampa PCR Watering trough Acanthamoeba Argentina clinical article genotype genus livestock nonhuman polymerase chain reaction veterinary pathology Free-living Amoebae of Acanthamoeba genus include non-pathogenic and pathogenic strains that are currently classified in 18 different genotypes, T1-T18. In this study, a survey was carried out to evaluate the presence of Acanthamoeba strains inwatering trough sample in La Pampa province, Argentina. Sample were inoculated onto non-nutrient agar plates and were checked for the presence of Acanthamoeba. Polymerase chain reaction was performed with genus-specific primers JDP1/JDP2, followed by direct sequencing of the polymerase chain reaction product for molecular identification. Sequencing results revealed the presence of T4, T5 and T15 genotypes within the studied samples. Sequencing revealed presence of T4, T5 and T15 in the samples studied genotypes, the most frequent T4. Our study reveals importance of the presence of Acanthamoeba in the livestock environment and the need for further studies to associate the presence of these organisms and the role in veterinary pathology. To the best of our knowledge, this is the first report demonstrating the presence Acanthamoeba in La Pampa province and the first study at the genotype level in Argentina. © 2017 Asociación Argentina de Microbiología. INPR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03257541_v_n_p_Rojas
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Acanthamoeba Genotyping La Pampa PCR Watering trough Acanthamoeba Argentina clinical article genotype genus livestock nonhuman polymerase chain reaction veterinary pathology
spellingShingle	Acanthamoeba Genotyping La Pampa PCR Watering trough Acanthamoeba Argentina clinical article genotype genus livestock nonhuman polymerase chain reaction veterinary pathology Rojas, M.D.C. Rodríguez Fermepín, M. Gracia Martínez, F. Costamagna, S.R. Presence of Acanthamoeba spp. in watering trough in La Pampa province, Argentina
topic_facet	Acanthamoeba Genotyping La Pampa PCR Watering trough Acanthamoeba Argentina clinical article genotype genus livestock nonhuman polymerase chain reaction veterinary pathology
description	Free-living Amoebae of Acanthamoeba genus include non-pathogenic and pathogenic strains that are currently classified in 18 different genotypes, T1-T18. In this study, a survey was carried out to evaluate the presence of Acanthamoeba strains inwatering trough sample in La Pampa province, Argentina. Sample were inoculated onto non-nutrient agar plates and were checked for the presence of Acanthamoeba. Polymerase chain reaction was performed with genus-specific primers JDP1/JDP2, followed by direct sequencing of the polymerase chain reaction product for molecular identification. Sequencing results revealed the presence of T4, T5 and T15 genotypes within the studied samples. Sequencing revealed presence of T4, T5 and T15 in the samples studied genotypes, the most frequent T4. Our study reveals importance of the presence of Acanthamoeba in the livestock environment and the need for further studies to associate the presence of these organisms and the role in veterinary pathology. To the best of our knowledge, this is the first report demonstrating the presence Acanthamoeba in La Pampa province and the first study at the genotype level in Argentina. © 2017 Asociación Argentina de Microbiología.
format	INPR
author	Rojas, M.D.C. Rodríguez Fermepín, M. Gracia Martínez, F. Costamagna, S.R.
author_facet	Rojas, M.D.C. Rodríguez Fermepín, M. Gracia Martínez, F. Costamagna, S.R.
author_sort	Rojas, M.D.C.
title	Presence of Acanthamoeba spp. in watering trough in La Pampa province, Argentina
title_short	Presence of Acanthamoeba spp. in watering trough in La Pampa province, Argentina
title_full	Presence of Acanthamoeba spp. in watering trough in La Pampa province, Argentina
title_fullStr	Presence of Acanthamoeba spp. in watering trough in La Pampa province, Argentina
title_full_unstemmed	Presence of Acanthamoeba spp. in watering trough in La Pampa province, Argentina
title_sort	presence of acanthamoeba spp. in watering trough in la pampa province, argentina
url	http://hdl.handle.net/20.500.12110/paper_03257541_v_n_p_Rojas
work_keys_str_mv	AT rojasmdc presenceofacanthamoebasppinwateringtroughinlapampaprovinceargentina AT rodriguezfermepinm presenceofacanthamoebasppinwateringtroughinlapampaprovinceargentina AT graciamartinezf presenceofacanthamoebasppinwateringtroughinlapampaprovinceargentina AT costamagnasr presenceofacanthamoebasppinwateringtroughinlapampaprovinceargentina
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Presence of Acanthamoeba spp. in watering trough in La Pampa province, Argentina

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