Phosphotransbutyrylase expression in Bacillus megaterium
The molecular analysis of a genomic region of B. megaterium revealed the presence of a gene coding for the enzyme phosphotransbutyrylase (Ptb). The enzyme activity was measured throughout the different phases of growth in B. megaterium, and its activity was found to be maximal in the late exponentia...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_03438651_v42_n5_p345_Vazquez |
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todo:paper_03438651_v42_n5_p345_Vazquez2023-10-03T15:26:15Z Phosphotransbutyrylase expression in Bacillus megaterium Vazquez, G.J. Pettinari, M.J. Méndez, B.S. bacterial enzyme isoleucine phosphotransbutyrylase unclassified drug valine article Bacillus megaterium bacterial growth enzyme activity enzyme substrate gene expression regulation nonhuman nucleotide sequence priority journal protein expression regulatory mechanism Amino Acids, Branched-Chain Bacillus megaterium Culture Media DNA-Binding Proteins DNA-Directed RNA Polymerases Gene Expression Regulation, Bacterial Kinetics Phosphate Acetyltransferase RNA Polymerase Sigma 54 Sigma Factor Bacillus megaterium Bacteria (microorganisms) Posibacteria Prokaryota The molecular analysis of a genomic region of B. megaterium revealed the presence of a gene coding for the enzyme phosphotransbutyrylase (Ptb). The enzyme activity was measured throughout the different phases of growth in B. megaterium, and its activity was found to be maximal in the late exponential growth phase. The branched amino acids isoleucine and valine activated Ptb expression. PtbBm was capable of using butyryl-CoA and 2-methyl-propionyl CoA as substrates. ActBm, a σ54 regulator from B. megaterium whose gene is situated upstream from the ptb gene, activated its expression. Fil:Vazquez, G.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pettinari, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Méndez, B.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03438651_v42_n5_p345_Vazquez |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
bacterial enzyme isoleucine phosphotransbutyrylase unclassified drug valine article Bacillus megaterium bacterial growth enzyme activity enzyme substrate gene expression regulation nonhuman nucleotide sequence priority journal protein expression regulatory mechanism Amino Acids, Branched-Chain Bacillus megaterium Culture Media DNA-Binding Proteins DNA-Directed RNA Polymerases Gene Expression Regulation, Bacterial Kinetics Phosphate Acetyltransferase RNA Polymerase Sigma 54 Sigma Factor Bacillus megaterium Bacteria (microorganisms) Posibacteria Prokaryota |
| spellingShingle |
bacterial enzyme isoleucine phosphotransbutyrylase unclassified drug valine article Bacillus megaterium bacterial growth enzyme activity enzyme substrate gene expression regulation nonhuman nucleotide sequence priority journal protein expression regulatory mechanism Amino Acids, Branched-Chain Bacillus megaterium Culture Media DNA-Binding Proteins DNA-Directed RNA Polymerases Gene Expression Regulation, Bacterial Kinetics Phosphate Acetyltransferase RNA Polymerase Sigma 54 Sigma Factor Bacillus megaterium Bacteria (microorganisms) Posibacteria Prokaryota Vazquez, G.J. Pettinari, M.J. Méndez, B.S. Phosphotransbutyrylase expression in Bacillus megaterium |
| topic_facet |
bacterial enzyme isoleucine phosphotransbutyrylase unclassified drug valine article Bacillus megaterium bacterial growth enzyme activity enzyme substrate gene expression regulation nonhuman nucleotide sequence priority journal protein expression regulatory mechanism Amino Acids, Branched-Chain Bacillus megaterium Culture Media DNA-Binding Proteins DNA-Directed RNA Polymerases Gene Expression Regulation, Bacterial Kinetics Phosphate Acetyltransferase RNA Polymerase Sigma 54 Sigma Factor Bacillus megaterium Bacteria (microorganisms) Posibacteria Prokaryota |
| description |
The molecular analysis of a genomic region of B. megaterium revealed the presence of a gene coding for the enzyme phosphotransbutyrylase (Ptb). The enzyme activity was measured throughout the different phases of growth in B. megaterium, and its activity was found to be maximal in the late exponential growth phase. The branched amino acids isoleucine and valine activated Ptb expression. PtbBm was capable of using butyryl-CoA and 2-methyl-propionyl CoA as substrates. ActBm, a σ54 regulator from B. megaterium whose gene is situated upstream from the ptb gene, activated its expression. |
| format |
JOUR |
| author |
Vazquez, G.J. Pettinari, M.J. Méndez, B.S. |
| author_facet |
Vazquez, G.J. Pettinari, M.J. Méndez, B.S. |
| author_sort |
Vazquez, G.J. |
| title |
Phosphotransbutyrylase expression in Bacillus megaterium |
| title_short |
Phosphotransbutyrylase expression in Bacillus megaterium |
| title_full |
Phosphotransbutyrylase expression in Bacillus megaterium |
| title_fullStr |
Phosphotransbutyrylase expression in Bacillus megaterium |
| title_full_unstemmed |
Phosphotransbutyrylase expression in Bacillus megaterium |
| title_sort |
phosphotransbutyrylase expression in bacillus megaterium |
| url |
http://hdl.handle.net/20.500.12110/paper_03438651_v42_n5_p345_Vazquez |
| work_keys_str_mv |
AT vazquezgj phosphotransbutyrylaseexpressioninbacillusmegaterium AT pettinarimj phosphotransbutyrylaseexpressioninbacillusmegaterium AT mendezbs phosphotransbutyrylaseexpressioninbacillusmegaterium |
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1807320193611857920 |